Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites

Stefanache, Camelia Paula; Imseng, Nicole; Meier, Beat; Tanase, Catalin; Eibl-Schindler, Regine; Peter, Samuel; Wolfram, Evelyn

doi:10.1055/s-0033-1352447

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPTLC identification and densitometric assay: HPTLC chromatographic study was done according to literature [8][9][10] using the equipment CAMAG LINOMAT IV, TLC 3 Scanner and as software WINCATS Planar Chromatography Manager.…”

Section: Qualitative Analysismentioning

confidence: 99%

Studies on Polyphenols Isolated from Branches of Prunus spinosa L. Species

Ciupercǎ¹,

Ţebrencu²,

Ionescu³

et al. 2019

Rev. Chim.

View full text Add to dashboard Cite

The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. (Rosaceae), in order to quantify polyphenols and tannins using instrumental methods (UV-Vis spectrometry and HPTLC densitometry). The qualitative analysis on biomass revealed the presence of valuable compounds such as polyphenols, flavonoids and tannins. HPTLC fingerprinting of crude methanol extract showed several peaks, with different Rf values, corresponding to phytocompounds such as chlorogenic acid (Rf 0.52), neochlorogenic acid (Rf 0.58), caffeic acid (Rf 0.95) and protocatechuic acid (Rf 0.96). The quantitative analysis revealed that the branches of P. spinosa contain polyphenols (2.97�0.059%g gallic acid equivalent/g dried vegetal material) and tannins (0.90�0.033%g tannic acid equivalent/g dried vegetal material). The HPTLC densitometry analysis offers information about the amount of neochlorogenic acid in the vegetal material 0.12% g/g. The content in polyphenols and tannins suggests the possibilities to use this species to separate compounds with antioxidant potential. These results open perspectives for complex valorization of Prunus spinosa species, a bioresource known only for phytotherapeutical and nutritional potential of their fruits.

show abstract

Section: Qualitative Analysismentioning

confidence: 99%