Arsenic Contamination in Agricultural Soil Reduces Metabolic Activity of Total and Free-Living Nitrogen-Fixing Bacteria as Revealed by Real-Time qPCR

Chakraborty, Arindam; Bhakat, Kiron; Islam, Ekramul

doi:10.1080/15320383.2017.1413637

Cited by 10 publications

(2 citation statements)

References 42 publications

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“…In this study, particularly, we observed a signi cant increase of arcA, narH, and narJ signal intensity with the silicate fertilization. Arsenic contamination in arable soils commonly reduces microbial metabolic activities for nutrient acquisition [25]. The common nutrients that are often limited are C, N, and P. We observed an increase in gene signal intensities involved in N and P, but not C acquisition with the silicate fertilization.…”

Section: Discussionmentioning

confidence: 60%

Silicate Fertilization Improves Microbial Functional Potential For Stress Tolerance in Arsenic-Enriched Rice Cropping Systems

Das

Kim

Lee

et al. 2021

Preprint

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Background: The host plant and its rhizosphere microbiome are similarly exposed to abiotic stresses under arsenic (As)-enriched cropping systems. Since silicon (Si) fertilization is effective in alleviating As-induced stresses in plants, and plant-microbe interactions are tightly coupled, we hypothesized that Si-fertilization would improve soil microbial functional potential to environmental stress tolerance, which was surprisingly not yet studied. With the help of high throughput metagenome, microarray and analyzing plant impacts on soil microbiome and the environment, we tested the hypothesis in two geographically different rice (i.e., Japonica and Indica) grown on As- enriched soils.Results: Silicate fertilization in rice grown on As-enriched soils altered rhizosphere bacterial communities and increased several commensal microorganisms and their genetic potential to tolerate oxidative stress, osmotic stress, oxygen limitation, nitrogen and phosphate limitation, heat and cold shock, and radiation stress. The stress resistant microbial communities shifted with the changes in rhizosphere nutrient flows and cumulative plant impacts on the soil environment.Conclusions: The study highlights a thus-far unexplored behavior of Si-fertilization to improve microbial stress resilience under As-laden cropping systems and open up promising avenue to further study how commonalities in plant-microbe signaling in response to Si-fertilization alleviates As-induced stresses in agro-systems.

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Section: Discussionmentioning

confidence: 60%

Silicate Fertilization Improves Microbial Functional Potential For Stress Tolerance in Arsenic-Enriched Rice Cropping Systems

Das

Kim

Lee

et al. 2021

Preprint

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“…The quantity and quality of DNA were first detected by agarose gel electrophoresis (1%) and then determined by NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific; Waltham, MA, USA). After DNA extraction, the relative abundance of the 16S rRNA gene was detected by real-time fluorescence quantitative PCR (qPCR) [49]. For each soil sample, the qPCR reaction was repeated four times.…”

Section: Dna Extraction Pcr Amplification and Miseq Sequencingmentioning

confidence: 99%

pH-Related Changes in Soil Bacterial Communities in the Sanjiang Plain, Northeast China

Wang,

Pu,

Wang

et al. 2023

Microorganisms

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Soil bacteria are crucial components of terrestrial ecosystems, playing an important role in soil biogeochemical cycles. Although bacterial community diversity and composition are regulated by many abiotic and biotic factors, how soil physiochemical properties impact the soil bacteria community diversity and composition in wetland ecosystems remains largely unknown. In this study, we used high-throughput sequencing technology to investigate the diversity and composition of a soil bacterial community, as well as used the structural equation modeling (SEM) method to investigate the relationships of the soil’s physicochemical properties (i.e., soil pH, soil organic carbon (SOC), total nitrogen (TN), ammonium nitrogen (NH4+N), electrical conductivity (EC) and nitrate nitrogen (NO3−N)), and soil bacterial community structures in three typical wetland sites in the Sanjiang Plain wetland. Our results showed that the soil physicochemical properties significantly changed the α and β-diversity of the soil bacteria communities, e.g., soil TN, NH4+N, NO3−N, and SOC were the main soil factors affecting the soil bacterial α-diversity. The soil TN and pH were the key soil factors affecting the soil bacterial community. Our results suggest that changes in soil pH indirectly affect soil bacterial communities by altering the soil nitrogenous nutrient content.

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Elevated level of arsenic negatively influences nifH gene expression of isolated soil bacteria in culture condition as well as soil system

Chakraborty

Chowdhury

Bhakat

et al. 2019

Environ Geochem Health

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Arsenic Contamination in Agricultural Soil Reduces Metabolic Activity of Total and Free-Living Nitrogen-Fixing Bacteria as Revealed by Real-Time qPCR

Cited by 10 publications

References 42 publications

Silicate Fertilization Improves Microbial Functional Potential For Stress Tolerance in Arsenic-Enriched Rice Cropping Systems

Silicate Fertilization Improves Microbial Functional Potential For Stress Tolerance in Arsenic-Enriched Rice Cropping Systems

pH-Related Changes in Soil Bacterial Communities in the Sanjiang Plain, Northeast China

Elevated level of arsenic negatively influences nifH gene expression of isolated soil bacteria in culture condition as well as soil system

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