Arsenic exposure during porcine oocyte maturation negatively affects embryonic development by triggering oxidative stress-induced mitochondrial dysfunction and apoptosis

Kang, Ho; Jeong, Pil-Soo; Kim, Min Ju; Joo, Ye Eun; Gwon, Min-Ah; Jeon, Se-Been; Song, Bong-Seok; Kim, Sun-Uk; Lee, Sanghoon; Sim, Bo-Woong

doi:10.1016/j.tox.2022.153314

Cited by 14 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cumulus cells after 44 h of IVM were removed by gentle pipetting in PB1 medium [0.4% bovine serum albumin (BSA) in Dulbecco’s phosphate-buffered saline (DPBS; Gibco, Grand Island, NY, USA)] containing 0.1% hyaluronidase. According to previous studies [ 17 , 18 ], denuded oocytes were considered as metaphase II (MII), immature, or degenerate using a stereomicroscope (Nikon Corp.)…”

Section: Methodsmentioning

confidence: 99%

Restoration of Developmental Competence in Low-Quality Porcine Cumulus–Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation

Jeong

Kang

Song

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

The sonic hedgehog (SHH) pathway is an important signaling pathway for mammalian ovarian folliculogenesis and oocyte maturation. A previous study demonstrated that low-quality porcine cumulus–oocyte complexes (COCs) have low developmental competence, with lower SHH signaling protein expression before and after in vitro maturation (IVM) than high-quality COCs. However, there is no reported evidence on the restorative effects of SHH protein supplementation during the IVM of low-quality porcine COCs. Therefore, this study investigated the effects of SHH protein supplementation on the IVM of low-quality porcine COCs, as assessed by brilliant cresyl blue (BCB) staining. To examine this, we designed four groups: (i) BCB− (low-quality), (ii) BCB− + SHH, (iii) BCB+ (high-quality), and (iv) BCB+ + SHH. While the supplementation of SHH protein with high-quality COCs had no effect, supplementation with low-quality COCs significantly improved cumulus cell expansion, metaphase II rate, and subsequent embryo development following parthenogenetic activation. Our results provide the first evidence that the low developmental competence of low-quality porcine COCs can be improved by supplementation with the SHH protein. These results indicate that an active SHH signaling pathway is required for the acquisition of developmental competence in porcine COCs.

show abstract

Section: Methodsmentioning

confidence: 99%

Restoration of Developmental Competence in Low-Quality Porcine Cumulus–Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation

Jeong

Kang

Song

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Arsenic is also be identified as a neurotoxin, which affects children’s IQ and invokes many adverse health consequences (Figure B). Additionally, many factors are involved in the toxic mechanisms of arsenic, including inflammation and oxidative stress, , protein degradation, autophagy, ,− mitochondrial dysfunction, endoplasmic reticulum (ER) stress, and apoptosis. , Recently, miRNAs dysregulation was considered to be one of the important mechanisms by which arsenic leads to toxicity and human diseases, although the exact mechanism remains unclear. ,,,, The following subsections highlight the role of miRNAs and regulatory mechanisms of miRNAs in arsenic-induced human diseases, including cancers (skin, lung, liver, kidney, bladder, and prostatic cancer), CVD, and other human diseases (DM, skin lesion, liver, lung, neurodegenerative, and kidney diseases).…”

Section: Arsenicmentioning

confidence: 99%

“…Arsenic is also be identified as a neurotoxin, which affects children's IQ and invokes many adverse health consequences 96 (Figure 2B). Additionally, many factors are involved in the toxic mechanisms of arsenic, including inflammation and oxidative stress, 25,27 protein degradation, 97 autophagy, 25,28−30 mitochondrial dysfunction, 98 endoplasmic reticulum (ER) stress, 99 and apoptosis. 95,100 Recently, miRNAs dysregulation was considered to be one of the important mechanisms by which arsenic leads to toxicity and human diseases, although the exact mechanism remains unclear.…”

Section: Arsenicmentioning

confidence: 99%

“…TLR3 mediated TRIF signaling was involved in the immunomodulatory effect of plant miR-168. Inflammation is a key mechanism by which arsenic leads to toxicity and human diseases. , Therefore, these plant miRNAs can be used to alleviate inflammation and inhibit the release of inflammatory factors invoked by arsenic exposure.…”

Section: Exogenous Mirnas and Human Diseasesmentioning

confidence: 99%

“…More than 50 million people from West Bengal and Bangladesh were exposed to arsenic levels above 50 μg/L, which caused frequent arsenic poisoning and adverse effects on human health . A growing body of literature suggests that arsenic disrupts a series of cellular processes, including oxidative stress, cell proliferation, inflammation, and autophagy and apoptosis. ,− Arsenic exposure can lead to many adverse health effects of diabetes mellitus (DM), − skin lesions, , kidney disease, , neurological impairment, , cancers, , male reproductive injury, and cardiovascular disease (CVD) as cardiac arrhythmias, endothelial dysfunction, and ischemic heart failure. , …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Role of microRNAs in Arsenic-Induced Human Diseases: A Review

Liu,

Lei

2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are noncoding RNAs with 20–22 nucleotides, which are encoded by endogenous genes and are capable of targeting the majority of human mRNAs. Arsenic is regarded as a human carcinogen, which can lead to many adverse health effects including diabetes, skin lesions, kidney disease, neurological impairment, male reproductive injury, and cardiovascular disease (CVD) such as cardiac arrhythmias, ischemic heart failure, and endothelial dysfunction. miRNAs can act as tumor suppressors and oncogenes via directly targeting oncogenes or tumor suppressors. Recently, miRNA dysregulation was considered to be an important mechanism of arsenic-induced human diseases and a potential biomarker to predict the diseases caused by arsenic exposure. Endogenic miRNAs such as miR-21, the miR-200 family, miR-155, and the let-7 family are involved in arsenic-induced human disease by inducing translational repression or RNA degradation and influencing multiple pathways, including mTOR/Arg 1, HIF-1α/VEGF, AKT, c-Myc, MAPK, Wnt, and PI3K pathways. Additionally, exogenous miRNAs derived from plants, such as miR-34a, miR-159, miR-2911, miR-159a, miR-156c, miR-168, etc., among others, can be transported from blood to specific tissue/organ systems in vivo. These exogenous miRNAs might be critical players in the treatment of human diseases by regulating host gene expression. This review summarizes the regulatory mechanisms of miRNAs in arsenic-induced human diseases, including cancers, CVD, and other human diseases. These special miRNAs could serve as potential biomarkers in the management and treatment of human diseases linked to arsenic exposure. Finally, the protective action of exogenous miRNAs, including antitumor, anti-inflammatory, anti-CVD, antioxidant stress, and antivirus are described.

show abstract

Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats

Mondal

Pal

Biswas

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Arsenic exposure during porcine oocyte maturation negatively affects embryonic development by triggering oxidative stress-induced mitochondrial dysfunction and apoptosis

Cited by 14 publications

References 42 publications

Restoration of Developmental Competence in Low-Quality Porcine Cumulus–Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation

Restoration of Developmental Competence in Low-Quality Porcine Cumulus–Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation

The Role of microRNAs in Arsenic-Induced Human Diseases: A Review

Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats

Contact Info

Product

Resources

About