Arsenicitalea aurantiaca gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae, isolated from high-arsenic sediment

Mu, Yao; Zhou, Lingli; Zeng, Xian‐Chun; Liu, Lan; Pan, Yunfan; Chen, Xiaoming; Wang, Jianbo; Li, Shan-Hui; Li, Wenjun; Wang, Yanxin

doi:10.1099/ijsem.0.001543

Cited by 21 publications

(15 citation statements)

References 21 publications

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“…The polar lipid profile of strain LMO-2 T comprised diphosphatidylglycerol, phosphatidylglycerol, phospholipid and five unknown polar lipids (Fig. S3), which is consistent with the type species of the genera Devosia, Youhaiella, Pelagibacterium and Paradevosia, while the genus Arsenicitalea does not contain any unknown phospholipids [2]. As mentioned above, phylogenetic and genomic analysis suggested that strain LMO-2 T represents a novel genus that is closely to several genera of the family Devosiaceae: including Arsenicitalea [2], Pelagibacterium [4], Devosia [26], Paradevosia [27] and Youhaiella [54].…”

Section: Physiology and Chemotaxonomysupporting

confidence: 69%

“…Growth at various temperatures (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 °C) and different NaCl concentrations (0, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15 %, w/v) were examined following growth on R2A agar medium. The pH range for growth was measured in R2A broth at various pH values (pH 4.0–12.0 at intervals of 0.5 pH unit) for 7 days by using the buffer systems Na 2 HPO 4 /citric acid (for pH 5.0–7.0), Tris/HCl (pH 8.0–9.0) and Na 2 CO 3 /NaHCO 3 (pH 9.0–11.0), respectively, in R2A broth medium at the concentration of 40 mM [2, 4]. Oxidase activity was determined by using an oxidase reagent kit (bioMérieux).…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…The family Devosiaceae currently contains over 35 validly published species and are mostly Gram-negative and motile. The most distinctive features reported for the members of the family Devosiaceae are utilization or degradation of persistent hydrocarbons and organic pollutants such as hexachlorocyclohexane [5–7], naphthalene [8] and arsenic [2]. In this study, we established the taxonomic position of a new genus and species within the family Devosiaceae , with the proposed name Mariluticola halotolerans gen. nov., sp.…”

Section: Introductionmentioning

confidence: 99%

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Mariluticola halotolerans gen. nov., sp. nov., a novel member of the family Devosiaceae isolated from South China Sea sediment

Ishaq

Ahmad

Liang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel member of class Alphaproteobacteria was isolated from marine sediment of the South China Sea. Cells of strain LMO-2T were Gram-stain negative, greyish in colour, motile, with a single lateral flagellum and short rod in shape with a slight curve. Strain LMO-2T was positive for oxidase and negative for catalase. The bacterium grew aerobically at 10–40 °C (optimum, 25–30 °C), pH 5.5–10.0 (optimum, pH 7.0) and 0–9 % NaCl (w/v; optimum, 2–3 %). Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of the whole genome sequence indicated that strain LMO-2T represents a new genus and a new species within the family Devosiaceae , class Alphaproteobacteria , phylum Pseudomonadota . Comparisons of the 16S rRNA gene sequences of strain LMO-2T showed 94.8 % similarity to its closest relative. The genome size is ~3.45 Mbp with a DNA G+C content of 58.17 mol%. The strain possesses potential capability for the degradation of complex organic matter, i.e. fatty acid and benzoate. The predominant cellular fatty acids (>10 %) were C16 : 0 and C18 : 1 ω7c 11-methyl. The sole respiratory quinone was ubiquinone-10. The major identified polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phospholipid. Based on the polyphasic taxonomic data, strain LMO-2T represents a novel genus and a novel species for which the name Mariluticola halotolerans gen. nov., sp. nov., was proposed in the family Devosiaceae . The type strain is LMO-2T (=CGMCC 1.19273T=JCM 34934T).

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Section: Physiology and Chemotaxonomysupporting

confidence: 69%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mariluticola halotolerans gen. nov., sp. nov., a novel member of the family Devosiaceae isolated from South China Sea sediment

Ishaq

Ahmad

Liang

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…Multiple sequence alignments were conducted using the ClustalW2 software as described elsewhere ( Zhang et al, 2015 ; Mu et al, 2016a ). A phylogenetic tree of the obtained ArsMs and their closely related homologues was constructed using the maximum likelihood method implemented in MEGA 6.0 as described previously ( Xu et al, 2014 ; Mu et al, 2016b ).…”

Section: Methodsmentioning

confidence: 99%

Microbially Mediated Methylation of Arsenic in the Arsenic-Rich Soils and Sediments of Jianghan Plain

et al. 2018

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Almost nothing is known about the activities and diversities of microbial communities involved in As methylation in arsenic-rich shallow and deep sediments; the correlations between As biomethylation and environmental parameters also remain to be elucidated. To address these issues, we collected 9 arsenic-rich soil/sediment samples from the depths of 1, 30, 65, 95, 114, 135, 175, 200, and 223 m in Jianghan Plain, China. We used microcosm assays to determine the As-methylating activities of the microbial communities in the samples. To exclude false negative results, we amended the microcosms with 0.2 mM As(III) and 20.0 mM lactate. The results indicated that the microbial communities in all of the samples significantly catalyzed arsenic methylation. The arsM genes were detectable from all the samples with the exception of 175 m, and 90 different arsM genes were identified. All of these genes code for new or new-type ArsM proteins, suggesting that new As-methylating microorganisms are widely distributed in the samples from shallow to deep sediments. To determine whether microbial biomethylation of As occurs in the sediments under natural geochemical conditions, we conducted microcosm assays without exogenous As and carbons. After 80.0 days of incubation, approximately 4.5–15.5 μg/L DMAsV were detected in all of the microcosms with the exception of that from 30 m, and 2.0–9.0 μg/L MMAsV were detected in the microcosms of 65, 114, 135, 175, 200, and 223 m; moreover, approximately 18.7–151.5 μg/L soluble As(V) were detected from the nine sediment samples. This suggests that approximately 5.3, 0, 8.1, 28.9, 18.0, 8.7, 13.8, 10.2, and 14.9% of total dissolved As were methylated by the microbial communities in the sediment samples from 1, 30, 65, 95, 114, 135, 175, 200, and 223 m, respectively. The concentrations of biogenic DMAsV show significant positive correlations with the depths of sediments, and negative correlations with the environmental NH4+ and NaCl concentrations, but show no significant correlations with other environmental parameters, such as NO3-, SO42+, TOC, TON, Fe, Sb, Cu, K, Ca, Mg, Mn, and Al. This work helps to better understand the biogeochemical cycles of arsenic in arsenic-rich shallow and deep sediments.

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“…Luteolibacter have previously reported as one of the 5 most abundant genera in arsenic contaminated soil 63 and Devosia were shown to exhibit high sequence similarity to arsenic tolerant organisms isolated from arsenic rich environments. 64 Therefore, both the genomic matches of arsM OTUs and the correlated copy number patterns in Group I, II, III, and IV arsM and genera level 16S rRNA OTUs present potential targets for identification of arsM organisms and the study of arsenic transformation in paddy soil.…”

Section: Environmental Science and Technologymentioning

confidence: 99%

Inhibition of Microbial Methylation via arsM in the Rhizosphere: Arsenic Speciation in the Soil to Plant Continuum

Afroz

Carey

et al. 2019

Environ. Sci. Technol.

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The interplay between rice roots and manuring with respect to arsenic speciation, subsequent assimilation into roots, and translocation to shoots in paddy soil was investigated, alongside bacterial diversity characterization. Planting increased soil Eh and decreased soil solution arsenic species: inorganic arsenic (iAs), monomethylarsonic acid (MMA), trimethylarsenic oxide (TMAO) and dimethylarsinic acid (DMA). Presence of plant roots increased copy number of Clostridium and Tumebacillus 16S rRNA as well as Streptomyces arsenic methylating gene (arsM), but decreased Acidobacteria_GP1 16S rRNA and R. palustris BisB5 arsM. Sum of arsenic species decreased under root influence due to the interplay of inorganic arsenic mobilization in bulk soil under anaerobic and immobilization under oxygenated rhizospheric conditions. Manuring increased all soil solution arsenic species (>90%), shoot total arsenic (60%), copy number of Geobacter 16S rRNA and R. palustris TIE-1 arsM, indicative of a shift towards microbes with iron reduction and oxidation as well as arsenic methylation capabilities.

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Arsenicitalea aurantiaca gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae, isolated from high-arsenic sediment

Cited by 21 publications

References 21 publications

Mariluticola halotolerans gen. nov., sp. nov., a novel member of the family Devosiaceae isolated from South China Sea sediment

Mariluticola halotolerans gen. nov., sp. nov., a novel member of the family Devosiaceae isolated from South China Sea sediment

Microbially Mediated Methylation of Arsenic in the Arsenic-Rich Soils and Sediments of Jianghan Plain

Inhibition of Microbial Methylation via arsM in the Rhizosphere: Arsenic Speciation in the Soil to Plant Continuum

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