Artefact formation of formaldehyde in milk powders: Impact of analytical conditions

Bessaire, Thomas; Savoy, Marie-Claude; Tarres, Adrienne; Mujahid, Claudia; Goldmann, Till; Perrin, Irène; Mottier, Pascal

doi:10.1016/j.foodcont.2018.05.029

Cited by 4 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method showed at least four-fold higher sensitivity for FA in milk than the current DNPH derivatization method ( Figure 6). As it has been shown that an artefact of FA can be formed in milk powders at the higher temperature (60 C) used in the DNPH derivatization method in the presence of iron and ascorbic acid, 22 the GP derivatization of FA takes place at a lower temperature (37 C).…”

Section: Discussionmentioning

confidence: 99%

“…This method showed at least four‐fold higher sensitivity for FA in milk than the current DNPH derivatization method (Figure 6). As it has been shown that an artefact of FA can be formed in milk powders at the higher temperature (60°C) used in the DNPH derivatization method in the presence of iron and ascorbic acid, 22 the GP derivatization of FA takes place at a lower temperature (37°C). Although the sensitivity is similar in the two methods, GP derivatization is easier to use than DNPH derivatization, and the reaction is rapid, requires no purification procedures and allows for increased sample throughput.…”

Section: Discussionmentioning

confidence: 99%

“…However, these methods lack the required sensitivity and specificity and often a high sample volume is needed. Although the best way to analyze compounds at ng to pg levels in foods is mass spectrometry (MS)‐based quantification using stable isotope dilution, a few methods have been developed that use labelled FA as an internal standard for quantification of FA 17,22 . Since, however, a trace amount of FA is known to be present in food samples like milk, the published methods fail to determine the endogenous amount accurately.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simple click chemistry‐based derivatization to quantify endogenous formaldehyde in milk using ultra‐high‐performance liquid chromatography/tandem mass spectrometry in selected reaction monitoring mode

Shetty

Rangiah

2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Rationale Formaldehyde (FA) exposure via environmental pollution or through the food chain poses a serious threat to human health, especially in developing countries like India. Although the addition of FA to food is proscribed, it is often illegally added to foods such as milk to increase the shelf‐life. There are challenges in differentiating the endogenous FA content in milk from externally added FA. Method We have developed a simple method using ultra‐high‐performance liquid chromatography/tandem mass spectrometry in selected reaction monitoring mode (UHPLC/MS/SRM) for the absolute quantification of endogenous FA in milk. The steps include fat removal, protein precipitation using acid, and spiking with labelled FA (FA*), followed by simple click chemistry‐based derivatization using Girard P reagent (GP) and final analysis. Results A standard curve with FA* was constructed and used for the calculation of endogenous FA in milk. The optimal conditions for the derivatization reaction using 500 μL of milk were: GP, 50 μg; temperature, 37°C; time, 60 min; and 0.1% HCl. The validation parameters such as accuracy (95.84 to 99.73%), precision (2.84 to 8.02%) and spiked recovery (>95%) are within the FDA guidelines. This method is highly sensitive [limit of detection (LOD) of 1 ng/mL] with a dynamic range of 3.12 to 200 ng/mL. The endogenous FA level in pasteurized cow milk is 70 ng/mL (n = 60). The FA content in raw milk samples from cow, goat and buffalo (each n = 10) varied from 134 to 255 ng/mL. Conclusions This method is precise and sufficiently sensitive to quantify endogenous FA in milk samples using a minimal sample volume. As it involves simple sample preparation steps, it can be used routinely to quantify endogenous FA.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple click chemistry‐based derivatization to quantify endogenous formaldehyde in milk using ultra‐high‐performance liquid chromatography/tandem mass spectrometry in selected reaction monitoring mode

Shetty

Rangiah

2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

Isopropyl-naphthylamide-hydrazine as a novel fluorescent reagent for ultrasensitive determination of carbonyl species on UPLC

Shi

Yun-yu

Zhou

et al. 2022

Microchemical Journal

View full text Add to dashboard Cite

Assessment of Lipid Peroxidation Products in Adult Formulas: GC-MS Determination of Carbonyl and Volatile Compounds Under Different Storage Conditions

Custodio-Mendoza,

Rangel Silva,

Kurek

et al. 2024

Foods

View full text Add to dashboard Cite

The occurrence of carbonyl compounds and volatile organic compounds (VOCs) in adult formulas is a critical issue in product safety and quality. This research manuscript reports the determination of targeted and untargeted carbonyl compounds and VOCs in adult formulas stored at different temperatures (room temperature, 4 °C, and 60 °C) over one month. Gas chromatography-mass spectrometry was utilized for the sample analysis. Ultrasound-assisted dispersive liquid–liquid microextraction at 60 °C for 20 min facilitated the extraction of six carbonyl compounds, while headspace solid-phase microextraction (HS-SPME) was employed for the determination of untargeted VOCs using a DVB/CAR/PDMS fiber, involving 15 min of equilibration and 45 min of extraction at 40 °C with magnetic stirring. Analytical features of the methods were assessed according to Food and Drug Administration guidelines, and good limits of detection and quantitation, linearity, accuracy, and precision were achieved. Notably, the highest levels of carbonyl compounds were found in high-protein formulas, with quantifiable levels of malondialdehyde, acrolein, and formaldehyde detected and quantified in 80% of samples. Additionally, significant levels of VOCs such as hexanal and 2-heptanone were found in samples stored at elevated temperatures. These findings suggest the importance of protein content and storage conditions in the levels of carbonyl compounds and VOCs found in adult formulas, with implications for consumer safety and quality control.

show abstract

Artefact formation of formaldehyde in milk powders: Impact of analytical conditions

Cited by 4 publications

References 27 publications

Simple click chemistry‐based derivatization to quantify endogenous formaldehyde in milk using ultra‐high‐performance liquid chromatography/tandem mass spectrometry in selected reaction monitoring mode

Simple click chemistry‐based derivatization to quantify endogenous formaldehyde in milk using ultra‐high‐performance liquid chromatography/tandem mass spectrometry in selected reaction monitoring mode

Isopropyl-naphthylamide-hydrazine as a novel fluorescent reagent for ultrasensitive determination of carbonyl species on UPLC

Assessment of Lipid Peroxidation Products in Adult Formulas: GC-MS Determination of Carbonyl and Volatile Compounds Under Different Storage Conditions

Contact Info

Product

Resources

About