Artichoke yellow ringspot nepovirus naturally infecting cucumber in Crete

Abstract

An isometric virus was isolated from cucumber plants growing in a plastic house in Crete and showing stunting and bright yellow mosaic of the leaves. Based on host range, properties in crude sap, behaviour during purification, electron microscopy and serology, the virus was identified as an isolate of artichoke yellow ringspot nepovirus. Ecological data corroborate transmission of the virus via the soil.

Broad bean wrinkly seed caused by artichoke yellow ringspot nepovirus

“…The inability of broad bean seedlings to acquire AYRV-F from sterilised soil and the absence of infectivity in soil leachates, are consistent with previous results obtained with the cucumber isolate of AYRV (Avgelis & Vovlas, 1989). On the other hand, the failure of bait trials may also exclude the involvement in spread of a nematode vector in Cretan broad bean fields.…”

“…The host range and symptomatology of AYRV-F were similar to those reported for other isolates of AYRV (i.e. production of enations and severe stunting in cucumber, mottling and top necrosis of C. quinoa and systemic leaf ringspots and line patterns in Nicotiana species (Rana et al, 1980;Avgelis & Vovlas, 1989). AYRV-F, however, induced quite different symptoms in some host species.…”

“…During an extensive survey in North-Eastern Peloponnesus, AYRV was found to occur in a wide range of cultivated plants (including broad bean) and weeds (Kyriakopoulou, Rana & Roca, 1985). Recently AYRV was isolated from cucumbers grown in plastic houses in Crete (Avgelis & Vovlas, 1989).…”

“…Purification and serology AYRVbroad bean virus isolate (AYRV-F) was purified according to Rana et al (1980) using C. quinoa infected plants collected before the appearance of top necrosis (Avgelis & Vovlas, 1989). Partially purified virus preparations were further purified using linear 10-40% sucrose gradients; the virus bands were collected and concentrated by centrifugation at 100 000 g for 4 h. The resulting pellet was resuspended in 0.02 M phosphate buffer pH 7.2.…”

“…Aquadulce, grown in 100 ml plastic pots. Six weeks later filtrates were obtained, centrifuged at 105 000 g for 70 min and the resulting pellets, after resuspension, were tested for infectivity on C. quinoa (Avgelis & Vovlas, 1989). Ten healthy broad bean plants were used as controls.…”