Articular Cartilage Transplantation

Chu, Constance R.; Fr, Convery; Akeson, Wayne H.; Meyers, Marvin H.; Amiel, David

doi:10.1097/00003086-199903000-00019

Cited by 278 publications

(169 citation statements)

References 24 publications

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“…Several different approaches are being investigated as an attempt to repair or regenerate the damaged cartilage. Some of the approaches being used clinically to regenerate cartilage include drilling of articular defects to recruit stem cells from the subchondral bone to participate in repair, mosaic-plasty, and autologous chondrocyte transplantation [1][2][3]. All these approaches, however, have inherent problems in that they cannot repair cartilage full-thickness defects and also there may be morbidity-associated problems and possible disease transmission [2,4,5].…”

Section: Introductionmentioning

confidence: 99%

Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures

Kawamura¹,

Chu²,

Sobajima³

et al. 2005

Experimental Hematology

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102

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Objective. The objective of the present study was to investigate the potential of application of growth factor genes to induce chondrogenic differentiation of human-derived mesenchymal stem cells (MSCs). The growth factor genes evaluated in the present study were transforming growth factor 1 (TGF-β1) and insulin-like growth factor 1 (IGF-1).Methods. Human MSCs were transduced with the adenoviral vectors carrying either TGF-β1 or IGF-1 (AdTGF-β1 and AdIGF-1 respectively) or a combination of both growth factor genes at different multiplicities of infection (MOI) and were then made into pellets. Pellets were also made from nontransduced cells and maintained in culture medium supplemented with 10 ng/mL of TGF-β1. At specified time points, histological analysis, cartilage matrix gene expression, and immunofluorescence were performed to determine the extent of chondrogenic differentiation.Results. MSCs transduced with the AdTGF-β1 demonstrated robust chondrogenic differentiation, while those made from AdIGF-1 did not. AdTGF-β1 pellets demonstrated aggrecan gene expression as early as day 3 of pellet culture, while type II collagen gene expression was detected by day 10 of culture. The AdIGF-1, alone or in combination with TGF-β1 pellets, did not show any type II collagen gene expression at any time point. By immunofluoresecence, type X collagen was distributed throughout the matrix in TGF-β1 protein pellets while the growth factor gene pellets displayed scant staining. Conclusion.The results suggest that sustained administration of TGF-β1 may be more effective in suppressing terminal differentiation than intermittent dosing and thus effective for cartilage repair.

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Section: Introductionmentioning

confidence: 99%

Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures

Kawamura¹,

Chu²,

Sobajima³

et al. 2005

Experimental Hematology

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102

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“…Freezing has the advantage of extending graft availability post-harvest, but it is thought that the clinical performance these grafts are diminished compared to autografts or fresh allografts. Currently, fresh osteochondral allografts are implanted following brief storage at 4°C [6,11,30]. While fresh grafts offer the distinct advantage of maintaining chondrocyte viability, there are significant logistic limitations because to maximize this viability, surgical implantation must be performed within seven days of graft harvest.…”

Section: Introductionmentioning

confidence: 99%

“…First attempts included storage in lactated Ringer's solution, however this storage media lacked essential nutrients (e.g. fetal calf serum) for sustained metabolic function of the chondrocytes [3,6,11,12]. Subsequently, development of storage media containing appropriate antibiotics and nutrients were developed with storage conditions utilizing temperatures at 4 and 37 "C. Although early attempts at storage at 4 "C were unsuccessful [5], later studies utilizing articular cartilage attached to the underlying bone demonstrated the usefulness of cold preservation of cartilage allograft materials stored in media at 4°C [ 1, 15,22,29].…”

Section: Introductionmentioning

confidence: 99%

Prolonged‐fresh preservation of intact whole canine femoral condyles for the potential use as osteochondral allografts

Williams

Virdi

Pylawka

et al. 2005

Journal Orthopaedic Research

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Defects in articular cartilage are often repaired with fresh osteochondral grafts. While fresh allografts provide viable chondrocytes, logistic limitations require surgical implantation within seven days of graft harvest. Here, we provide information on cold preservation of whole intact osteochondral materials that retains cartilage cell viability and function, and histologic and biochemical integrity for 28days. Canine femoral condyles were obtained and stored at 4°C for 14,21 or 28days. At the end of the storage period, cartilage was assessed for cell viability, 35S uptake, proteoglycan content and histologic parameters. The most noticeable histologic change was reduced Safranin-0 near the cartilage surface with 14days of cold preservation, but had recovered with 21 and 28days. Cartilage thicknesses did not vary significantly. Cell viability was >%YO at 14days, 75-98% at 21 days and reduced to 65-90% at 28days. Cell function measures showed that the level of 35S04 incorporation was suppressed in samples stored at 4°C. However, no significant differences were seen among groups at 14,21 or 28days of cold preservation. This data has implications for tissue banking protocols for osteochondral allograft material obtained for transplantation suggesting that cold preserved allograft material be implanted within 28days.

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“…Unlike other tissues, traumatic or degenerative cartilages do not heal well. Cartilage is described as an immunologically privileged tissue, and fresh osteochondral allografts have been proved to be effective and functional for transplantation (Chu et al, 1999;Aubin et al, 2001;Williams et al, 2007;Raikin, 2009). Furthermore, a recent study showed that fresh osteochondral autograft and fresh allograft tissues are not statistically different with respect to bony incorporation, AC composition, and biomechanical properties up to six months after implantation (Glenn et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Zhang

Chen

2012

J. Zhejiang Univ. Sci. B

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Abstract:Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. Vitrification has been thought to be a promising method for successful preservation of articular cartilage (AC), but high concentration cryoprotectants (CPAs) are used which may cause high cellular toxicity. An effective way to reduce CPA toxicity is to increase CPA concentration gradually while the temperature is lowered. Understanding the mechanism of CPA permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide (Me 2 SO) in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were exposed in Me 2 SO solutions for different time (0, 5, 15, 30, 50, 80, and 120 min) at three temperature levels (−10, −20, and −30 °C). The Me 2 SO concentration within the tissue was determined by ultraviolet (UV) spectrophotometry. The diffusion coefficients were estimated to be 0.85×10 −6 , 0.48×10 −6 , and 0.27×10 −6 cm 2 /s at −10, −20, and −30 °C, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to compare two Me 2 SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.

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Articular Cartilage Transplantation

Cited by 278 publications

References 24 publications

Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures

Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures

Prolonged‐fresh preservation of intact whole canine femoral condyles for the potential use as osteochondral allografts

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

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