Artificial Dental Pulp Exposure Injury Up-regulates Antigen-Presenting Cell–related Molecules in Rat Central Nervous System

Kaneko, Tomoyuki; Kaneko, Mitsunori; Chokechanachaisakul, Uraiwan; Kawamura, Jun; Kaneko, Ryuji; Sunakawa, Mitsuhiro; Okiji, Takashi; Suda, Hiroshi

doi:10.1016/j.joen.2009.12.011

Cited by 7 publications

(9 citation statements)

References 32 publications

(41 reference statements)

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“…We have reported that the thalamic glial cells up-regulate antigen-presenting cell-related molecule and N -methyl- D -aspartate receptor mRNAs following artificial exposure of rat pulp, suggesting that central sensi-tization caused via intra-pulpal nociceptive neurons stimulated by inflammation (Kaneko et al ., 2010). To confirm whether central sensitization occurred in the thalamic mediodorsal nucleus, we performed neuronal responsiveness recording of the rat mediodorsal nucleus following mustard oil application to the pulp.…”

Section: Discussionmentioning

confidence: 99%

“…After inferior alveolar nerve-transection of rats, glial fibrillary acidic protein-expressing cells show an increase in the ipsilateral Vc at 7 days, but not 1 day, post-operatively (Okada-Ogawa et al ., 2009). In our recent analysis, where unsealed pulp exposure was carried out for 1 day (Kaneko et al ., 2010), thalamic glial cells, including microglia and astrocytes, increased in number with an up-regulation of antigen-presenting cell-related molecule mRNAs. In the present analysis, we further found that gene expression levels of antigen-presenting cell-related molecules were increased in the contralateral thalamus shortly after the mustard oil application.…”

Section: Discussionmentioning

confidence: 99%

“…At 60 min after the mustard oil application, the thalamus was retrieved from both contralateral and ipsilateral sides. Immunoperoxidase staining with the avidin–biotin–peroxidase complex method was carried out as previously described (Kaneko et al ., 2010). Monoclonal antibody OX6 (anti-rat class II MHC molecules; Serotec, Oxford, UK), N -methyl- D -aspartate receptor (Synaptic Systems, Göttingen, Germany), or anti-glial fibrillary acidic protein (a marker for astrocytes; NeoMarkers, Fremont, CA, USA) was used.…”

Section: Methodsmentioning

confidence: 99%

“…Negative control staining was always carried out in parallel by the incubation of sections with PBS instead of the primary antibodies. The number of OX6- or glial fibrillary acidic protein-expressing cells was counted, as previously described (Kaneko et al ., 2010).…”

Section: Methodsmentioning

confidence: 99%

“…Astrocytes also play an important role in infectious and/or autoimmune diseases of the central nervous system, and can express B7 co-stimulatory molecules in certain circumstances (Zeinstra et al ., 2003). We have recently reported that thalamic glial cells up-regulate antigen-presenting cell-related gene and N -methyl- D -aspartate receptor mRNAs following artificial pulp exposure (Kaneko et al ., 2010). This suggests that central sensitization and subsequent neuron-immune interaction are induced via intra-pulpal nociceptive neurons stimulated by inflammation, and that N -methyl- D -aspartate receptor plays some role in such a neuron-immune interaction.…”

Section: Introductionmentioning

confidence: 99%

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Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp

Kawamura

Kaneko

et al. 2010

J Dent Res

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We have reported that mustard oil application to the rat dental pulp induces neuronal activation in the thalamus. To address the mechanisms involved in the thalamic changes, we performed neuronal responsiveness recording, immunohistochemistry, and molecular biological analysis. After mustard oil application, neuronal responsiveness was increased in the mediodorsal nucleus. When MK801 (an N-methyl-D-aspartate receptor antagonist) was applied to the mediodorsal nucleus, the enhanced responsiveness was decreased. N-methyl-D-aspartate receptor 2D, glial fibrillary acidic protein, and antigen-presenting cell-related gene mRNAs in the contralateral thalamus were up-regulated at 10 minutes after mustard oil application, but were down-regulated within 10 minutes after the antagonist application. OX6-expressing microglia and glial fibrillary acidic protein-expressing astrocytes did not increase until 60 minutes after mustard oil application. These results suggested that the thalamic neurons play some roles in regulating the glial cell activation in the mediodorsal nucleus via N-methyl-D-aspartate receptor 2D during pulp inflammation-induced central sensitization.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp

Kawamura

Kaneko

et al. 2010

J Dent Res

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The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

2018

Journal Cellular Physiology

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Calcium ions (Ca ) is the main element of dental pulp capping materials. Ca signaling plays a crucial role in a myriad of cell activities. An overwhelming array of studies have already reported the experimental and clinical benefits of Ca -enriched materials in the treatment of teeth with accidental vital pulp exposure and incomplete root formation. Thus, Ca signaling has always been an excellent target for the design of various novel biomaterials for use in revitalizing or regenerative endodontic procedures. However, the molecular mechanisms that enable dental pulp cells (DPCs) to detect and respond to extracellular Ca have not been characterized in detail before. In this review, we mainly outline the pathways by which the cell detects and responds to extracellular Ca , as well as the relevant regulatory paths in DPCs and odontoblasts, and discuss the potential role of Ca as a therapeutic tool. Moreover, because DPCs share many of the same functional properties that are found in osteoblasts, some comparisons with bone cells were additionally incorporated into this text.

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Neuron‐glial cell communication in the traumatic stress‐induced immunomodulation

Zhao

Xiao

Kong

et al. 2010

Synapse

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We have previously reported that neuron and glia could collaboratively govern the immunomodulation in traumatic rats. Herein, we characterized the sequential involvement of cortical neuron, microglia, and astrocytes in the traumatic stress-mediated neuroimmune modulation. At day 1 of trauma, transient extracellular signal related kinase 1/2 (ERK1/2) activation was initiated in neuron and microglia, which was accompanied by RSK-1 expression in the cytosol. At day 3 of trauma, persistent ERK1/2 activation occurred in astrocytes, which were destined for the nucleus leading to Elk-1 expression. Furthermore, the functional overlap of ERK1/2 and neuroligin 1 in astrocytes was strengthened at day 3 of trauma and responsible for the recovery from the immnosuppression. These effects could be disrupted by β-neurexin blockade. Altogether, we proposed the mechanism underlying the traumatic stress-induced immunosuppression, in which local activity ensured the initial establishment of neural circuitry in the frontal cortex. ERK1/2-signaling events are required for the temporal and spatial coordination between neuron and glial cells.

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Artificial Dental Pulp Exposure Injury Up-regulates Antigen-Presenting Cell–related Molecules in Rat Central Nervous System

Cited by 7 publications

References 32 publications

Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp

Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

Neuron‐glial cell communication in the traumatic stress‐induced immunomodulation

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Artificial Dental Pulp Exposure Injury Up-regulates Antigen-Presenting Cell–related Molecules in Rat Central Nervous System

Cited by 7 publications

References 32 publications

Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp

Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp

The emerging role of extracellular Ca2+ in osteo/odontogenic differentiation and the involvement of intracellular Ca2+ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

Neuron‐glial cell communication in the traumatic stress‐induced immunomodulation

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The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts