Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass, Dicentrarchus labrax L.

Colombo, Lorenzo; Barbaro, A. D'agostino; Libertini, Angelo; Benedetti, Piero; Francescon, A.; Lombardo, I.

doi:10.1111/j.1439-0426.1995.tb00012.x

Cited by 32 publications

(17 citation statements)

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“…Arti¢cial fertilization was carried out within 10 min from gamete collection, mixing 1 part of the eggs, 4 parts of seawater and 1 part of sperm diluted 1:10 with an extender solution (375 mosmoles kg À1 KCl, pH 7.35) as reported by Colombo, Barbaro, Libertini, Benedetti, Francescon and Lombardo (1995). A full stripping of eggs was performed in all the females and, in case of good egg quality, sperm was immediately collected from the males.…”

Section: Triploidy Induction and Ploidy Determinationmentioning

confidence: 99%

Effects of triploidy on feed efficiency, morphometric indexes and chemical composition of shi drum, Umbrina cirrosa L.

et al. 2006

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Productive performances of triploid (3n) and diploid (2n) shi drum (Umbrina cirrosa L.) were evaluated over an experimental period of 76 days. Fish, selected according to body weight (196 specimens from each ploidy), were reared in tanks (two for each thesis) under the same environmental and dietary conditions. A practical extruded diet (crude protein: 43.4%; ether extract: 19.3%) was offered to fish according to a feeding rate that varied from 1% to 1.5% of live weight. Both at the beginning and at the end of the trials, samples of fish were submitted to morphometrics and chemical analysis. The final body weight and the specific growth rate of triploid fish were significantly lower than those of fish diploid. The feed efficiency of triploids showed a reduction in crude protein retention. Juvenile triploids evidenced a larger amount of coelomatic fat, and their gonads were atrophic. The whole-body proximate composition of 3n fish was characterized by a higher ether extract and a lower crude protein content than 2n fish. Triploids had higher liver lipid content; there were no significant differences in viscera proximate composition. Sum, the results showed that chromosome set manipulation in this species can reduce productive performances at a juvenile stage, influencing some morphometric traits

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Section: Triploidy Induction and Ploidy Determinationmentioning

confidence: 99%

Effects of triploidy on feed efficiency, morphometric indexes and chemical composition of shi drum, Umbrina cirrosa L.

et al. 2006

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“…Although spontaneous reproduction occurs easily in captive seabass breeders (Devauchelle & Coves, 1988), and the preliminary results on sperm characterization and management (Billard, 1984) have been exploited to establish artificial insemination routines (Zanuy et al, 1994;Felip et al, 1997), artificial insemination is still unpredictable (Colombo et al, 1995). The present development of seabass aquaculture and the increase in genetic studies in this species, require reliable artificial insemination and gamete management protocols including sperm economy and storage since males do not provide large volumes of semen (Zohar et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of sperm of captive seabass in relation to its fertilization potential

Fauvel

Savoye

Dréanno

et al. 1999

Journal of Fish Biology

111

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Seabass Dicentrarchus labrax sperm concentration was high (up to 60 10 9 spz ml 1 ) but decreased significantly at the end of the reproductive season (mid-March) in monthly sampled fish. The spermiation period may be shortened by frequent stripping. Sperm can be prediluted up to 1 : 128 in non-activating medium without loss of initial motility and motility duration. Immediately after activation by transfer to sea water, all the spermatozoa were motile for 10 s and then the number of motile cells decreased progressively but sharply to zero, so that the duration of sperm motility was very short (40 s). As a consequence, the fertility of seabass sperm decreased exponentially after 10 s following sperm activation and was zero by 1 min. The sperm requirements for optimal fertilization were c. 66 000 spermatozoa per egg. Scalingup of the experimental insemination procedure yielded better fertilization rates while conserving the individual differences due to the breeder pairs. 1999 The Fisheries Society of the British Isles

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“…Les tétraploïdes sont obtenus par un traitement des oeufs au moment de la première mitose pour bloquer la cytodiérèse aboutissant à la première division cellulaire. Les premiers essais de triploïdisation ont été réalisés, chez le bar, à la fin des années 1990 (Colombo et al, 1995 ;Felip et al, 1997) et des traitements optimaux (par la pression ou par le froid) permettent aujourd'hui la production de 100 % de triploïdes (Peruzzi et Chatain, 2000). Les oeufs doivent être traités, 2 min après la fécondation, à une pression de 800 psi ou immergés pendant 20 min dans de l'eau de mer à 0°C.…”

Section: Amé Lioration Par Le Contrô Le De La Maturationunclassified

La génétique du bar (Dicentrarchus labrax L.)

Chatain¹,

Chavanne²

2009

Agriculture

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La gé né tique du bar (Dicentrarchus labrax L.) Ré suméLa production de bar, espèce majeure de la pisciculture méditerranéenne, repose encore, dans bien des cas, sur des animaux issus de parents sauvages. Pourtant, l'amélioration génétique est une des clés du développement de toutes les filières d'élevage optimisées. Trois types d'amélioration peuvent être réalisés lorsqu'il s'agit de poissons : i) la production de populations monosexes. Pour le bar, ce serait du monosexe femelle, car les femelles sont plus grosses que les mâles (gain de croissance potentielle de 20-30 %). Cependant, le déterminisme du sexe chez cette espèce est très complexe, car il fait intervenir des composantes génétiques et environnementales. Le monosexage femelle n'est donc pas encore maîtrisé en routine, même si on peut parfois obtenir des lots presque entièrement femelles ; ii) les manipulations chromosomiques, au premier rang desquelles l'induction de triploïdes qui aboutit à des animaux viables mais stériles. Cela devrait permettre d'éviter les ralentissements de croissance pour les poissons de plus de 600 g et permettre la protection du progrès génétique. L'induction triploïde est maîtrisée chez le bar, mais n'est pas utilisée, car elle provoque des retards de croissance ; iii) la sélection, qui montre sur la plupart des caractères testés des possibilités de gains importants, probablement du fait que l'espèce n'est actuellement pas encore domestiquée. La « boîte à outils » de l'amélioration génétique est donc disponible chez le bar et les premiers programmes de sélection réellement optimisés sont aujourd'hui en cours de développement.Mots clés : amélioration génétique ; Dicentrarchus labrax ; génétique des populations ; pisciculture ; poisson de mer ; zone méditerranéenne. Thèmes : productions animales ; pêche et aquaculture ; amélioration génétique. Abstract Genetics of European seabass (Dicentrarchus labrax L.)The production of sea bass, a major species in Mediterranean fish farming, still relies in many cases on the production of fingerlings issued from wild breeders. Still, genetic improvement is a major key to sustainable development for all optimized forms of animal production. Three types of genetic improvement can be acheived in fish: i) the production of monosex populations. For seabass, monosex female populations would be the objective as females grow 20-30% faster than males. However, sex determination in this species is complex, impacted by both genetic and environmental factors. Hence, production of monosex populations is not yet a routine operation, although in some cases almost 100% female batches have been obtained; ii) chromosome set manipulations, the most important being triploid induction, which produces viable but sterile fish. The aim in seabass would be to increase late growth and protect genetic gain. Although triploid induction is mastered in seabass, it is not used as it induces some growth retardation; iii) selective breeding, which promises important productivity gains on almost all tested traits, probably d...

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Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass, Dicentrarchus labrax L.

Cited by 32 publications

References 12 publications

Effects of triploidy on feed efficiency, morphometric indexes and chemical composition of shi drum, Umbrina cirrosa L.

Effects of triploidy on feed efficiency, morphometric indexes and chemical composition of shi drum, Umbrina cirrosa L.

Characteristics of sperm of captive seabass in relation to its fertilization potential

La génétique du bar (Dicentrarchus labrax L.)

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