Artificial methods of dentine caries induction: A hardness and morphological comparative study

Marquezan, Marcela; Corrêa, Fernanda Nahás Pires; Sanabe, Mariane Emi; Filho, Leonardo Elóy Rodrigues; Hebling, Josimeri; Guedes-Pinto, Antônio Carlos; Mendes, Fausto Medeiros

doi:10.1016/j.archoralbio.2009.09.007

Cited by 137 publications

(155 citation statements)

References 26 publications

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“…However, when natural lesions are used, it is difficult to standardize all of the variables that occur among samples 20 . It was also difficult to collect the large number of decayed teeth needed 9 . The use of a pHcycling method for inducing artificial caries lesions in human teeth is a good and reliable choice for simulating a carious lesion when compared with other methods, since the hardness values obtained with this cycling model are similar to those obtained from natural caries lesions 9 .…”

Section: Discussionmentioning

confidence: 99%

“…It was also difficult to collect the large number of decayed teeth needed 9 . The use of a pHcycling method for inducing artificial caries lesions in human teeth is a good and reliable choice for simulating a carious lesion when compared with other methods, since the hardness values obtained with this cycling model are similar to those obtained from natural caries lesions 9 . Moreover, among in vitro protocols, this type of pH-cycling involves the combinations of demineralization and remineralization 21 , as the development of caries lesions represents the imbalance in the cycle of demineralization and remineralization 22 .…”

Section: Discussionmentioning

confidence: 99%

“…A modified pH-cycling model was used to simulate the cariogenic challenge and to create artificial dentin carious lesions 9,11 . The demineralizing solution contained 2 mM Ca, 0.075 M acetic acid, and 2 mM PO 4 , with the pH adjusted to 4.35 with HCl.…”

Section: Methodsmentioning

confidence: 99%

“…For this measurement, a microhardness tester (Future-Tech FM Future-Tech Corporation, Kawasaki, Kanagawa, Japan) was used with a Knoop indenter using a 10 g static load applied for 10 sec 15 . Thirty-six indentations were made, divided into three columns that were 100 µm apart at depths of 10, 20, 30, 40, 50, 60, 80, 100, 120, 140, 180, and 200 µm from the bottom of the cavity floor towards the pulp 9 . For each specimen at each dentin level from the pulp floor, a value was obtained by calculating the mean between the three measurements.…”

Section: Methodsmentioning

confidence: 99%

“…A study model is important to simulate the process of caries, and although there has been progress of in situ and in vivo research in Cariology, laboratory tests are still widely used to evaluate dental caries [9][10][11] . Among these models, those mimicking in vitro caries processes (pH-cycling) 11 , are important to reproduce the dynamics of mineral loss and gain involved in caries formation 12 .…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of different removal methods of artificially demineralized dentin

et al. 2016

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Objective: This study aimed to evaluate the effectiveness of different mechanical methods for the removal of demineralized dentin. Methods: Healthy human third molars were prepared in such a way that the flat occlusal surfaces of the dentin were exposed and longitudinally sectioned in a vestibular-lingual direction. One section of each tooth was submitted to the pH-cycling model, while the other section was kept intact. The tooth sections were joined, and a single operator performed dentin removal using a steel bur, a hand instrument, or a polymer bur. The tooth's sections were then separated, and digital images were obtained. The depth of the prepared cavities and the microhardness measurements were checked and analyzed statistically using the Kruskal-Wallis One-Way ANOVA in Ranks, the Tukey test, while desmineralized dentin were compared using the One-Way ANOVA, and the Holm-Sidak method (p < 0.05). Results: The steel bur produced the deepest cavities in mineralized and demineralized dentin. The polymer bur generated the shallowest cavities in demineralized dentin. The measurements of microhardness of the deepest surfaces of the cavities prepared in demineralized dentin indicated that the steel bur and hand instrument presented similar values, while those values produced by polymer burs proved to be lower. Conclusion: The effectiveness of the removal of demineralized dentin varied among the three methods used in this study. The polymer bur proved to be the most conservative of the methods used. By contrast, the steel bur and hand instrument showed a similar effectiveness in the removal of dentin, according to the microhardness of the remaining dentin, even though they produced different cavity depths.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effectiveness of different removal methods of artificially demineralized dentin

et al. 2016

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The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

et al. 2022

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Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross‐linkers have been employed to maintain the structure of collagen; these include epigallocatechin‐3‐gallate (EGCG), which can fortify the mechanical properties of collagen and withstand the degradation of collagenase. We hypothesized that EGCG combined with nHAp may promote resin–dentin bonding durability. Here, we examined the effect of epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica (EGCG@nHAp@MSN) on thermal stability and remineralization capability of dentin collagen. Dentin slices (2 × 2 × 1 mm3) were obtained and completely demineralized in a 10% phosphoric acid water solution. The resulting dentin collagen matrix was incubated with deionized water, EGCG, nHAp@MSN, and EGCG@nHAp@MSN. The collagen thermal degradation temperature was assessed utilizing differential scanning calorimetry analysis, which indicated that EGCG, nHAp@MSN, and EGCG@nHAp@MSN reinforced collagen's capability to resist thermal degradation. EGCG@nHAp@MSN resulted in the highest increase in denaturation temperature. Thermogravimetric analysis showed that both nHAp@MSN and EGCG@nHAp@MSN achieved a higher residual mass than the EGCG and control groups. Fourier transform infrared spectroscopy was performed to examine the interaction between EGCG@nHAp@MSN and dentin collagen. The EGCG@nHAp@MSN sample exhibited stronger dentin microhardness and uppermost bond strength after thermocycling. EGCG significantly enhanced collagen's capability to resist thermal degradation. In summary, EGCG and nHAp@MSN may work together to assist the exposed collagen to improve resistance to thermal cycling and promote remineralization while also strengthening the durability of resin–dentin bonds.

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Assessment of Physical Properties of Dental Restorative Materials

2021

Manual of Laboratory Testing Methods for Dental Restorative Materials

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Artificial methods of dentine caries induction: A hardness and morphological comparative study

Abstract: pH-cycling is more appropriated to simulate a substrate that resembles affected caries dentine layer, after caries removal. The microbiological method seems more indicated to simulate a dentine caries lesion with an infected layer, previously to caries removal.

Cited by 137 publications

References 26 publications

Effectiveness of different removal methods of artificially demineralized dentin

Effectiveness of different removal methods of artificially demineralized dentin

The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

Assessment of Physical Properties of Dental Restorative Materials

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