Artificial wounds implication for the development of mango (<i>Mangifera Indica</i> L. Anacardiaceae) fruit disease caused by <i>Colletotrichum gloeosporioïdes</i> (Penz.) Sacc. (Glomerellaceae)

Abstract

Anthracnose is the most important post-harvest disease of mango caused by Colletotrichum gloeosporioïdes in Côte d'Ivoire. This study was conducted to evaluate the pathogenicity of 5 isolates (CA1, CA2, CB2, CB3 and CK2) of C. gloeosporioïdes. The isolates were obtained from naturally infected fruits of varieties including Brooks (CB2 and CB3), Kent (CK2) and Amelia (CA1 and CA2). The mycelium plugs of each purified isolate were used for a wound or no wound inoculation of Brooks and Keitt mango fruits. The dates of the first lesions appearance with their sizes were assessed. The inoculation without wounds didn't produce any lesion on both varieties. On the other hand, with the wounded method, all the isolates caused lesions on the varieties studied. The first lesions were induced on the 6 th day after inoculation (DAI) on both varieties by isolate CA2. It also produced the largest lesion size on Keitt (3.19 ± 0.39 cm) and Brooks (2.61 ± 0.34 cm). On the opposite, isolate CA1 induced lesions lately with an average at 10.75 and 8.50 DAI, as well as a lower average size of 0.12 ± 0.07 cm and 0.62 ± 0.21 cm, respectively on Brooks and Keitt varieties. Isolates CA2 was the most virulent on the two varieties.

Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia

“…The fruits were washed under tap water and surface‐disinfected with 1% sodium hypochlorite for 5 min, and then rinsed with sterilized distilled water for three times and blot‐dried using sterilized filter paper (Kouame et al . ). Tissue segments containing symptomatic and asymptomatic area were cut with a sterilized scalpel and transferred onto potato dextrose agar with streptomycin.…”

“…Single spores of the Fusarium isolates were grown on PDA and incubated for 5 days at 28 ± 2°C in 12/12‐h dark and light photoperiod (Kouame et al . ). The grown mycelia of Fusarium isolate were cut into small plugs using 5 mm diameter of cork borer (Kouame et al .…”

“…The grown mycelia of Fusarium isolate were cut into small plugs using 5 mm diameter of cork borer (Kouame et al . ). The pathogenicity test of isolated Fusarium species was performed under laboratory conditions.…”

Effect of Thermotherapy on the Development of Anthracnose on Post-harvest Mangoes of the Amelie Variety in Côte d’Ivoire

“…The fungal material is an isolate (CA2) of Colletotrichum gloeosporioïdes (Penz). obtained from a mango symptomatic of anthracnose [10].…”

Pre and postharvest assessment of mango anthracnose incidence and severity in the north of Côte d’Ivoire

“…However, this disease could probably exist for a long time. Further studies have shown that it remains the most important cause of postharvest mango losses in the country (Kouamé et al, 2010;N'Guettia et al, 2014).…”