Anthracnose is the most important post-harvest disease of mango caused by Colletotrichum gloeosporioïdes in Côte d'Ivoire. This study was conducted to evaluate the pathogenicity of 5 isolates (CA1, CA2, CB2, CB3 and CK2) of C. gloeosporioïdes. The isolates were obtained from naturally infected fruits of varieties including Brooks (CB2 and CB3), Kent (CK2) and Amelia (CA1 and CA2). The mycelium plugs of each purified isolate were used for a wound or no wound inoculation of Brooks and Keitt mango fruits. The dates of the first lesions appearance with their sizes were assessed. The inoculation without wounds didn't produce any lesion on both varieties. On the other hand, with the wounded method, all the isolates caused lesions on the varieties studied. The first lesions were induced on the 6 th day after inoculation (DAI) on both varieties by isolate CA2. It also produced the largest lesion size on Keitt (3.19 ± 0.39 cm) and Brooks (2.61 ± 0.34 cm). On the opposite, isolate CA1 induced lesions lately with an average at 10.75 and 8.50 DAI, as well as a lower average size of 0.12 ± 0.07 cm and 0.62 ± 0.21 cm, respectively on Brooks and Keitt varieties. Isolates CA2 was the most virulent on the two varieties.