Arylsulfatase B Mediates the Sulfonation-Transport Interplay in Human Embryonic Kidney 293 Cells Overexpressing Sulfotransferase 1A3

Zhao, Min; Wang, Shuai; Li, Feng; Dong, Dong; Wu, Baojian

doi:10.1124/dmd.116.070938

Cited by 9 publications

(9 citation statements)

References 42 publications

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“…Luciferase Reporter Cotransfection Assays. Cotransfection assays were performed in HEK293 or HepG2 cells using the HET Transfection Kit (Biowit Technologies) as described previously (Zhao et al, 2016). Ligands were added at 16-20 hours post-transfection.…”

Section: Regulation Of Cyp7a1 By the Rev-erba/lrh-1 Axismentioning

confidence: 99%

REV-ERBα Regulates CYP7A1 Through Repression of Liver Receptor Homolog-1

Zhang

Zhao²,

Lu³

et al. 2017

Drug Metab Dispos

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Nuclear heme receptor reverse erythroblastosis virus (REV-ERB) (a transcriptional repressor) is known to regulate cholesterol 7-hydroxylase (CYP7A1) and bile acid synthesis. However, the mechanism for REV-ERB regulation of CYP7A1 remains elusive. Here, we investigate the role of LRH-1 in REV-ERB regulation of CYP7A1 and cholesterol metabolism. We first characterized the tertiary amine -(4-chloro-2-methylbenzyl)--(4-chlorobenzyl)-1-(5-nitrothiophen-2-yl)methanamine (GSK2945) as a highly specific Rev-erb/REV-ERB antagonist using cell-based assays and confirmed expression of Rev-erb in mouse liver. GSK2945 treatment increased hepatic mouse cholesterol 7-hydroxylase (Cyp7a1) level and lowered plasma cholesterol in wild-type mice. Likewise, the compound increased the expression and microsomal activity of Cyp7a1 in hypercholesterolemic mice. This coincided with reduced plasma and liver cholesterol and enhanced production of bile acids. Increased levels of Cyp7a1/CYP7A1 were also found in mouse and human primary hepatocytes after GSK2945 treatment. In these experiments, we observed parallel increases in Lrh-1/LRH-1 (a known hepatic activator of Cyp7a1/CYP7A1) mRNA and protein. Luciferase reporter, mobility shift, and chromatin immunoprecipitation assays revealed that Lrh-1/LRH-1 was a direct Rev-erb/REV-ERB target gene. Furthermore, conditional deletion of Lrh-1 in the liver abrogated the regulatory effects of Rev-erb on Cyp7a1 and cholesterol metabolism in mice. In conclusion, Rev-erb regulates Cyp7a1 and cholesterol metabolism through its repression of the Lrh-1 receptor. Targeting the REV-ERB/LRH-1 axis may represent a novel approach for management of cholesterol-related diseases.

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Section: Regulation Of Cyp7a1 By the Rev-erba/lrh-1 Axismentioning

confidence: 99%

REV-ERBα Regulates CYP7A1 Through Repression of Liver Receptor Homolog-1

Zhang

Zhao²,

Lu³

et al. 2017

Drug Metab Dispos

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“…Glycosaminoglycans are produced by some pathogenic bacteria as extracellular capsule and used in the process of host infection and colonization to facilitate pathogen attachment, invasion and/or evasion of host defensive mechanisms [ 63 ]. Arylsulfatases B can also participate in processes of biotransformation, mediating the sulfonation of xenobiotics by other enzymes to facilitate their excretion [ 64 ]. In this case, increase in the arylsulfatase could be related to the psyllid physiological needs to metabolize the high levels of flavonoids (hesperidin, narirutin and dydimin) produced in citrus plants infected by Ca.…”

Section: Discussionmentioning

confidence: 99%

The molecular interplay of the establishment of an infection – gene expression of Diaphorina citri gut and Candidatus Liberibacter asiaticus

et al. 2021

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Background Candidatus Liberibacter asiaticus (CLas) is one the causative agents of greening disease in citrus, an unccurable, devastating disease of citrus worldwide. CLas is vectored by Diaphorina citri, and the understanding of the molecular interplay between vector and pathogen will provide additional basis for the development and implementation of successful management strategies. We focused in the molecular interplay occurring in the gut of the vector, a major barrier for CLas invasion and colonization. Results We investigated the differential expression of vector and CLas genes by analyzing a de novo reference metatranscriptome of the gut of adult psyllids fed of CLas-infected and healthy citrus plants for 1-2, 3-4 and 5-6 days. CLas regulates the immune response of the vector affecting the production of reactive species of oxygen and nitrogen, and the production of antimicrobial peptides. Moreover, CLas overexpressed peroxiredoxin, probably in a protective manner. The major transcript involved in immune expression was related to melanization, a CLIP-domain serine protease we believe participates in the wounding of epithelial cells damaged during infection, which is supported by the down-regulation of pangolin. We also detected that CLas modulates the gut peristalsis of psyllids through the down-regulation of titin, reducing the elimination of CLas with faeces. The up-regulation of the neuromodulator arylalkylamine N-acetyltransferase implies CLas also interferes with the double brain-gut communication circuitry of the vector. CLas colonizes the gut by expressing two Type IVb pilin flp genes and several chaperones that can also function as adhesins. We hypothesized biofilm formation occurs by the expression of the cold shock protein of CLas. Conclusions The thorough detailed analysis of the transcritome of Ca. L. asiaticus and of D. citri at different time points of their interaction in the gut tissues of the host led to the identification of several host genes targeted for regulation by L. asiaticus, but also bacterial genes coding for potential effector proteins. The identified targets and effector proteins are potential targets for the development of new management strategies directed to interfere with the successful utilization of the psyllid vector by this pathogen.

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“…48 Besides, a developed HEK293 cell overexpressing SULT1A3 (or other SULT isozymes) model can be a simple and practical tool to investigate excretion of bavachinin-O-sulfate and sulfation-transport interplay. 49,50 Further investigations are needed to answer these question.…”

Section: Discussionmentioning

confidence: 99%