Ascorbic Acid and Salicylic Acid Mitigate NaCl Stress in Caralluma tuberculata Calli

Rehman, Riaz Ur; Zia, Muhammad; Abbasi, Bilal Haider; Lü, Gang; Chaudhary, Muhammad Fayyaz

doi:10.1007/s12010-014-0890-6

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…The reason for this phenomenon corresponds to the role of plastoglobuli. It has been proved that plastoglobuli play an active role in metabolic and stress-response pathways, and are a metabolic intersection between different plastid compartments [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

The carbonate concentration mechanism of Pyropia yezoensis (Rhodophyta): evidence from transcriptomics and biochemical data

et al. 2020

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Background Pyropia yezoensis (Rhodophyta) is widely cultivated in East Asia and plays important economic, ecological and research roles. Although inorganic carbon utilization of P. yezoensis has been investigated from a physiological aspect, the carbon concentration mechanism (CCM) of P. yezoensis remains unclear. To explore the CCM of P. yezoensis, especially during its different life stages, we tracked changes in the transcriptome, photosynthetic efficiency and in key enzyme activities under different inorganic carbon concentrations. Results Photosynthetic efficiency demonstrated that sporophytes were more sensitive to low carbon (LC) than gametophytes, with increased photosynthesis rate during both life stages under high carbon (HC) compared to normal carbon (NC) conditions. The amount of starch and number of plastoglobuli in cells corresponded with the growth reaction to different inorganic carbon (Ci) concentrations. We constructed 18 cDNA libraries from 18 samples (three biological replicates per Ci treatment at two life cycles stages) and sequenced these using the Illumina platform. De novo assembly generated 182,564 unigenes, including approximately 275 unigenes related to CCM. Most genes encoding internal carbonic anhydrase (CA) and bicarbonate transporters involved in the biophysical CCM pathway were induced under LC in comparison with NC, with transcript abundance of some PyCAs in gametophytes typically higher than that in sporophytes. We identified all key genes participating in the C4 pathway and showed that their RNA abundances changed with varying Ci conditions. High decarboxylating activity of PEPCKase and low PEPCase activity were observed in P. yezoensis. Activities of other key enzymes involved in the C4-like pathway were higher under HC than under the other two conditions. Pyruvate carboxylase (PYC) showed higher carboxylation activity than PEPC under these Ci conditions. Isocitrate lyase (ICL) showed high activity, but the activity of malate synthase (MS) was very low. Conclusion We elucidated the CCM of P. yezoensis from transcriptome and enzyme activity levels. All results indicated at least two types of CCM in P. yezoensis, one involving CA and an anion exchanger (transporter), and a second, C4-like pathway belonging to the PEPCK subtype. PYC may play the main carboxylation role in this C4-like pathway, which functions in both the sporophyte and gametophyte life cycles.

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Section: Discussionmentioning

confidence: 99%

The carbonate concentration mechanism of Pyropia yezoensis (Rhodophyta): evidence from transcriptomics and biochemical data

et al. 2020

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“…Supplemental AsA application effectively lowered the oxidative stress in salt-stressed Phaseolus vulgaris , as indicated by the reduction of malondialdehyde (MDA) and ROS accumulation through activating their immune systems related with up-regulation of SOD, CAT and GR activities [187]. The AsA recovered salinity-induced oxidative damage in Caralluma tuberculata by lowering the activity of APX, POD, CAT, and GR, which were increased upon saline toxicity [188]. Exogenous AsA-induced plant tolerance, especially on the AsA-GSH pathway, is cultivar dependent [189].…”

Section: Exogenous Use Of Asa and Gsh In Conferring Abiotic Stressmentioning

confidence: 99%

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

et al. 2019

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Reactive oxygen species (ROS) generation is a usual phenomenon in a plant both under a normal and stressed condition. However, under unfavorable or adverse conditions, ROS production exceeds the capacity of the antioxidant defense system. Both non-enzymatic and enzymatic components of the antioxidant defense system either detoxify or scavenge ROS and mitigate their deleterious effects. The Ascorbate-Glutathione (AsA-GSH) pathway, also known as Asada–Halliwell pathway comprises of AsA, GSH, and four enzymes viz. ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, play a vital role in detoxifying ROS. Apart from ROS detoxification, they also interact with other defense systems in plants and protect the plants from various abiotic stress-induced damages. Several plant studies revealed that the upregulation or overexpression of AsA-GSH pathway enzymes and the enhancement of the AsA and GSH levels conferred plants better tolerance to abiotic stresses by reducing the ROS. In this review, we summarize the recent progress of the research on AsA-GSH pathway in terms of oxidative stress tolerance in plants. We also focus on the defense mechanisms as well as molecular interactions.

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“…The plant (C. tuberculata) material was obtained from Quetta (Balochistan, Pakistan) and is identified by taxonomist Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan. The methodology to produce callus is adopted as described by Rehman et al (2014b). Briefly, the stem portions are treated with water, following 0.2% Triton X 100 and finally with bevistin.…”

Section: Plant Materials and Explant Preparationmentioning

confidence: 99%

Salicylic acid and ascorbic acid retrieve activity of antioxidative enzymes and structure of Caralluma tuberculata calli on PEG stress

Rehman¹,

Zia²,

Chaudhary³

2017

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Biochemical adaptations and morphological changes are cellular aptitude originated on biotic and abiotic stresses. Polyethylene glycol (PEG) induces drought stress in the nutrient solution. In the present investigation, Caralluma tuberculata calli is exposed to PEG and antioxidative molecules. By increasing the level of antioxidative enzymes (SOD, POD, CAT, APX, and GR), the PEG-stressed calli falls off upon exposure to non-enzymatic antioxidants (ascorbic acid and salicylic acid). Under PEG-stress, several cellular and sub-cellular changes such as alteration in plasma membrane thickness, change in nucleus shape, increase in nucleoli, deformation of thylakoid membranes, and increase in plastoglobuli are observed through electron microscopic images. From our results we conclude that application of PEG (a drought causative agent) leads to an increase in the level of antioxidative enzymes and also deformation of cellular organelles. However, application of ascorbic acid and salicylic acid eradicate drought effect induced by PEG.

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Ascorbic Acid and Salicylic Acid Mitigate NaCl Stress in Caralluma tuberculata Calli

Cited by 8 publications

References 45 publications

The carbonate concentration mechanism of Pyropia yezoensis (Rhodophyta): evidence from transcriptomics and biochemical data

The carbonate concentration mechanism of Pyropia yezoensis (Rhodophyta): evidence from transcriptomics and biochemical data

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Salicylic acid and ascorbic acid retrieve activity of antioxidative enzymes and structure of Caralluma tuberculata calli on PEG stress

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