Ascorbic acid prevents cellular uptake and improves biocompatibility of chitosan nanoparticles

Elshoky, Hisham A.; Salaheldin, Taher A.; Ali, Maha A.; Gaber, Mohamed H.

doi:10.1016/j.ijbiomac.2018.04.055

Cited by 21 publications

(6 citation statements)

References 24 publications

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“…Worth noting here is the absence of the absorption bands at 1686 cm −1 (carboxyl moiety of FA [38]) and 2018 cm −1 (isothiocyanate moiety (N=C=S) of the FITC [39]), together with the appearance of the absorption band (ca. 1650 cm −1 ) assigned to the amide thioamide bonds [42], that confirm the covalent link between CS and FA [43], and CS and FITC [44], respectively, in agreement with data reported elsewhere [45]. The morphology and size of the CNCs nanosystems functionalized with the FITC derivative were assessed by STEM (Figure 3).…”

Section: Preparation and Characterization Of The Cncs/fa-cs-fitc Nanosystemssupporting

confidence: 88%

Cellulose Nanocrystals/Chitosan-Based Nanosystems: Synthesis, Characterization, and Cellular Uptake on Breast Cancer Cells

et al. 2021

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Cellulose nanocrystals (CNCs) are elongated biobased nanostructures with unique characteristics that can be explored as nanosystems in cancer treatment. Herein, the synthesis, characterization, and cellular uptake on folate receptor (FR)-positive breast cancer cells of nanosystems based on CNCs and a chitosan (CS) derivative are investigated. The physical adsorption of the CS derivative, containing a targeting ligand (folic acid, FA) and an imaging agent (fluorescein isothiocyanate, FITC), on the surface of the CNCs was studied as an eco-friendly methodology to functionalize CNCs. The fluorescent CNCs/FA-CS-FITC nanosystems with a rod-like morphology showed good stability in simulated physiological and non-physiological conditions and non-cytotoxicity towards MDA-MB-231 breast cancer cells. These functionalized CNCs presented a concentration-dependent cellular internalization with a 5-fold increase in the fluorescence intensity for the nanosystem with the higher FA content. Furthermore, the exometabolic profile of the MDA-MB-231 cells exposed to the CNCs/FA-CS-FITC nanosystems disclosed a moderate impact on the cells’ metabolic activity, limited to decreased choline uptake and increased acetate release, which implies an anti-proliferative effect. The overall results demonstrate that the CNCs/FA-CS-FITC nanosystems, prepared by an eco-friendly approach, have a high affinity towards FR-positive cancer cells and thus might be applied as nanocarriers with imaging properties for active targeted therapy.

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Section: Preparation and Characterization Of The Cncs/fa-cs-fitc Nanosystemssupporting

confidence: 88%

Cellulose Nanocrystals/Chitosan-Based Nanosystems: Synthesis, Characterization, and Cellular Uptake on Breast Cancer Cells

et al. 2021

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“…In addition, poor chemical stability and lower cellular entry lead to the available concentration of <1 mM at intra-/extracellular space, even at the highest dosage tested. Thus, research is directed to achieve the effective therapeutic property at a lower dose of vitamin C. In particular, the development of nanomaterial encapsulated delivery systems has been focused in the past few years. − These nanoscale systems include liposomal nanoparticle, chitosan nanoparticle, mesoporous silica, glyceryl behenate, polymer nanoparticle, , and liposome . In these nanoparticle forms, the chemical stability of vitamin C is enhanced against oxidation along with the enhanced cell delivery efficiency.…”

Section: Therapeutic Application Of Nano-vitamin C With Enhanced Pro-...mentioning

confidence: 99%

Enhanced Therapeutic Applications of Vitamin C via Nanotechnology-Based Pro-Oxidant Properties: A Review

Pal

Jana

2022

ACS Appl. Nano Mater.

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Vitamin C has a controversial history in terms of its antioxidant as well as pro-oxidant properties. While the antioxidant property of vitamin C is an established fact, the pro-oxidant property is less known. This is particularly because the pro-oxidant property of vitamin C occurs only under selected conditions that include high concentration and/or the presence of a catalytic metal. Recent works show that the nanoformulation of vitamin C or the coupling with a nanoparticle can significantly enhance the pro-oxidant property of vitamin C. This Review has focused on the current status of the utilization of the pro-oxidant property of vitamin C in various therapeutic applications. In particular, the design of the nanoparticle form of vitamin C and the other nanotechnology approach to enhance the pro-oxidant property of vitamin C have been reviewed. We discuss the chemical origin of the antioxidant or the pro-oxidant property of vitamin C under different conditions and highlight the advantages of the nanoparticle form of vitamin C (or nano-vitamin C) in enhancing the performance. It has been shown that nanotechnology approaches offer an enhanced cell delivery of vitamin C with enhanced intracellular Fenton reaction-based oxidative stress and Ferroptosis therapy. As a result, the pro-oxidant property of vitamin C can now be utilized at a physiologically relevant vitamin C concentration that was not possible earlier. We expect that nanotechnology approaches of enhancing the pro-oxidant property of vitamin C would emerge as a powerful therapeutic approach.

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“…CNPs were prepared at the Central Nanotechnology Characterization Lab, Agriculture Research Center, Ministry of Agriculture, Cairo, Egypt. The ionotropic gelation method was used as a described in a previous study [8] with modifications. First, a solution of 50 ml of 0.2% chitosan solution (2mg/ ml) was prepared by dissolving CS (Acros Organics, Belgium) in 2% (v/v) acetic acid solution (Sigma Aldrich, USA) in deionized water (Millipore-Sigma, Burlington, Massachusetts, USA) under magnetic stirring (Cimarec, Thermo Scientific, USA).…”

Section: Preparation Of Cnps Irrigationmentioning

confidence: 99%

Assessment of the Effect of Chitosan Nanoparticles on the Ultra-Structure of Dentinal Wall: A Comparative In vitroStudy

Soliman¹,

Roshdy²,

Lutfy³

2020

Act Scie Dental

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This study was conducted to evaluate the efficacy of 0.2% Chitosan Nanoparticles (CNPs), CNPs/EDTA (1:1) and 17% EDTA on smear layer removal ability.Methods: Fifteen extracted human permanent mandibular premolars with single canals were decoronated and biomechanically prepared using protaper next rotary system and 2.5% Sodium hypochlorite, then randomly distributed according to the final irrigation protocol into 3 group; Group I (0.2% CNPs), Group II (CNPs/EDTA) (1:1), and Group III (17% EDTA). A standardized volume of 5 ml of each tested irrigant (s) was used for 3 minutes. Teeth were longitudinally split to evaluate the smear layer eradication using scanning electron microscope at magnifications of 500x and 1000x. The micrographs for each third were given scores by two blinded observers using Takeda., et al. (1999) scoring system.Results: There was no statistically significant difference in the scores of the three groups at the coronal, middle, and apical thirds (Pvalue = 0.368, 0.054, and 0.0900) respectively. As for the combined total scores of each group, Group I (CNPs) showing a significantly higher smear layer elimination than both Group II (CNPs/EDTA) and Group III (EDTA) (P-value = 0.044).Conclusions: None of the used chelating agents resulted in complete removal of the smear layer. The combined use of CNPs/EDTA (1:1) did not show superior performance over the other two groups.

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Ascorbic acid prevents cellular uptake and improves biocompatibility of chitosan nanoparticles

Cited by 21 publications

References 24 publications

Cellulose Nanocrystals/Chitosan-Based Nanosystems: Synthesis, Characterization, and Cellular Uptake on Breast Cancer Cells

Cellulose Nanocrystals/Chitosan-Based Nanosystems: Synthesis, Characterization, and Cellular Uptake on Breast Cancer Cells

Enhanced Therapeutic Applications of Vitamin C via Nanotechnology-Based Pro-Oxidant Properties: A Review

Assessment of the Effect of Chitosan Nanoparticles on the Ultra-Structure of Dentinal Wall: A Comparative In vitroStudy

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