Ascorbic acid responsive genes during neuronal differentiation of embryonic stem cells

Shin, Dong‐Mi; Ahn, Joon-Ik; Lee, Ki-Hwan; Lee, Yong-Sung; Lee, Yeon-Sook

doi:10.1097/00001756-200408260-00025

Cited by 63 publications

(48 citation statements)

References 24 publications

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“…Previous reports showed that ascorbic acid can influence proliferation and differentiation of CNS progenitor cells both in vivo and in vitro [27,32,42,55,70]. Our data appear to be consistent with these reports.…”

Section: Discussionsupporting

confidence: 93%

Elevated dopamine levels during gestation produce region-specific decreases in neurogenesis and subtle deficits in neuronal numbers

2007

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Dopamine levels in the fetal brain were increased by administering the dopamine precursor 3,4-dihydroxy-L-phenylalanine (L-DOPA) to pregnant mice in drinking water. The L-DOPA exposure decreased bromodeoxyuridine (BrdU) labeling in the lateral ganglionic eminence and frontal cortical neuroepithelium but not medial or caudal ganglionic eminences. The regional differences appear to reflect heterogeneity in precursor cells' responses to dopamine receptor activation. Relative numbers of E15 generated neurons were decreased at postnatal day 21 (P21) in the caudate-putamen, nucleus accumbens and frontal cortex but not globus pallidus in the L-DOPA group. TUNEL labeling did not show significant differences on P0, P7 or P14 in the caudate-putamen or frontal cortex, suggesting that cell death was not altered. Although virtually all cells in the P21 brains that were labeled with the E15 BrdU injection were NeuN-positive, stereological analyses showed no significant changes in total numbers of NeuN-positive or NeuN-negative cells in the P21 caudate-putamen or frontal cortex. Thus persisting deficits in neuronal numbers were evident in the L-DOPA group only by birth-dating analyses and not upon gross histological examination of brain sections or analysis of total numbers of neurons or glia. One explanation for this apparent discrepancy is that L-DOPA exposure decreased cell proliferation at E15 but not at E13. By E15, expansion of the neuroepithelial precursor pool is complete and any decrease in cell proliferation likely produces only marginal decreases in the total numbers of cells generated. Our L-DOPA exposure model may be pertinent to investigations of neurological dysfunction produced by developmental dopamine imbalance.

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Section: Discussionsupporting

confidence: 93%

Elevated dopamine levels during gestation produce region-specific decreases in neurogenesis and subtle deficits in neuronal numbers

2007

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“…An aliquot of RNA (500 ng) was reverse-transcribed into cDNA by oligo(dT) [12][13][14][15][16][17][18] primer. Real time PCR contained 100 ng of template cDNA, 1ϫ SYBR GREEN master mix (Qiagen), and 100 nM specific forward and reverse primers in a 25-l volume of reaction.…”

Section: Methodsmentioning

confidence: 99%

“…The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18vincing evidence that AA also influences differentiation of other cell type lineages, such as neuronal cells, that produce little or no matrix (16). Furthermore, the gulonolactone oxidase knock-out mice have normal levels of proline hydroxylation and collagen production in the tail, mammary gland, and tumors, whereas these mice show widespread abnormalities in multiple organs besides the bone (2,17).…”

mentioning

confidence: 87%

Nuclear Factor-E2-related Factor-1 Mediates Ascorbic Acid Induction of Osterix Expression via Interaction with Antioxidant-Responsive Element in Bone Cells

Xing

Singgih

Kapoor

et al. 2007

Journal of Biological Chemistry

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We recently found that deletion of the gulonolactone oxidase gene, which is involved in the synthesis of ascorbic acid (AA), was responsible for the fracture phenotype in spontaneous fracture mice. To explore the molecular mechanisms by which AA regulates osteoblast differentiation, we examined the effect of AA on osterix expression via Nrf1 (NF-E2-related factor-1) binding to antioxidant-responsive element (ARE) in bone marrow stromal (BMS) cells. AA treatment caused a 6-fold increase in osterix expression in mutant BMS cells at 24 h, which was unaffected by pretreatment with protein synthesis inhibitor. Sequence analyses of mouse osterix promoter revealed a putative ARE located at ؊1762 to ؊1733 upstream of the transcription start site to which Nrf potentially binds. A gel mobility shift assay revealed that nuclear proteins from AA-treated BMS cells bound to radiolabeled ARE much more strongly than nuclear extracts from AA-untreated cells. A chromatin immunoprecipitation assay with Nrf1 antibody confirmed the interaction of Nrf1 with the mouse osterix promoter.

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“…[36][37][38] AsA has been reported to induce differentiation of embryonic cortical precursors and midbrain neuroblasts into neurons and to alter gene expression involved in neurogenesis, maturation, and neurotransmission. [39][40][41][42] Our present study indicates the possibility that AsA may regulate the redox state in the neural cells and play a crucial role in the differentiation and development of the brain. However, mechanism of neuronal differentiation regulated by AsA is not yet clear.…”

Section: Discussionmentioning

confidence: 53%

Essential Role of Ascorbic Acid in Neural Differentiation and Development: High Levels of Ascorbic Acid 2-Glucoside Effectively Enhance Nerve Growth Factor-Induced Neurite Formation and Elongation in PC12 Cells

Haramoto

Tatemoto

Muto

2008

JOURNAL OF HEALTH SCIENCE

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The aim of this study was to investigate the role of ascorbic acid (AsA) in neural differentiation induced by nerve growth factor (NGF) in PC12 cells, a neural precursor cell line. Cells were cultivated in the presence of L-ascorbic acid-2-O-α-D-glucopyranoside (AA-2G) and evaluated for potentiation of neural differentiation induced by a small amount of NGF. AA-2G enhanced the proportion of neurite-bearing cells in a concentration-dependent manner (<1 mM). When cultivated with relatively high concentrations of AA-2G (1-5 mM), the cells tended to extend neurites further and to form a neuron-like network. The resultant number of neurite-bearing cells was apparently increased by the addition of AA-2G, with a concurrent decrease in the number of total cells in the same culture dish. These results suggest that AsA in the brain not only promotes NGF-induced differentiation of neural precursor cells but also participates in the development of neural network-forming cells.

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Ascorbic acid responsive genes during neuronal differentiation of embryonic stem cells

Cited by 63 publications

References 24 publications

Elevated dopamine levels during gestation produce region-specific decreases in neurogenesis and subtle deficits in neuronal numbers

Elevated dopamine levels during gestation produce region-specific decreases in neurogenesis and subtle deficits in neuronal numbers

Nuclear Factor-E2-related Factor-1 Mediates Ascorbic Acid Induction of Osterix Expression via Interaction with Antioxidant-Responsive Element in Bone Cells

Essential Role of Ascorbic Acid in Neural Differentiation and Development: High Levels of Ascorbic Acid 2-Glucoside Effectively Enhance Nerve Growth Factor-Induced Neurite Formation and Elongation in PC12 Cells

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