Ascospore germination, growth in culture, and imperfect spore formation in Cookeina sulcipes and Phillipsia crispata

Abstract

PADEN, J. W. 1975. Ascospore germination, growth in culture, and imperfect spore formation in Cookeina slrlcipes and Phillipsia crispata. Can. J. Bot. 53: 56-61. Ascospores of Cookeina sulcipes germinate by one of two modes: (1) by the production of blastoconidia on sympodially proliferating conidiogenous cells which may arise from any point on the spore surface, and (2) by a thick polar germ tube. No ascospores were seen to germinate both ways. The conidiogenous cells are occasionally modified into narrow hyphae. The blastoconidia germinate readily but are evidently very short-lived. Ascospores of Phillipsia crispata germinate by two polar germ tubes; there is no formation of blastoconidia. In both species the inner ascospore wall separated froman outer wall layer duringgermination. In culture both C . sulcipes and P. crispata form arthroconidia. The arthroconidia are uninucleate; they germinate readily and reproduce the species when transferred to fresh plates. PADEN, J. W. 1975. Ascospore germination, growth in culture, and imperfect spore formation in Cookeina sulcipes and Phillipsia crispata. Can. J. Bot. 53: 56-61. Les ascospores de Cookeina sulcipes germent selon l'un de deux modes: (1) par la production de blastoconidies sur des cellules conidiogenes qui prolifirent de manikre sympodiale et qui peuvent se former n'importe oh sur la surface de la spore; ou (2) par la formation d'un Cpais tube germinatif polaire. L'auteur n'a vu aucune ascospore germer de ces deux f a~o n s . Les cellules conidiogenes sont occasionnellement modifiCes en hyphes Ctroits. Les blastoconidies germent facilement mais ont une durCe de vie trks brkve. Les ascospores de Phillipsia crispata germent par deux tubes germinatifs polaires; il ne se forme pas de blastoconidies. Chez les deux espkces, la paroi interne de I'ascospore se sCpare d'une paroi externe au cours de lagermination. Cookeina sulcipes e t P. crispata forment tous deux des arthroconidies en culture. Les arthroconidies sont uninuclCCes; elles germent facilement et reproduisent I'espkce lorsqu'elles sont placCes dans des plats de culture frais.[Traduit par le journal]There is at present little information on ascoAscospores were harvested by suspending fragments of spore germination, cultural or im-apothecia above 2% Difco Noble agar containing aureomycin + streptomycin (2.5/250 ppm). The ascospores of perfect states Pezizales. BOedijn both species germinated readily. Cultures were obtained 1932) described ascospore germination in cook-by transferring hyphal tips from germinated spores t o einasuIcipes (Berk.) 0. Kuntze and C . tricholoma potato carrot agarslants (Dade 1960) containing aureo-(Mont.) 0. Kuntze but stated that he did not mycin $-obtain cultures. Pfister (1973) germinated the Observations ascospores of Nanoscypha tetraspora (Seav. in J . Stev. ex Seav.) Denison and cultured this species. Paden (1974) germinated the ascospores of Phillipsia domingensis (Berk.) Berk. and P. Iutea Denison and reported on their cultural characters. Ascospore germination, growth ...

<i>Microstoma longipilum</i> sp. nov. (<i>Sarcoscyphaceae, Pezizales</i>) from Japan

“…The direct germination of ascospores to produce mitospores in Sarcoscyphaceae have been reported in some species of Cookeina Kuntze (Boedijn, 1929;Boedijn, 1933;Paden, 1975), Nanoscypha Denison (Pfister, 1973), and Sarcoscypha (Fr.) Boud.…”

“…( Rosinski, 1953 ; Baral, 1984 ; Harrington, 1990 ; Fenwick, 1994 ). In conidia production, while blastoconidia production on germ tubes is widely known in such genera ( Boedijn, 1933 ; Rosinski, 1953 ; Pfister, 1973 ; Paden, 1975 ; Baral, 1984 ; Harrington, 1990 ; Fenwick, 1994 ), direct production of conidiogenous cells on ascospores is restricted to Cookeina sulcipes (Berk.) Kuntze ( Boedijn, 1929 ; Boedijn, 1933 ; Paden, 1975 ).…”

“…In conidia production, while blastoconidia production on germ tubes is widely known in such genera ( Boedijn, 1933 ; Rosinski, 1953 ; Pfister, 1973 ; Paden, 1975 ; Baral, 1984 ; Harrington, 1990 ; Fenwick, 1994 ), direct production of conidiogenous cells on ascospores is restricted to Cookeina sulcipes (Berk.) Kuntze ( Boedijn, 1929 ; Boedijn, 1933 ; Paden, 1975 ). Microstoma longipilum is the second report of the latter type of ascospore germination producing mitospores in Sarcoscyphaceae .…”

Octospora conidiophora (Pyronemataceae) – a new species from South Africa and the first report of anamorph in bryophilous Pezizales

“…(Davidson 1950), Cookeina Kuntze, Phillipsia Berk. (Paden 1975), Pithya Fuckel (Paden 1972), Nanoscypha Denison (Pfister 1973), Sarcoscypha (Fr.) Boud.…”

Ascomycete Systematics

“…We should expect this type of conidiogenesis to have arisen independently several times. In fact, arthroconidia are found rather sporadically throughout both the ascomycetes (Onygenales, Pezizales; Paden, 1975) and basidiomycetes (Tsuneda et al, 1993).…”