Asebotin, a dihydrochalcone glucoside from Guibourtia tessmannii

Nkengfack, Augustin Ephram; Heerden, Fanie R. van; Fuendjiep, Victorine; Fomum, Zacharias Tanee

doi:10.1016/s0367-326x(01)00300-8

Cited by 10 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plants recorded are known to have beneficial properties arguing for the treatment of several ailments ( Table 2). Phytochemical studies of the plant extract revealed the presence of many bioactive compounds such as tannins, flavonols and sugars which have been previously described 14,15 . The results of the preliminary pharmacological investigations showed that the methanolic extract of Guibourtia tessmannii (EMGT) exerted vasorelaxant effect in the isolated aorta pre-contracted with KCl or with adrenalin.…”

Section: Discussionmentioning

confidence: 90%

“…It appeared clearly, in accordance with NGUESSAN KOFFI 4 that the use of mono-specific receipts would be less dangerous and more appropriate for the health of large populations. 11,2,12 , and Guibourtia tessmannii 13,14 . Among all species recorded and well documented for their traditional use in the management of hypertension, G. tessmannii was the most used species.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

2012

IJPSR

View full text Add to dashboard Cite

This work was conducted at a Libreville herbal market located in Peyrie in order to inventory plants used by people for the management of cardiovascular diseases such as hypertension and to evaluate their pharmacological effects. The method of preparation and modes of administration were also recorded. Twenty nine herbalists were interviewed using questionnaires. Twenty two plant species belonging to sixteen families and seventeen recipes were identified. The commonly used plants were Guibourtia tessmannii, Musanga ceropioiodes, Senecio gabonensis. Among them, G. tessmannii appeared to be the most used plant species. Phytochemical studies on extracts of G. tessmannii revealed the presence of alkaloids, sugars, polyphenols, sterols, tannins and saponosids. Pharmacological studies performed in the isolated aorta of rats showed a vasorelaxant effect on adrenalin-or KCl-induced contraction. G Tessmanniiinduced vasorelaxation was significantly but not totally reduced by endothelium removal or by a pretreatment with L-NAME, suggesting the involvement of endothelium-dependent and -independent mechanisms. Medicinal plants and G. tessmannii in particular may represent a source of efficient antihypertensive agents. INTRODUCTION:Hypertension was for a long time considered as a disease affecting only people in developed countries. However, nowadays, it represents a public health problem for developing countries in Africa, especially in Gabon. According to WHO estimations, in 2000, 26.4 % of the world population (26.6 % male and 26.1 % female) was affected by hypertension and developing countries represent the most affected part of the world.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Untitled

2012

IJPSR

View full text Add to dashboard Cite

show abstract

“…The mass spectra were recorded across the range of 50–1500 m/z (Scan rate: 1.00 Hz) with automatic gain control to provide high accuracy mass measurements within 0.40 ppm deviation using Na formate as calibrant (Fofié, Nguelefack‐Mbuyo, Tsabang, Kamanyi, & Nguelefack, ). Identification of the compounds present in the methanolic extract of G. tessmannii was based on the information from the MS spectral and the data present in the literature (Nkengfack et al., ; Roux & DE‐Bruyn, ; Wan‐Yi et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…The phytochemical analysis of G. tessmannii confirmed the presence of terpenoids, flavonoids, phenols and tannins (Mbaveng et al., ; Nyangono, Tsague, Ngondi, & Oben‐Julius, ; Watcho et al., ). Additionally, various bioactive compounds such as asebotin (a dihydrochalcone glucoside) have been identified and isolated from the stem barks of G. tessmannii (Nkengfack, Van, Fuendjiep, & Fomum, ). Previous studies reported the antioxidant properties of asebotin towards the 2,2‐diphenyl‐1‐picrylhydrazyl radical (Cuendet, Potterat, Salvi, Testa, & Hostettmanna, ).…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, TRPV1 is a Ca 2+ permeable channel gated by noxious heat, oxidative stress and capsaicin (CAP), and inhibited by capsazepine (CPZ) (Nazıroğlu, Dikici, & Dursun, ; Nazıroğlu et al., ). Although many studies reported the antioxidant effects of selenium (Jones & Radi, ; Oberley et al., ) and some bioactive compounds isolated from G. tessmannii (Cuendet et al., ; Nkengfack et al., ), no works on the protective effect of selenium and G. tessmannii against cytotoxicity in the Leydig cells have been conducted. In this study, we aimed to determine the effects of the methanolic extract of G. tessmannii and selenium on cell viability, intracellular free Ca 2+ level ([Ca 2+ ] i ), apoptosis and oxidative stress in R2C tumour Leydig cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The methanolic extract ofGuibourtia tessmannii(caesalpiniaceae) and selenium modulate cytosolic calcium accumulation, apoptosis and oxidative stress in R2C tumour Leydig cells: Involvement ofTRPV1 channels

et al. 2018

View full text Add to dashboard Cite

This study evaluated the effects of the methanolic extract of Guibourtia tessmannii (GT) and selenium (Se) on cell viability, intracellular calcium concentration ([Ca2+]i), apoptosis and oxidative stress through transient receptor potential vanilloid 1 (TRPV1) channel activity in CCL‐97 (R2C) tumour Leydig cells. The cells were divided into nine groups and treated as follows: (a)‐Control, (b)‐Capsazepine (CPZ, 0.1 mM, a TRPV1 channel blocker), (c)‐Capsaicin (CAP, 0.01 mM, a TRPV1 channel activator), (d)‐GT (500 μg/ml), (e)‐GT+CPZ, (f)‐GT+CAP, (g)‐Se (200 nM), (h)‐Se+CPZ and (i)‐Se+CAP. After treatments, cell viability, [Ca2+]i, apoptosis, caspase 3/9, reactive oxygen species (ROS) and mitochondrial membrane depolarisation (MMD) were evaluated. The [Ca2+]i, apoptosis, caspase 3/9, MMD and ROS levels were significantly (p < 0.001) increased in CAP group, but lowered in CPZ group. Interestingly, these parameters were significantly (p < 0.001) improved by GT and Se, compared to the CAP group. Moreover, the co‐administration of GT+CAP or Se+CAP inhibited the cytotoxicity of CAP. Thus, the modulatory properties of GT and Se on Ca2+ influx, apoptosis and oxidative stress require the integrity of TRPV1 channel in CCL‐97 Leydig cells. These results suggest that GT and Se might be used in the management of cytotoxicity in the testes, involving TRPV1 channel activity.

show abstract

Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities

Mbaveng¹,

Kuete²,

Mapunya³

et al. 2011

Environmental Toxicology and Pharmacology

View full text Add to dashboard Cite

Methanol extracts from the leaves, bark and roots of four Cameroonian medicinal plants, Bersama engleriana, Cupressus lusitanica, Vitellaria paradoxa and Guibourtia tessmannii were tested for their in vitro cytotoxicity, antigonorrheal and antireverse transcriptase activities. The XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt) assay, the dilution method and reverse transcriptase (RT) assay were used for the investigations. Preliminary phytochemical analysis of the extracts was also conducted using standrad methods. Results showed that all extracts contained compounds belonging to the classes of phenols and terpenoids. They were also able to reduce in dose dependent manner, the proliferation of the cancer THP-1, DU145, HeLa, MCF-7, HepG2 and the normal Vero cells. IC 50 values below 30 µg/ml were noted with extract from the three parts of B. engleriana on at least two of the five studied cancer cell lines, the lowest value of 5.9 µg/ml being obtained with sample from the bark. IC 50 values below 30 µg/ml were also recorded with extracts from the leaves (on HeLa cells) and bark (on MCF-7) of G. tessmanii, and that from the bark of C. lusitanica on MCF-7. Extracts from B. engleriana and those from 2 the bark of V. paradoxa gave the minimal inhibitory concentrations (MIC) values below 100 μg/ml on most of the ten tested Nesseria gonorrhoeae strains. Extracts from B. engleriana also inhibited more than 80% the activity of the Human Immuno-deficiency Virus (HIV) enzyme. Finally, the results of the present study provide baseline information for the use of B. engleriana, C. lusitanica, G. tessmanii, V. paradoxa.

show abstract

Asebotin, a dihydrochalcone glucoside from Guibourtia tessmannii

Cited by 10 publications

References 5 publications

Untitled

Untitled

The methanolic extract ofGuibourtia tessmannii(caesalpiniaceae) and selenium modulate cytosolic calcium accumulation, apoptosis and oxidative stress in R2C tumour Leydig cells: Involvement ofTRPV1 channels

Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities

Contact Info

Product

Resources

About