Aspartic acid racemization in teeth from Ketef Hinnom, a late Holocene burial cave in Jerusalem: Evidence for reuse

Sinibaldi, M.; Goodfriend, Glenn A.; Barkay, Gabriel; Marchese, Stefano; Gil‐Av, E.

doi:10.1002/(sici)1520-6548(199906)14:5<441::aid-gea5>3.0.co;2-0

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…Some factors, such as pH, ionic strength, metal ions presence and water content, have a direct effect on racemization rates (Bada 1972). The variability in racemization ratios in samples from the same site has been interpreted as a consequence of the existence of individual differences in age at death (Sinibaldi et al 1999), differences in response to the extraction procedure (Carolan et al 1997), and local differences in time of formation of cave layers (Torres et al 2002).…”

Section: Previous Research On Modern Samples Have Reported That Variomentioning

confidence: 99%

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Fernández

Ortíz

Torres

et al. 2008

Forensic Science International: Genetics Supplement Series

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The extent of racemization of aspartic acid (Asp) -expressed as D/L ratio-has been used as a marker of biomolecular degradation in ancient remains. However, Asp racemization rate is highly variable, and depends on biochemical and geochemical factors. In this paper we aim to determine to which extent the fraction analyzed and the Results show significant differences between the D/L Asp ratios obtained in dentine and whole tooth fractions from the same tooth and individual, as well as among dentine samples from the same archaeological site and among samples of the same age from different, though nearby, archaeological sites. Ancient DNA (aDNA) could be characterized in the majority of the samples, independently from their racemization values, which suggests that other factors, apart from the sample preservation stage, are more significantly affecting the racemization rate. A generalized use of the Asp racemization ratio as a threshold value for ancient DNA preservation needs to be questioned until further methodological standardization is considered.

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Section: Previous Research On Modern Samples Have Reported That Variomentioning

confidence: 99%

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Fernández

Ortíz

Torres

et al. 2008

Forensic Science International: Genetics Supplement Series

View full text Add to dashboard Cite

show abstract

“…Some factors, such as pH, ionic strength, metal ions presence and water content, have a direct effect on racemization rates (Bada, 1972). The variability in racemization ratios in samples from the same site has been interpreted as a consequence of the existence of individual differences in age at death (Sinibaldi et al, 1999), differences in response to the extraction procedure (Carolan et al, 1997), and local differences in time of formation of cave layers (Torres et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Aspartic acid racemization variability in ancient human remains: implications in the prediction of ancient DNA recovery

Fernández¹,

Ortíz²,

Pérez‐Pérez³

et al. 2009

Journal of Archaeological Science

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Geochemical evolution of amino acids in dentine of pleistocene bears

Torres¹,

Ortíz²,

García³

et al. 2001

Chirality

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A linear correlation was established between aspartic acid racemization ratio from cave bear dentine collagen and absolute dating. The high correlation coefficient obtained allowed age calculation through amino acid racemization. Aspartic acid and glutamic acid racemization kinetics have also been explored in dentine from a North American black bear (Ursus americanus Pallas). Three sample sets were prepared for kinetic heating experiments in nitrogen atmosphere: one water soaked, one with a water-saturated nitrogen atmosphere, and one without any moisture. It was possible to show that the presence of water is a factor controlling amino acid racemization rate. The aspartic acid in a heating experiment at 105 degrees C shows an "apparent kinetics reversal" which can be explained by a progressive hydrolysis of amino acid chains (proteins and polypeptides). Because of the low potential of collagen preservation over long periods of time, the apparent kinetics reversal phenomenon will not affect the dating of old material where no traces of collagen remain. An apparent kinetics reversal was not observed in glutamic acid, which racemizates more slowly.

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Aspartic acid racemization in teeth from Ketef Hinnom, a late Holocene burial cave in Jerusalem: Evidence for reuse

Cited by 6 publications

References 16 publications

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Mitochondrial DNA genetic relationships at the ancient Neolithic site of Tell Halula

Aspartic acid racemization variability in ancient human remains: implications in the prediction of ancient DNA recovery

Geochemical evolution of amino acids in dentine of pleistocene bears

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