Aspects of extraction, spiking and distribution in the determination of incurred residues of chloramphenicol in animal tissues

Abstract

Studies of distribution, extraction procedures and spiking protocols in the determination of incurred chloramphenicol residues in animal tissues have been carried out. An extraction procedure involving glucuronidase enzyme digestion was found to extract 10 times more incurred chloramphenicol from pig kidney than direct extraction without digestion. However, neither protease digestion nor ultrasonic probe treatment resulted in improved chloramphenicol extraction. Chloramphenicol was found to be inhomogeneously distributed within kidney from a treated pig. Highest concentrations were detected in the renal medulla. Muscle tissues from the same animal were found to contain a lower concentration of chloramphenicol residues, but no chloramphenicol residues were detectable in the liver. Chloramphenicol recovery from spiked pig liver was found to be lower than that from kidney, but was improved by the addition of piperonyl butoxide before extraction. This additive had no effect on recovery from spiked pig or cattle kidney. The implications of these results for regulatory surveillance of animal tissue for chloramphenicol residues are discussed.

Study of the distribution and depletion of chloramphenicol residues in bee products extracted from treated honeybee (Apis melliferaL.) colonies

“…This situation contrasts to mammalian systems where up to 90% of the excretory forms of CAP are CAP N-acetyl conjugate (Cooper et al, 1998).…”

Liquid chromatography/tandem mass spectrometry analysis of chloramphenicol in propolis extracts available on the Italian market

“…Several reports have described estimation of CAP by liquid chromatography-tandem mass spectrometry (LC/MS/MS) in shrimp (Neuhaus et al, 2002;Rønning et al, 2006), meat (Bogusz et al, 2004;Cooper et al, 1998;Li et al, 2002;Mottier et al, 2003;Rønning et al, 2006), milk (Guy et al, 2004;Huang et al, 2006;Rønning et al, 2006), and honey (Ashwin et al, 2005;Forti et al, 2005;Huang et al, 2006;Ortelli et al, 2004;Rønning et al, 2006;Turnipseed et al, 2002;Vanhoenacker et al, 2003;Verzegnassi et al, 2003;Vivekanandan et al, 2005).…”

Ultrasound assisted extraction in quantifying lutein from chicken liver using high-performance liquid chromatography☆

“…Ultrasound assisted extraction has been used in analyzing trace organic compounds in soil, animal, and plant tissues, and food packaging materials [10][11][12][13][14][15][16][17][18]. Those studies demonstrated that ultrasound could increase the extraction yield of targeted compounds in sample preparation.…”