Liquid chromatography/tandem mass spectrometry analysis of chloramphenicol in propolis extracts available on the Italian market

Bononi, M.; Tateo, F.

doi:10.1016/j.jfca.2007.08.003

Cited by 17 publications

(5 citation statements)

References 16 publications

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“…Methods for honey and associated products (such as propolis and bee pollen) typically involve extraction of honey diluted in water with ethyl acetate (Bononi and Tateo, 2008;Taka et al, 2012;Douny et al, 2013), acetonitrile or acetonitrile/salt solution (Rønning et al, 2006;Cronly et al, 2010a), or methanol/1 % metaphosphoric acid (Fujita et al, 2008). Some methods describe direct extraction and clean-up of chloramphenicol from honey with C 18 SPE (Bogusz et al, 2004) or use of C 18 SPE for further clean-up of an ethyl acetate extract (Ortelli et al, 2004) or of a dichloromethane/acetone extract (Forti et al, 2005) of the honey samples.…”

Section: Confirmatory Methodsmentioning

confidence: 99%

Scientific Opinion on Chloramphenicol in food and feed

2014

EFS2

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Chloramphenicol is an antibiotic not authorised for use in food-producing animals in the European Union (EU). However, being produced by soil bacteria, it may occur in plants. The European Commission asked EFSA for a scientific opinion on the risks to human and animal health related to the presence of chloramphenicol in food and feed and whether a reference point for action (RPA) of 0.3 µg/kg is adequate to protect public and animal health. Data on occurrence of chloramphenicol in food extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF) were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which chloramphenicol is present at 0.3 µg/kg in all foods of animal origin, foods containing enzyme preparations and foods which may be contaminated naturally. The mean chronic dietary exposure for this worst-case scenario would range from 11 to 17 and 2.2 to 4.0 ng/kg b.w. per day for toddlers and adults, respectively. The potential dietary exposure of livestock to chloramphenicol was estimated to be below 1 µg/kg b.w. per day. Chloramphenicol is implicated in the generation of aplastic anaemia in humans and causes reproductive/hepatotoxic effects in animals. Margins of exposure for these effects were calculated at 2.4 × 10 5 or greater and the CONTAM Panel concluded that it is unlikely that exposure to food contaminated with chloramphenicol at or below 0.3 µg/kg is a health concern for aplastic anaemia or reproductive/hepatotoxic effects. Chloramphenicol exhibits genotoxicity but, owing to the lack of data, the risk of carcinogenicity cannot be assessed. The SUMMARYChloramphenicol is a broad-spectrum antibiotic effective against Gram-positive and Gram-negative bacteria and, in the past, has been widely used to treat infections in both humans and animals. Chloramphenicol is not authorised for use in food-producing animals in the European Union (EU) but may be used in human medicine and in treatments for non-food-producing animals. Apart from its potential occurrence as a residue in food from illicit treatment of food-producing animals, chloramphenicol has also been used in feed and food enzyme products and may occur naturally in plants from its production by the soil bacterium Streptomyces venezuelae.The EFSA Scientific Opinion entitled "Guidance on methodological principles and scientific methods to be taken into account when establishing Reference Points for Action (RPAs) for non-allowed pharmacologically active substances present in food of animal origin" identified an approach for establishing RPAs for various categories of non-allowed pharmacologically active substances. However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances causing blood dyscrasias (aplastic anaemia) such as chloramphenicol. As chloramphenicol is excluded fro...

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Section: Confirmatory Methodsmentioning

confidence: 99%

Scientific Opinion on Chloramphenicol in food and feed

2014

EFS2

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“…In China, the government Ministry of Agriculture proposed to ban the addition of four antibiotics such as norfloxacin and PEF in animal food. At present, the antibiotic detection methods include high‐performance liquid chromatography (Christina vakh et al, 2018), high‐performance liquid chromatography–tandem mass spectrometry (Bononi & Tateo, 2008), capillary electrophoresis (Long,, Deng, Sun, & Meng, 2017), immunoassay (Wang et al, 2017), enzyme linked immunoassay (Mahmoudi, Norian, Pajohi‐Alamoti, 2017), and so forth. However, these methods are usually complex pre‐treatments involved, time‐consuming and expensive.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of typical fluoroquinolone antibiotics in livestock feed using terahertz spectrum

Cao¹,

Zhu³

et al. 2022

J Food Process Engineering

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The antibiotic residues are increasing concerns in agricultural and food products, an effective, rapid, and accurate detection method of antibiotics is still in exploration stage for applications. In this study, we employed terahertz (THz) spectroscopy as a tool to find its feasibility to detect quinolone antibiotics in livestock feed (LSF) quantitatively. A total of 220 samples with pefloxacin (PEF) and fleroxacin (FLE) in LSF were prepared separately and the THz spectrum of each sample was measured using a TERA K15 THz‐TDS system. Then, the absorption characteristics of PEF and FLE in THz band were analyzed. For the binary and ternary mixtures, successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS) algorithm, and the combination algorithm (CARS‐SPA) were selected as dimensionality reduction process methods, and back propagation neural network (BPNN) was selected to establish a model for the quantitative analysis. The results showed, PEF has two typical absorption peaks at 0.775 and 0.988 THz, and FLE has two typical absorption peaks at 0.919 and 1.088 THz, which implies the features can be used for qualitative identification. For the quantitative analysis, The CARS‐BPNN model achieved the best predictive ability based on the prediction set, and the correlation coefficients can reach 0.98 for both the binary and ternary mixtures. The results also showed that predictive models of the ternary mixtures have better performance than that of the binary mixtures. The research provides theoretical and technical reference for practical detection of antibiotic residues in livestock and poultry industry.

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“…They can be present as residues in food [25,27], which may cause allergic reactions or antibiotic resistance phenomena in humans [4,22]. The quinolones mostly used as veterinary antimicrobial drugs are the fluoroquinolones [6,7,8,20]. Maximum residue limits (MRLs) have been established in different animal feed matrices for eight quinolones: marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, flumequine and oxolinic acid [2,5,11,30].…”

Section: Introductionmentioning

confidence: 99%

Estimation of Quinolones, Ceftiofur, and Thiamphenicol Residues Levels in Honey

Morariu¹

2021

FARMACIA

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Quinolones, cephalosporins and amphenicols are antimicrobial agents effective in treating many infectious diseases caused by bacteria in both human and veterinary medicine. However, its residues in food products leads to increased bacterial resistance. Therefore, the quantification and limitation of such medicines should be verified through fast and reliable methods. The aim of the research was the simultaneous quantitative determination of quinolones, ceftiofur and thiamphenicol using biochip technology. The biochip method had excellent accuracy. The decision limit obtained for the analysed drugs was between 1.25 and 4.5 µg/kg. The detection capacity recorded values in the range of 2.05 -8.51 µg/kg for the analysed drugs. The recovery coefficient obtained was in the range of 77 -123%. The results of the analysis of Romanian honey samples using the biochip method were confirmed using a validated LC-MS/MS method. RezumatChinonolele, cefalosporinele și amfenicolii sunt agenți antimicrobieni eficienți în tratarea a numeroase boli infecțioase cauzate de bacterii atât în medicina umană, cât și în cea veterinară. Totuși, reziduurile acestor compuși în alimente conduce în timp la o rezistență bacetriană crescută. Prin urmare, cuantificarea și limitarea acestor medicamente ar trebui verificate prin metode rapide și fiabile. Scopul cercetărilor a fost determinarea cantitativă simultană a chinolonelor, ceftiofurului și tiamfenicolului folosind tehnologia biochip. Metoda biochip a avut o precizie excelentă. Limita de decizie obținută pentru substanțele analizate s-a încadrat între valorile 1,25 și 4,5 µg/kg. Capacitatea de detecție a înregistrat valori pentru chimioterapicele determinate în intervalul 2,05 -8,51 µg/kg. Coeficientul de recuperare obținut s-a situat în domeniul 77 -123%. Rezultatele analizei probelor de miere românească folosind metoda biochip au fost confirmate printr-o metodă LC-MS/MS validată.

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Liquid chromatography/tandem mass spectrometry analysis of chloramphenicol in propolis extracts available on the Italian market

Cited by 17 publications

References 16 publications

Scientific Opinion on Chloramphenicol in food and feed

Scientific Opinion on Chloramphenicol in food and feed

Quantitative analysis of typical fluoroquinolone antibiotics in livestock feed using terahertz spectrum

Estimation of Quinolones, Ceftiofur, and Thiamphenicol Residues Levels in Honey

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