Aspects of microbial quality of some milk products in Abuja Nigeria

Okpalugo, J; Ibrahim, K.; Izebe, KS; Inyang, US

doi:10.4314/tjpr.v7i4.14703

Cited by 20 publications

(22 citation statements)

References 14 publications

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“…This may likely be due to observance of simple hygiene during collection. The findings of this present studies agree with the reports of earlier researchers who reported the presence of pathogenic bacteria in cow milk in Czech Republic, cow foremilk in Botswana, pasteurized milk and soya milk in Nigeria (Adeleke et al, 2000;Schlegelova et al, 2002;Guta et al, 2002;Okpalugo et al, 2008).…”

Section: Discussionsupporting

confidence: 93%

“…Salmonella is the most frequent pathogen associated with outbreaks of foodborne illnesses (80.5% of the outbreaks), followed by Escherichia coli (10.1%) (De Buyser et al, 2001). Cooked food products and raw milk were most commonly contaminated with food borne pathogens and many of them were resistant to different antibiotics (Walsh et al, 2005;Normanno et al, 2007;Okpalugo et al, 2008 andNovakova et al, 2010). Contamination of dairy foods with virulent pathogens renders them to be a source of public health hazard.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic and molecular characterization of Salmonella serotypes in cow raw milk and milk products in Nigeria

Olufunke¹,

Abike²,

Oriade³

2014

Afr. J. Biotechnol.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Phenotypic and molecular characterization of Salmonella serotypes in cow raw milk and milk products in Nigeria

Olufunke¹,

Abike²,

Oriade³

2014

Afr. J. Biotechnol.

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“…Multiple resistance patterns were observed in penicillin-ampicillin (9.3%) and erythromycin, tetracycline and ampicillin at 3.6%. Ahmed et al (2001) and Okpalugo et al (2008) also reported high antibiotic resistance to tetracycline and other antibiotics by organisms isolated from milk and milk products. In the present study, only one of the isolates (Bacillus subtilis) from brand J was resistant to three classes of antibiotics namely tetracyclines, aminoglycosides and βetalactams.…”

Section: Discussionmentioning

confidence: 99%

“…The relatively high level of resistance to antimicrobial agents constitutes a major threat to public health as it may spread bacterial resistance among the populace who come in contact with the milk products. Since public perception of food quality is critical in the marketing of any product, it is very important that the Nigerian milk products industry maintains high processing standards (Okpalugo et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Assessment of microbiological and residual antibiotics status in milk sold in Abeokuta, Ogun State. Nigeria

Oluwafemi

Oluwabamiwo

Oluwatosin

et al. 2018

Turkish JAF Sci.Tech.

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This study investigated the prevalence and concentration of antibiotic residues in twenty brands of milk. Determination and quantification of antibiotic residues was done using High Performance Liquid Chromatography. Aerobic plate count was carried out and the associated bacteria isolated. Identification of bacteria was done using standard microbiological methods. Molecular characterization and identification of bacterial isolates was done using 16S rRNA gene sequencing method. Antibiotic sensitivity was carried out on bacterial isolates using disc diffusion method. Plasmid profile of drug resistant isolates was done using alkaline analysis method. Comparison of means was done using Analysis of Variance. Antibiotic residues were detected in 10 (50%) of the 20 milk brands analyzed. The residual levels of tetracycline, oxytetracycline and chlortetracycline ranged between 5ng/kg and 1569ng/kg while none of the samples had doxycycline residues. The prevalence of tetracycline residues in evaporated milk brands and powdered milk samples were 100 and 23 % respectively. The aerobic plate counts ranged from 2.5 × 10 2 to 6.5 × 10 2 CFU∕mL for evaporated milk and 2.5 × 10 1 to 6.0 × 10 1 CFU∕mL for powdered milk. A total of ten bacterial species were isolated and identified. Susceptibility result showed that 95% of the isolates were sensitive to erythromycin, cefuroxime and gentamycin; 12.5% were resistant to tetracycline and ampicillin, 10% were resistant to ofloxacin and 7.5% to doxycycline and penicillin. Five of the bacterial isolates were resistant to more than one class of antibiotics. Resistant isolates subjected to plasmid profiling had detectable plasmids with estimated sizes between 120bp and 1000bp. The obtained results provide evidence that the presence of antibiotic residues in evaporated and powdered milk is an indication that the public is exposed to the harmful effects of the residues.

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“…The results obtained were then compared with standard references for proper identification of the isolates; Bergey's manual was used for bacteria identification [5].…”

Section: Identification Of Isolatesmentioning

confidence: 99%