“…Minimum inhibitory concentration (MIC) of the newly synthesized compounds against tested bacteria was determined using macrodilution tube method as recommended by NCCLS [69]. Applying the agar plate diffusion technique [59] some of the newly synthesized compounds were screened in vitro for antimicrobial activity against representative Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis), Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa), yeast, (Candida albicans), and fungi, (Aspergillus niger). In this method a standard 5-mm diameter sterilized filter paper disc impregnated with the compound (0.3 mg/0.1 ml of dimethyl-formamide) was placed on an agar plate seeded with thetested organism.…”