Aspects of the Mechanism of Action of Some Cephalosporins

Russell, A. D.; Fountain, R. H.

doi:10.1128/jb.106.1.65-69.1971

Cited by 20 publications

(20 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In correlation with this occurrence of fimH amongst beta lactamase producers was 100% in the present study. A study conducted in China, hetero-resistance among the biofilm forming A. baumannii strains, was observed with both cephalosporins (cefipime and ceftazidime) and penicillin resistance with 65% and 68.98% against respectively [19,20]. Frequency of fimH among the cycline group of drugs was 20% in the present study.…”

Section: Fig 2 Frequency Of Fimhgene Among Different Groups Of Antibiotic Resistant Strains Ofsupporting

confidence: 52%

Frequency of Adhesive Virulence Factor fimH among the Clinical Isolates of Acinetobacter baumannii in India

Padmaja

Girija

Priyadharsini

2020

JPRI

View full text Add to dashboard Cite

The objective of the study was to detect the presence of fimH gene among the drug resistanst strains of Acinetobacter baumannii. fimH gene was found to be associated with a catch bond mechanism which led to better evolution of biofilm formation. Since there are not many studies done with this gene it would be a timely investigation and this study mainly aims in molecular characterization of fimH gene among clinical isolates of A. baumannii. Semi quantitative bio adherent assay was done by the multidrug resistant strains of A. baumannii to find the formation of biofilm. The DNA was extracted with the help of kit and PCR was performed for amplification. Pearson correlation analysis was done to find the existing correlation between the fimH gene and MDR strains of A. baumannii with significant p-value of (<0.05). From the screened 73 genomes of MDR A. baumannii 6.8% showed positive amplicons for the fimH gene which were related to biofilm and porin formation (Fig. 1). Correlation of its existence was high in beta lactamase (100%), cephems (100%), folate (100%) resistant strains, followed by aminoglycosides (80%), carbapenems (60%) and fluoroquinolones (60%) and efflux pumps (20%). In Spite of various measures undertaken to prevent the disease, the prevalence of the pathogen is multiplying. The current study recorded the presence of fimHgene (6.8%) among the clinical isolates of A.baumannii. This gene can be used as a target to develop new drugs and vaccines to combat the menace of A.baumannii infection.

show abstract

Section: Fig 2 Frequency Of Fimhgene Among Different Groups Of Antibiotic Resistant Strains Ofsupporting

confidence: 52%

Frequency of Adhesive Virulence Factor fimH among the Clinical Isolates of Acinetobacter baumannii in India

Padmaja

Girija

Priyadharsini

2020

JPRI

View full text Add to dashboard Cite

show abstract

“…To track 50S subunits, we fluorescently tagged L1 ribosomal protein and expressed the L1-GFP fusion as the only copy of L1 from the native promoter in an FtsZ-depletion strain. The observations that the L1 protein is incorporated into 50S subunits, with the amount of freely diffusing form being very small (40), that the L1-GFP is functional (28), and that inhibition of cell division does not affect transcription or protein synthesis in bacteria (41,42) suggest that the dynamics of L1-GFP in cell filaments are likely to reflect the normal dynamics of 50S subunits.…”

Section: Fraction Of Ribosomes Engaged In Translationmentioning

confidence: 99%

In Vivo Biochemistry in Bacterial Cells Using FRAP: Insight into the Translation Cycle

Llopis

Sliusarenko

Heinritz

et al. 2012

Biophysical Journal

View full text Add to dashboard Cite

In vivo measurements of the mobility and binding kinetics of cellular components are essential to fully understand the biochemical processes occurring inside cells. Here, we describe a fluorescence recovery after photobleaching-based method that can be easily implemented to the study of reaction-diffusion processes in live bacteria despite their small size. We apply this method to provide new, to our knowledge, quantitative insight into multiple aspects of the bacterial translation cycle by measuring the binding kinetics and the micrometer-scale diffusive properties of the 50S ribosomal subunit in live Caulobacter cells. From our measurements, we infer that 70% of 50S subunits are engaged in translation and display, on average, limited motion on the micrometer scale, consistent with little mixing of transcripts undergoing translation. We also extract the average rate constants for the binding of 50S subunits to 30S initiation complexes during initiation and for their release from mRNAs when translation is completed. From this, we estimate the average time of protein synthesis and the average search time of 50S subunits before they engage in the next initiation event. Additionally, our experiments suggest that so-called free 50S subunits do not diffuse freely; instead their mobility is significantly slowed down, possibly through transient associations with mRNA.

show abstract

“…In fact, the target of penicillin has been demonstrated to be the bacterial cell wall, a unique microbial structure. (A trivial exception to this generalization exists [204]. The cephalosporin 7-(5-benzyl-thioacetamido)-cephem-3-ylmethyl-N-dimethyldithiocarbamate-4-carboxylic acid also inhibits protein synthesis and thus is active against both fungi and bacterial spheroplasts.…”

Section: Introductionmentioning

confidence: 99%

Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

Blumberg¹,

Strominger²

1974

Bacteriological Reviews

183

View full text Add to dashboard Cite

The beta-lactam antibiotics stand out among As discussed below, this ring may be that part antimicrobial agents for-their wide spectrum of of the molecule which reacts with the penicillin activity and for their remarkably low toxicity to target. The side chains attached to the nucleus animals. These substances are composed of two are of importance because of their ease of chemical modification and the profound effects 'Present address: Department of Biology, Massachusetts which they exert on the properties of the mole

show abstract

Aspects of the Mechanism of Action of Some Cephalosporins

Cited by 20 publications

References 14 publications

Frequency of Adhesive Virulence Factor fimH among the Clinical Isolates of Acinetobacter baumannii in India

Frequency of Adhesive Virulence Factor fimH among the Clinical Isolates of Acinetobacter baumannii in India

In Vivo Biochemistry in Bacterial Cells Using FRAP: Insight into the Translation Cycle

Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

Contact Info

Product

Resources

About