Aspirin in retrieving the inactivated catalase to active form: Displacement of one inhibitor with a protective agent

Panahi, Yunes; Yekta, Reza; Dehghan, Gholamreza; Rashtbari, Samaneh; Baradaran, Behzad; Jafari, Nematollah Jonaidi; Moosavi-Movahedi, Ali Akbar

doi:10.1016/j.ijbiomac.2018.10.183

Cited by 8 publications

(2 citation statements)

References 33 publications

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“…The current results were consistent with many previous studies that have been reported the interaction of phytochemical compounds such as Hesperidin and THSG [ 98 , 99 , 100 , 101 , 102 ], as well as drugs such as aspirin, levothyroxine and isoxsuprine hydrochloride with catalase [ 76 , 103 ]. Kinetics studies show that the bovine liver catalase increases in the presence of ellagic acid (ELA) and fluorescence analysis data reveals two binding sites for this compound on the catalase and a static type of quenching mechanism.…”

Section: Resultssupporting

confidence: 93%

“…Therefore, catalase is a potential target in many diseases, including diabetes. Drug designers should protect its catalytic function under different patho-physiological conditions [ 75 , 76 ] because the decrease in catalytic function would cause the accumulation of hydrogen peroxide in vivo, leading to oxidative damage in proteins and nucleic acids [ 77 , 78 ]. For these reasons, we used Lamber W-function to analyze of rCAT activity in our experimental groups.…”

Section: Resultsmentioning

confidence: 99%

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Effects of Moringa oleifera Leaf Extract on Diabetes-Induced Alterations in Paraoxonase 1 and Catalase in Rats Analyzed through Progress Kinetic and Blind Docking

et al. 2020

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In our study, we aimed to evaluate the effects of Moringa oleifera leaves extract on rat paraoxonase 1 (rPON1) and catalase (rCAT) activities in alloxan-induced diabetic rats. Our study included three groups; group C (control, n = 5); group D (diabetic, n = 5); and group DM (M. oleifera extract-supplemented diabetic rats, n = 5). Daily oral administration of M. oleifera extract at 200 mg/kg doses produced an increase in endogenous antioxidants. Serum rPON1 (lactonase) and liver cytosol catalase activities were determined by a spectrophotometric assay using progress curve analysis. We found a decrease in the Vm value of rPON1 in diabetic rats, but dihydrocoumarin (DHC) affinity (Km) was slightly increased. The value of Vm for the DM group was found to be reduced approximately by a factor of 3 compared with those obtained for group C, whereas Km was largely changed (96 times). Catalase activity was significantly higher in the DM group. These data suggest that the activation of rPON1 and rCAT activities by M. oleifera extracts may be mediated via the effect of the specific flavonoids on the enzyme structure. In addition, through molecular blind docking analysis, rPON1 was found to have two binding sites for flavonoids. In contrast, flavonoids bound at four sites in rCAT. In conclusion, the data suggest that compounds from M. oleifera leaves extract were able to influence the catalytic activities of both enzymes to compensate for the changes provoked by diabetes in rats.

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