Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification

Zhan, Yuanbo; He, Zhiwei; Liu, Xinpeng; Miao, Nan; Feng, Lin; Xu, Wenxia; Mu, Sen; Mu, Haibin; Yuan, Mengtong; Cao, Xiaofang; Jin, Han; Liu, Zhongshuang; Li, Ying; Zhang, Bin

doi:10.1016/j.biocel.2018.02.010

Cited by 17 publications

(10 citation statements)

References 69 publications

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“…18 AS may also have clinical potential in the treatment of secondary nociceptive hypersensitivity in OA. 19 These studies suggested that AS may be used for OA prevention and treatment in the future. somes regulated the GPRC5A-YAP signalling axis to improve sulfur mustard-induced acute lung injury.…”

Section: Discussionmentioning

confidence: 99%

Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

et al. 2022

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Bone marrow mesenchymal stem cells (BMMSCs) transplantation methods are promising candidates for osteoarthritis (OA) treatment. However, inflammatory factors (such as TNF-α) that occur at cell transplantation sites are critical factors that impair the effectiveness of the treatment. Previous studies have shown that aspirin (AS) had a regulatory role in stem cell differentiation. However, little is known about the role of AS on the chondrogenesis of BMMSCs. The purpose of this study is to explore the protective role of AS against the negative effects of TNF-α on BMMSC chondrogenesis. In this study, we investigated the effects of AS and TNF-α on BMMSCs chondrogenesis by performing the Alcian Blue staining, safranin O-fast green staining, haematoxylin and eosin staining, and immunohistochemical staining, as well as realtime RT-PCR and western blot assays. Our results demonstrated that TNF-α inhibited chondrogenic differentiation of BMMSCs by disrupting the balance of cartilage metabolism and promoting oxidative stress in BMMSCs, while AS treatment attenuated these effects. Furthermore, a detailed molecular mechanistic analysis indicated that Yes-associated protein (YAP) played a critical regulatory role in this process. In addition, AS treatment mitigated the progression of cartilage degeneration in a mouse destabilization of the medial meniscus (DMM) model. AS alleviated the inhibitory effect of TNF-α on chondrogenesis of BMMSCs by stabilizing YAP, which may provide new therapeutic strategies for OA treatment.

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Section: Discussionmentioning

confidence: 99%

Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

et al. 2022

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“…Acetylation of histones H3 and H4 is functionally coupled with chromatin-remodeling events that mediate the developmental induction of osteocalcin gene during osteoblast differentiation [34]. In contrast, the aspirin (< 100 μg/mL) could reverse the down-regulated histone deacetylases activity and induce inhibition of BMMSCs adipogenesis [35]. Moreover, low-dose aspirin exhibited excellent chemotactic effects in vitro [36].…”

Section: Introductionmentioning

confidence: 99%

Dose-dependent roles of aspirin and other non-steroidal anti-inflammatory drugs in abnormal bone remodeling and skeletal regeneration

et al. 2019

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The failure of remodeling process that constantly regenerates effete, aged bone is highly associated with bone nonunion and degenerative bone diseases. Numerous studies have demonstrated that aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs) activate cytokines and mediators on osteoclasts, osteoblasts and their constituent progenitor cells located around the remodeling area. These cells contribute to a complex metabolic scenario, resulting in degradative or synthetic functions for bone mineral tissues. The spatiotemporal effects of aspirin and NSAIDs in the bone remodeling are controversial according the specific therapeutic doses used for different clinical conditions. Herein, we review in vitro, in vivo, and clinical studies on the dose-dependent roles of aspirin and NSAIDs in bone remodeling. Our results show that low-dose aspirin (< 100 μg/mL), which is widely recommended for prevention of thrombosis, is very likely to be benefit for maintaining bone mass and qualities by activation of osteoblastic bone formation and inhibition of osteoclast activities via cyclooxygenase-independent manner. While, the roles of high-dose aspirin (150–300 μg/mL) and other NSAIDs in bone self-regeneration and fracture-healing process are difficult to elucidate owing to their dual effects on osteoclast activity and bone formation of osteoblast. In conclusion, this study highlighted the potential clinical applications of low-dose aspirin in abnormal bone remodeling as well as the risks of high-dose aspirin and other NSAIDs for relieving pain and anti-inflammation in fractures and orthopedic operations.

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“…It has a certain impact on bone metabolism and bone health, promoting osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), inhibiting adipogenic differentiation of BMSCs, activating osteoblasts and inhibiting osteoclasts. [18][19][20][21][22] It has been found to promote bone formation by inhibiting the expression of inflammatory factors such as IFN-γ and TNF-α. [23][24][25] It may also improve bone marrow microenvironment and enhance immune regulation of BMSCs.…”

mentioning

confidence: 99%

Asprin‐loaded strontium‐containing α‐calcium sulphate hemihydrate/nano‐hydroxyapatite composite promotes regeneration of critical bone defects

Jiang

Qin

Wan

et al. 2020

J Cellular Molecular Medi

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Tumour resection, malformation, sports injury and infection can damage normal musculoskeletal system, leading to bone defects. 1-4 More than 1.5 million patients undergo bone graft surgery every year in the world. 5 Despite significant advances in bone graft techniques in recent years, critical bone defects still pose enormous challenges to their morphological and functional reconstruction which is

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Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification

Cited by 17 publications

References 69 publications

Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP

Dose-dependent roles of aspirin and other non-steroidal anti-inflammatory drugs in abnormal bone remodeling and skeletal regeneration

Asprin‐loaded strontium‐containing α‐calcium sulphate hemihydrate/nano‐hydroxyapatite composite promotes regeneration of critical bone defects

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