Aspirin induces non‐enzymatic formation of platelet‐activating factor from lyso platelet‐activating factor

Abstract

Substantial amounts of platelet-activating factor (PAF) were formed when IysoPAF was mixed with aspirin (e.g. 0.04% of added IysoPAF (200 nmol) was converted to PAF when mixed with aspirin (2 pmol) for 1 h). Non-enzymatic formation of PAF from aspirin and 1ysoPAF also occurs in the aqueous solution or in organic solvents in time-dependent and dose-dependent manners. Possible meanings of the non-enzymatic formation of PAF are discussed.

Non‐enzymatic platelet‐activating factor formation by acetylated proteins

“…Moreover, aspirin is able to acetylate BSA [16,26]. PAF formation observed in the present study by acetylated BSA was several times higher than the one reported resulting from mixing lyso-PAF with aspirin in a buffer [25]. Thus mediation of a protein may enhance chemical PAF formation by a widely used acetylated xenobiotic.…”

“…A chemical acetylation of lyso-PAF by aspirin has been reported [25]. Moreover, aspirin is able to acetylate BSA [16,26].…”