Assay of alterations in oxidative stress markers in pigs naturally infested with Sarcoptes scabiei var. suis

Dimri, Umesh; Bandyopadhyay, S.; Singh, Shanker K.; Ranjan, Rakesh; Mukherjee, Reena; Yatoo, Mohd Iqbal; Patra, Pabitra Hriday; De, Ujjwal Kumar; Dar, Ahsana

doi:10.1016/j.vetpar.2014.06.015

Cited by 16 publications

(3 citation statements)

References 20 publications

(30 reference statements)

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“…Regarding the latter study GSH, SOD, GPx concentrations in blood were significantly declined in the clinical and subclinical sarcoptic mange groups, in contrast to the healthy controls, whereas LPO content of diseased pigs was significantly higher. From the present study, it may be concluded that sarcoptic mange was related to remarkable alterations in the oxidative stress markers, which promptly necessitates correction of the antioxidant status of the infested pigs [29] . In another research thiol-disulphide hemostasis was examined on calves undergoing dehorning process with different analgesia protocols.…”

Section: Discussionmentioning

confidence: 71%

Sarkoptik Uyuzlu Koyunlarda Serum Tiyol Disülfit Seviyeleri

Çamkerten¹,

Çamkerten²,

Erdoğan³

et al. 2019

Kafkas Univ Vet Fak Derg

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Sarcoptic mange, a notable parasitic disease, causes dermatological alterations among ruminants. Thiol-disulphite hemostasis is a novel oxidative stress parameter. The aim of this study was to evaluate dynamic thiol/disulfide homeostasis in sheep with sarcoptic mange. Total of thirty-six sheep (n=15 female, n=21 male) with sarcoptic mange (Group I), and twelve healthy sheep (Group II) were used in the study. A novel method was used to determine the thiol disulphide parameters. Native thiol, total thiol and Disulphide values were statistically lower in Group I. Disulphide/native thiol, Disulphide/total thiol, and Native thiol/ total thiol proportions had no statistical differences in groups. Sarcoptic mange was probably affected by the thiol Disulphide hemostasis in infected sheep. Thus, the data obtained in this study might form base for further studies to include antioxidant molecules in the treatment protocols.

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Section: Discussionmentioning

confidence: 71%

Sarkoptik Uyuzlu Koyunlarda Serum Tiyol Disülfit Seviyeleri

Çamkerten¹,

Çamkerten²,

Erdoğan³

et al. 2019

Kafkas Univ Vet Fak Derg

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“…It is known, that many diseases in sows are linked with oxidative stress (Brambilla et al 2013, Štukelj et al 2013, Dimri et al 2014. It has been also found that oxidative stress effects sows performance (Kim et al 2013, Kim andKim 2017).…”

Section: Introductionmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Szczubiał

2020

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The aim of the present study was to determine changes in oxidative status in plasma of sows during periparturient period using markers of lipid and protein peroxidation intensity. Plasma from 12 pregnant gilts collected on days 14 and 1 before parturition and on days 1, 7 and 14 postpartum was used in the study. As indicators of alterations in the oxidative status, plasma concentrations of thiobarbituric acid reactive substances (TBARS) and sulphydryl groups (SH groups) were measured spectrophotometrically, as well as plasma concentrations of bityrosine and formylokinurenine were determined by the spectrofluorimetric method. Compared to the 14 th day antepartum, the concentration of TBARS increased significantly on day 1 postpartum and then decreased significantly on days 7 and 14 after parturition. The concentration of SH groups increased significantly on the last day before parturition and decreased significantly on day 14 postpartum. The concentration of bityrosine increased significantly on the last day before parturition and decreased significantly on days 7 and 14 postpartum. The concentration of formylokinurenine increased significantly on the last day before parturition, remained at this level on the first day after parturition and then decreased slightly on days 7 and 14 postpartum. These results indicate that during periparturient period in sows, especially around the parturition, the oxidative/antioxidative balance is disturbed, which leads to oxidative stress. Considering that oxidative stress is potentially harmful for mothers and neonates, this may be an important factor in the increased risk of diseases occurring during this period.

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“…A ROS accumulation results in oxidative damage to lipids, proteins and DNA and consequently tissue injury, therefore the oxidative stress is involved in pathogenesis of many diseases. In pigs, oxidative stress has been linked to several diseases and clinical conditions such as experimental endotoxemia or septic shock [3], respiratory infections [4], porcine reproductive and respiratory syndrome (PRSS) [5], and skin disease [6]. ROS accumulation can also induce damage in the intestinal tissue, which can facilitate bacterial translocation and compromise the intestinal barrier integrity in pigs [2].…”

Section: Introductionmentioning

confidence: 99%

Biomarkers of oxidative stress in saliva in pigs: analytical validation and changes in lactation

et al. 2019

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Background Biomarkers of oxidative stress in pigs have been measured in serum/plasma samples. However, blood collection in pigs can be highly stressful to the animals. Saliva is a biological fluid with several advantages in pigs over blood, since it can be easily collected without stress to the animals, being therefore an ideal sample in this species. The objective of this study was the validation of assays for the evaluation of oxidative stress status in saliva of pigs. For this purpose, three assays commonly used to evaluate the total antioxidant capacity (TAC): trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP)), one individual antioxidant (uric acid) and two assays to evaluate oxidant concentrations (advanced oxidation protein products (AOPP) and hydrogen peroxide (H 2 O 2 )) were measured and validated in porcine saliva. In addition, the possible changes of these assays in sows’ saliva during lactation were be studied. Results The methods had intra- and inter-assays coefficient of variation lower than 15%. They also showed an adequate linearity and recovery, and their detection limits were low enough to detect the analytes in saliva of pigs. Overall the analytical validation tests showed that the assays used in our study are valid and reliable for the evaluation of oxidative stress in porcine saliva. In addition, it was observed that these salivary biomarkers can change in a situation of oxidative stress such as lactation in sows. Conclusions All assays for salivary biomarkers of oxidative stress evaluated in this study have demonstrated a high analytical accuracy and low imprecision. In addition, it has been observed that these biomarkers showed significant changes in a situation of oxidative stress such as lactation in sows. Therefore, this study opens a new possibility of using saliva as a non-invasive sample to evaluate oxidative stress in pigs.

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Assay of alterations in oxidative stress markers in pigs naturally infested with Sarcoptes scabiei var. suis

Cited by 16 publications

References 20 publications

Sarkoptik Uyuzlu Koyunlarda Serum Tiyol Disülfit Seviyeleri

Sarkoptik Uyuzlu Koyunlarda Serum Tiyol Disülfit Seviyeleri

Polish Journal of Veterinary Sciences

Biomarkers of oxidative stress in saliva in pigs: analytical validation and changes in lactation

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