Assay of ertapenem in human serum by high-performance liquid chromatography

Simultaneous determination of three carbapenem antibiotics in plasma by HPLC with ultraviolet detection

“…Lower LOQs (from 0.05 to 0.25 mg/L) were previously reported with methods using ultra-filtration or column switching [4,19,22,26,28]. Other published methods using protein precipitation showed LOQs similar to our method Table 1 Intra-and inter-day precision (CV) and accuracy (bias) of imipenem, meropenem and ertapenem (0.25-1 mg/L), but required much higher volume of plasma (up to 500 L) [15,17,29]. LOQ was sufficient for clinical applications, as reported minimal concentrations were higher than 0.5 mg/L at recommended dosages [2,4,5,35,36].…”

“…Several analytical methods based on high-performance liquid chromatography (HPLC) using ultraviolet (UV) detection have been reported for the analysis of a carbapenem in plasma or other biological fluids [4,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. Few of them required a simple sample preparation such as protein precipitation [16,17,29], but most of them required material, which is not available in every laboratory like column switching [4,[21][22][23]25,27] or ultra-filtration [4,15,19,26,28].…”

“…Few of them required a simple sample preparation such as protein precipitation [16,17,29], but most of them required material, which is not available in every laboratory like column switching [4,[21][22][23]25,27] or ultra-filtration [4,15,19,26,28]. Only one method described a simultaneous determination of several carbapenems in plasma but this method used capillary electrophoresis to separate the different compounds [32].…”

Pharmacodynamics of the Antibacterial Effect of and Emergence of Resistance to Doripenem in Pseudomonas aeruginosa and Acinetobacter baumannii in an In Vitro Pharmacokinetic Model

“…The injection volume was 5 l. A standard was included after every 6th sample. The intraassay precision was below 5% (16).…”

“…In addition, between 6 and 12 doses were simulated per strain in dose-ranging experiments designed to achieve a doripenem T MIC of 0 to 100% for each strain by changing the amount of drug given. In experiments to define the T MIC -antibacterial effect relationship in dose-fractionation experiments, T MIC targets of 12.5%, 25%, and 37.5% were achieved using six doses in 48 h, two doses in 48 h, and a single dose in 48 h. Human dose simulations of 500 mg, 1,000 mg, 2,000 mg, and 3,000 mg every 8 h were also performed over 96 h. Doripenem concentrations were determined using a modification of a validated in-house high-pressure liquid chromatography method for ertapenem (16). Chromatography was performed using a Concept II pump, a 310-nm detector, and a Hypersil 5 octyldecyl silane column (10 by 4.6 mm).…”