Assay of Muramidase Activity in Serum, Plasma, or Urine

Zucker, Stanley; Webb, Allen M.; Dubinsky, Eli

doi:10.1016/b978-0-12-609107-6.50009-x

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…No preservatives were used and, when the assays were not performed immediately, the specimens were stored at 4°C or -20°C. Both lysozyme and NAG are very stable enzymes in the urine (Burrows, 1967;Harrison et al, 1968; Shehadeh et al, 1970;Zucker and Webb, 1970; Sandman et al, 1973;Wellwood et al, 1973). Annals of Clinical Biochemistry, 1978, 15, 253-260 have been made in 35 patients after renal transplantation, and during 52 suspected rejection episodes.…”

Section: Urine Samplesmentioning

confidence: 99%

“…Moreover, both enzymes have in common relatively simple and easy methods of assay. A urinary lysozyme assay can be performed inside a matter of a few minutes by the method used in this paper (the cost is virtually negligible) and simple automated techniques have also been described (Burrows, 1967;Zucker and Webb, 1970), while NAG is measured by a rapid and sensitive fluorimetric technique (Leaback and Walker, 1961;.…”

Section: Patients With Acute Rejection Episodesmentioning

confidence: 99%

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Urinary Excretion of Lysozyme and N-Acetyl-β-D-Glucosaminidase in the Diagnosis of Renal Allograft Rejection

1978

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Garcia et al., 1975). The purpose of this study was to assess whether the measurements of both enzymes gave earlier prediction of graft rejection than either alone. URINE SAMPLESFor enzyme assay, freshly voided samples were collected and assayed, usually within 48 hours. No preservatives were used and, when the assays were not performed immediately, the specimens were stored at 4°C or -20°C. Both lysozyme and NAG are very stable enzymes in the urine (Burrows, 1967;Harrison et al., 1968; Shehadeh et al., 1970;Zucker and Webb, 1970; Sandman et al., 1973;Wellwood et al., 1973). Annals of Clinical Biochemistry, 1978, 15, 253-260 have been made in 35 patients after renal transplantation, and during 52 suspected rejection episodes. A close correlation has been found between these two parameters although they did not consistently change in parallel. The use of both assays may give a better indication of impending rejection than either assay alone.

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Section: Urine Samplesmentioning

confidence: 99%

Section: Patients With Acute Rejection Episodesmentioning

confidence: 99%