Assay of rotenone in river water by high‐throughput tandem mass spectrometry and multiple‐reaction monitoring methodology

Abstract

The high-throughput analytical approach proposed for the assay of rotenone in lake and river waters presented here is based on atmospheric pressure chemical ionization (APCI) multiple-reaction monitoring (MRM) methodology whereby the analyte and internal standard solutions are directly analyzed, without any previous chromatographic separation. The very good accuracy is obtained by the critical choice of a reference compound that shows gas-phase chemistry similar to that of the analyte, by producing the same product ions selected for the MRM-based assay.

Structural determination of the novel fragmentation routes of zwitteronic morphine opiate antagonists naloxonazine and naloxone hydrochlorides using electrospray ionization tandem mass spectrometry

“…A tentative breakdown pattern12, 15–17 of some of this major series of diagnostic product ions is presented in Scheme . In this validation, the product ion scan of the singly charged ion [M+H2HCl] + 1 afforded the ions 1a at m/z 633.25 and 1b at 615.24, by the respective elimination of either one or two molecules of water.…”

Using gamma distribution to determine half-life of rotenone, applied in freshwater

“…A standard curve of rotenone at known concentrations was used to calculate the concentration of rotenone in both water samples. This method is a modification of that published by Di Donna et al (2005). Modifications from the published procedure include: UV detection instead of mass spectrometry, a different UPLC gradient, different SPE cartridges for the sample concentration (Strata-X instead of C18) and a different sample volume.…”

“In-source” fragmentation of an isobaric impurity of lamotrigine for its measurement by liquid chromatography tandem mass spectrometry after pre-concentration using solid phase extraction

“…Although this method of fragmentation is less selective than tandem mass spectrometry, as all ions in the source will be fragmented simultaneously, it has been used by several research groups [4][5][6][7][8]. The most common method employed to achieve best specificity, selectivity and sensitivity in LC-MS/MS is MRM [9][10][11][12]. In this work, we combine the usage of in-source fragmentation with MRM to overcome the challenges posed by the fact that 14W80 is isobaric with lamotrigine.…”