Assaying Actual Hematein Content of Commercial Hematoxylins and Hemateins

Analytically pure hematoxylin (Htx), pentaacetylhematoxylin (PAHtx), and hematein (Hm) were isolated and characterized by 1H-NMR spectroscopy. The VIS/UV spectra of Htx and Hm were recorded in MeOH and in H2O at various pH values. The molar extinction coefficients of the long wavelength absorption bands are reported. The pKa value for the 1st acidic dissociation step of Hm has been determined from the pH dependency of the absorption spectra of Hm in aqueous buffer solutions. Finally, the absorption spectra are qualitatively discussed.

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New investigations on hematoxylin, hematein, and hematein-aluminium complexes

Bettinger

Zimmermann

1991

Histochemistry

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Standardization of biological dyes and stains: pitfalls and possibilities

Schulte

1991

Histochemistry

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The present paper gives a review of the actual state of standardization of biological dyes and stains. In a first part general information is given on practical problems encountered by the routine user of dyes with special emphasis on dye contamination. Some theoretical aspects of standardization are discussed. The second part of the paper gives more detailed information on commercial batches of hematoxylin-eosin-, Giemsa- and Papanicolaou-stains and on their standardization. Special problems arising with the application of image analysis techniques are briefly mentioned. User-oriented specifications for the standardization of dyes, stains and staining procedures are given. Fluorescent dyes and dyes used in chromogenic reagents such as the Feulgen-Schiff reaction are not included in this review.

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An electron spin resonance (ESR) study of free radicals formed from hematoxylin on tissue sections after blueing (alkalization)

Elek¹,

Rockenbauer

Jókay

1989

Histochemistry

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A free radical signal of 12 G width and g = 2.0045 can be observed in hematoxylin stained tissue blocks and sections. The amount of paramagnetic centres in stained specimens is significantly larger than in unstained ones. After alkalization simultaneously with the colour change the former free radical is detectable in hemalum precipitate and on stained paper strips. After solution of the stain in dioxane and alkalization, a well resolved hyperfine structure could be seen which could be assigned to three different radicals with the same g value as observed in the rigid matrix (tissue and paper). Quantitative evaluation of free radical concentration is also carried out for tissue sections.

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Assaying Actual Hematein Content of Commercial Hematoxylins and Hemateins

Cited by 10 publications

References 1 publication

New investigations on hematoxylin, hematein, and hematein-aluminium complexes

New investigations on hematoxylin, hematein, and hematein-aluminium complexes

Standardization of biological dyes and stains: pitfalls and possibilities

An electron spin resonance (ESR) study of free radicals formed from hematoxylin on tissue sections after blueing (alkalization)

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