Assaying for circulating osteopontin in practice: a technical note: Fig. 1—

Constantinescu, Daniela; Vornicu, M.; Grigoriu, Carmen; Cozmei, C.; Grigoriu, Bogdan

doi:10.1183/09031936.00160609

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…The reason of serum mean OPN differences between human studies were not clear. The results of circulating OPN levels may be variable depending on the type of sample (plasma vs serum), ages, races, detection method, and choice of ELISA kit (12, 15). …”

Section: Discussionmentioning

confidence: 99%

High Serum Osteopontin Levels Are Associated with Low Bone Mineral Density in Postmenopausal Women

Cho

Lee

et al. 2013

J Korean Med Sci

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Osteopontin (OPN) is an acidic, noncollagenous matrix protein produced by the bone and kidneys. It is reportedly involved in bone resorption and formation. We examined the association between serum OPN levels and bone mineral density in postmenopausal women. Premenopausal women (n=32) and postmenopausal women (n=409) participated in the study. We measured serum osteopontin levels and their relationships with bone mineral density and previous total fragility fractures. The postmenopausal women had higher mean serum OPN levels compared to the premenopausal women (43.6±25.9 vs 26.3±18.6 ng/mL; P<0.001). In the postmenopausal women, high serum OPN levels were negatively correlated with mean lumbar bone mineral density (BMD) (r=-0.113, P=0.023). In a stepwise multiple linear regression model, serum OPN levels were associated with BMD of the spine, femoral neck, and total hip after adjustment for age, body mass index, smoking, and physical activity in postmenopausal women. However, serum OPN levels did not differ between postmenopausal women with and without fractures. Postmenopausal women exhibit higher serum OPN levels than premenopausal women and higher serum OPN levels were associated with low BMD in postmenopausal women.

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Section: Discussionmentioning

confidence: 99%

High Serum Osteopontin Levels Are Associated with Low Bone Mineral Density in Postmenopausal Women

Cho

Lee

et al. 2013

J Korean Med Sci

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“…The levels of OPN in various cancers including ovarian, head and neck, breast, liver and cervical cancer are highly dependent on the ELISA kit utilized to measure its concentration. Significant differences in assay results with little correlation between those of the 2 commercial kits have been observed 52 . The lack of a strong correlation between the 2 methods indicates a systematic bias proportional to the measured value (proportional error).…”

Section: Discussionmentioning

confidence: 99%

“…The lack of a strong correlation between the 2 methods indicates a systematic bias proportional to the measured value (proportional error). The proportional error is due to the nonlinearity of the 2 measures and the amplification generated by different enzymes in the ELISA assay 52 . Since both assays measure the same target molecule, it is difficult to precisely determine which method is biased and in which direction 52 .…”

Section: Discussionmentioning

confidence: 99%

Plasma Osteopontin Level as a Diagnostic Marker of Hepatocellular Carcinoma in Patients with Radiological Evidence of Focal Hepatic Lesions

Khalil

Elgedawy

Faramawi

et al. 2013

Tumori

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“…Two different ELISA kits (IBL and R&D Systems) were used to determine FL-OPN levels because it has previously been demonstrated that these ELISA systems can have discordant results [35, 39, 40]. Analysis with the IBL kit showed that the levels of OPN were markedly elevated in both patients with DF (median, 25,951 pmol/L; 9.2-fold increase) and patients with DHF (median, 27,550 pmol/L; 9.7-fold increase), as compared with the HCs (2,814 pmol/L; Figure 1A).…”

Section: Resultsmentioning

confidence: 99%

Elevated levels of full-length and thrombin-cleaved osteopontin during acute dengue virus infection are associated with coagulation abnormalities

Chagan-Yasutan

Lacuesta²,

Ndhlovu

et al. 2014

Thrombosis Research

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Introduction Dengue virus (DENV) is transmitted by the mosquito vector, and causes a wide range of symptoms that lead to dengue fever (DF) or life-threatening dengue hemorrhagic fever (DHF). The host and viral correlates that contribute to DF and DHF are complex and poorly understood, but appear to be linked to inflammation and impaired coagulation. Full-length osteopontin (FL-OPN), a glycoprotein, and its activated thrombin-cleaved product, trOPN, integrate multiple immunological signals through the induction of pro-inflammatory cytokines. Materials and Method To understand the role of OPN in DENV-infection, we assessed circulating levels of FL-OPN, trOPN, and several coagulation markers (D-dimer, thrombin-antithrombin complex [TAT], thrombomodulin [TM], and ferritin in blood obtained from 65 DENV infected patients in the critical and recovery phases of DF and DHF during a dengue virus epidemic in the Philippines in 2010. Results Levels of FL-OPN, trOPN, D-dimer, TAT, and TM were significantly elevated in the critical phase in both the DF and DHF groups, as compared with healthy controls. During the recovery phase, FL-OPN levels declined while trOPN levels increased dramatically in both the DF and DHF groups. FL-OPN levels were directly correlated with D-dimer and ferritin levels, while the generation of trOPN was associated with TAT levels, platelet counts, and viral RNA load. Conclusion Our study demonstrated the marked elevation of plasma levels of FL-OPN and thrombin-cleaved OPN product, trOPN, in DENV-infection for the first time. Further studies on the biological functions of these matricellular proteins in DENV-infection would clarify its pathogenesis.

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Assaying for circulating osteopontin in practice: a technical note: Fig. 1—

Cited by 6 publications

References 10 publications

High Serum Osteopontin Levels Are Associated with Low Bone Mineral Density in Postmenopausal Women

High Serum Osteopontin Levels Are Associated with Low Bone Mineral Density in Postmenopausal Women

Plasma Osteopontin Level as a Diagnostic Marker of Hepatocellular Carcinoma in Patients with Radiological Evidence of Focal Hepatic Lesions

Elevated levels of full-length and thrombin-cleaved osteopontin during acute dengue virus infection are associated with coagulation abnormalities

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