Assembly and architecture of precursor nodes during fission yeast cytokinesis

Laporte, Damien; Coffman, Valerie C.; Lee, I-Ju; Wu, Jian-Qiu

doi:10.1083/jcb.201008171

Cited by 176 publications

(391 citation statements)

References 80 publications

(183 reference statements)

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“…Mid1 is the critical protein necessary for the recruitment and early scaffolding of these components [Wu et al, 2003;Celton-Morizur et al, 2004;Motegi et al, 2004;Wu et al, 2006;Coffman et al, 2009;Almonacid et al, 2011;Laporte et al, 2011;Padmanabhan et al, 2011].…”

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

“…Other major proteins recruited to the cytokinetic nodes besides the IQGAP protein Rng2 and myosin II are the F-BAR protein Cdc15 and the formin Cdc12 [Wu et al, 2003;Wu et al, 2006;Coffman et al, 2009;Laporte et al, 2011]. Careful analysis of the localization of contractile ring components in a variety of mutants has led to a model in which Mid1 organizes two independent modules.…”

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

“…This event is triggered by the Plo1-dependent phosphorylation of Mid1 N-terminal fragment which can bind Rng2 directly in vitro [Almonacid et al, 2011]. Noteworthy, the phosphorylation by Plo1 of the same set of amino acids within Mid1 1-100 fragment thus controls at the same time Mid1 activation and Mid1 export from the nucleus, directly linking these two events.Other major proteins recruited to the cytokinetic nodes besides the IQGAP protein Rng2 and myosin II are the F-BAR protein Cdc15 and the formin Cdc12 [Wu et al, 2003;Wu et al, 2006;Coffman et al, 2009;Laporte et al, 2011]. Careful analysis of the localization of contractile ring components in a variety of mutants has led to a model in which Mid1 organizes two independent modules.…”

mentioning

confidence: 99%

“…The second module is represented by the F-BAR protein Cdc15. Both modules contribute to the recruitment of the formin Cdc12 [Laporte et al, 2011]. …”

mentioning

confidence: 99%

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Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

Cytoskeleton

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Cell division is a critical and irreversible step in the cell cycle. The strategies that cells follow to regulate the position of the division plane must take into account the global geometry of the cell as well as position of the genetic material to ensure its accurate segregation into daughter cells of a given cell shape and size. Along the years, research on Schizosaccharomyces pombe, a wellrecognized model organism for cell division studies has allowed a detailed molecular understanding of the spatial mechanisms regulating cytokinesis. Division plane position in this unicellular rod-shaped organism, which divides by the assembly and constriction of a medially placed actomyosin ring, largely depends on the anillinlike protein Mid1. Therefore, the major pathways controlling the position of the division plane converge on Mid1. In this review, we make an overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells. These studies have revealed new mechanisms generating spatial information based on nuclear shuttling of the division plane factor Mid1 and on the establishment of cortical inhibitory gradients of the cell polarity kinase Pom1. V C 2012 Wiley Periodicals, Inc Key Words: cytokinesis, fission yeast, contractile ring, Mid1, cell division Introduction C ytokinesis is the final step of cell division that physically separates the daughter cells at the end of the cell cycle. It is a universal process, essential for cell proliferation, for the survival of unicellular organisms or for the development of multicellular organisms, whose major features have been well conserved along evolution. As an irreversible event, cytokinesis is under tight spatial and temporal regulation. Defective cytokinesis can indeed alter cell geometry or size, perturb cellular organization within tissues or prevent the accurate transmission of the genetic material, producing aneuploid cells, which can affect the survival of unicellular species or favor cancer and tumor progression in animal species.Cytokinesis is first strictly coordinated with mitotic progression in order to successfully fulfill chromosome segregation. Checkpoint mechanisms monitoring the function of the mitotic apparatus can halt cells in metaphase, which prevents cytokinesis until actual chromosome segregation in anaphase.Second, spatial regulatory pathways define the position of the division plane according to the position of the nucleus at mitotic entry, or of the mitotic apparatus, perpendicular to the axis of chromosome segregation. This ensures the efficient segregation of the genetic material and allows to control the geometry of the daughter cells in response to morphogenetic programs. Cell division can indeed be symmetric or asymmetric to allow the generation of daughter cells of different sizes. Orientation of the division plane is also modulated to properly position the daughter cells inside embryos or tissues and to permit the asymm...

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Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

mentioning

confidence: 99%

“…The second module is represented by the F-BAR protein Cdc15. Both modules contribute to the recruitment of the formin Cdc12 [Laporte et al, 2011]. …”

mentioning

confidence: 99%

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Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

Cytoskeleton

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“…The aforementioned nodes that contain myosin and formin nucleate actin filaments that are captured by neighboring nodes and pulled together (Vavylonis et al 2008). The barbed end of the growing actin filament is anchored by the nucleating formin at the donor node, and the pointed end of the filament is captured by myosin at the acceptor node (Laporte et al 2011). This interaction results in node congression at 30 nm/sec for a duration of 20 sec.…”

Section: Contractile Ring Assembly In Fission Yeastmentioning

confidence: 99%

Cytokinesis in Metazoa and Fungi

Glotzer

2016

Cold Spring Harb Perspect Biol

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SUMMARYCell division-cytokinesis-involves large-scale rearrangements of the entire cell. Primarily driven by cytoskeletal proteins, cytokinesis also depends on topological rearrangements of the plasma membrane, which are coordinated with nuclear division in both space and time. Despite the fundamental nature of the process, different types of eukaryotic cells show variations in both the structural mechanisms of cytokinesis and the regulatory controls. In animal cells and fungi, a contractile actomyosin-based structure plays a central, albeit flexible, role. Here, the underlying molecular mechanisms are summarized and integrated and common themes are highlighted.

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Regulation and Assembly of Actomyosin Contractile Rings in Cytokinesis and Cell Repair

Dekraker

Boucher

Mandato

2018

The Anatomical Record

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Cytokinesis and single‐cell wound repair both involve contractile assemblies of filamentous actin (F‐actin) and myosin II organized into characteristic ring‐like arrays. The assembly of these actomyosin contractile rings (CRs) is specified spatially and temporally by small Rho GTPases, which trigger local actin polymerization and myosin II contractility via a variety of downstream effectors. We now have a much clearer view of the Rho GTPase signaling cascade that leads to the formation of CRs, but some factors involved in CR positioning, assembly, and function remain poorly understood. Recent studies show that this regulation is multifactorial and goes beyond the long‐established Ca2+‐dependent processes. There is substantial evidence that the Ca2+‐independent changes in cell shape, tension, and plasma membrane composition that characterize cytokinesis and single‐cell wound repair also regulate CR formation. Elucidating the regulation and mechanistic properties of CRs is important to our understanding of basic cell biology and holds potential for therapeutic applications in human disease. In this review, we present a primer on the factors influencing and regulating CR positioning, assembly, and contraction as they occur in a variety of cytokinetic and single‐cell wound repair models. Anat Rec, 301:2051–2066, 2018. © 2018 Wiley Periodicals, Inc.

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Assembly and architecture of precursor nodes during fission yeast cytokinesis

Abstract: Mapping of fission yeast precursor node interaction modules and assembly reveals important steps in contractile ring assembly.

Cited by 176 publications

References 80 publications

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Cytokinesis in Metazoa and Fungi

Regulation and Assembly of Actomyosin Contractile Rings in Cytokinesis and Cell Repair

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