Assembly of Cas7 subunits of Leptospira on the mature crRNA of CRISPR-Cas I-B is modulated by divalent ions

Hussain, Md. Saddam; Kumar, Manish

doi:10.1016/j.gene.2022.146244

Cited by 4 publications

(2 citation statements)

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“…The overall goal is to elucidate the role of Cas proteins (Cas 6/7/8) in CRISPR adaptive immunity of the spirochetes and develop a CRISPR-Cas, an emerging microbial adaptive immune defense system of microbes (including spirochetes) against the foreign genetic elements that were being transferred horizontally from viruses or plasmid. Recent studies (from the lab of Manish Kumar, the 3 rd author) include transcriptional analysis of CRISPR 1-B array of L. interrogans, 60 assembly of Cas7 subunit of Leptospira, 61 and Cas2 protein of subtype I-C. 62…”

Section: Leptospirosis Research At Iit Guwahatimentioning

confidence: 99%

Epidemiological Status of Leptospirosis in India

Choudhary,

Kumar

et al. 2023

J. Pure Appl. Microbiol.

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A corkscrew-shaped spirochaete named Leptospira interrogans causes the infectious disease called leptospirosis. Leptospirosis, a growing public health concern worldwide, from subclinical infections to potentially lethal pulmonary hemorrhage. The disease caused by the pathogenic Leptospira, poses a threat to both humans and animals, and its transmission occurs through contact with infected animals, contaminated water, and soil. The prevalence of leptospirosis is influenced by various factors, including climate, urban development, and animal-rearing practices. It can manifest with severe symptoms in humans, making early diagnosis crucial. Diagnostic methods like microscopic agglutination test (MAT) and enzyme-linked immunoassay (ELISA) are widely used for the screening of leptospira infection. Molecular technique like PCR and qPCR offering higher sensitivity and rapidity. This paper meta-analyses the incidence of leptospirosis in various animals based on the literature published from 2005 to 2023 and provides prevalence of the disease in various animal including humans. Results suggest the significance prevalence of the disease in humans and various animal species, namely buffalo, rodents, and dogs. Coastal regions in India were particularly vulnerable to the disease. Efforts to control leptospirosis include surveillance programs and public health initiatives. Understanding the epidemiology and prevalence of leptospirosis, as highlighted in this paper, is essential for implementing effective preventive measures. Finally, a continued research, diagnostic advancements, public awareness campaigns and addressing research gaps in epidemiology of the disease are critical in mitigating the impact on human and animal health. This review provides important data for public health authorities, veterinarians, scientists and for the public, in general.

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Section: Leptospirosis Research At Iit Guwahatimentioning

confidence: 99%

Epidemiological Status of Leptospirosis in India

Choudhary,

Kumar

et al. 2023

J. Pure Appl. Microbiol.

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“…Among the Cas proteins (LinCas1-LinCas8) associated with the I-B system of sv. Copenhageni, LinCas1 (Dixit et al, 2021b), LinCas2 (Dixit et al, 2016), LinCas4 (Dixit et al, 2021a), LinCas6 (Prakash and Kumar, 2021), and LinCas7 (Hussain and Kumar, 2022) have been characterized to date. In this study, in the genome of L. interrogans sv.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of CRISPR I-B arrays of Leptospira interrogans serovar Lai and its processing by Cas6

Prakash

Kumar

2022

Front. Microbiol.

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In the genome of various Leptospira interrogans serovars, the subtype I-B locus of CRISPR-Cas possesses either one or multiple CRISPR arrays. In silico database (CRISPRCasdb) for predicting CRISPR-Cas reveals seven CRISPR arrays in L. interrogans serovar Lai positioned between the two independent cas-operons. Here, we present the redefined repeat-spacer boundaries of the CRISPR subtype I-B locus of serovar Lai. Such refinement of boundaries of arrays in serovar Lai was done after comparison with the characterized array of another serovar Copenhageni and the manual analysis of CRISPR flanking sequences. Using the reverse transcription-PCR (RT-PCR), we account that the seven CRISPR are transcriptionally active in serovar Lai. Our RT-PCR and quantitative real-time PCR analysis of transcripts in serovar Lai indicated that seven CRISPR of subtype I-B transcribe together as a single precursor unit. Moreover, the cleavage of the two miniature pre-crRNA of the subtype I-B by Cas6 demonstrates the biogenesis of the expected size of mature crRNA essential for the guided interference of foreign DNA. This study features insight into transcription direction and the crRNA biogenesis in serovar Lai essential for RNA-mediated interference of invading nucleic acids.

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