Assembly of synthetic peptide vaccines by chemoselective ligation of epitopes: influence of different chemical linkages and epitope orientations on biological activity

A Decaepitope Polypeptide Primes for Multiple CD8+ IFN-γ and Th Lymphocyte Responses: Evaluation of Multiepitope Polypeptides as a Mode for Vaccine Delivery

“…Further improvements in synthetic peptide chemistries may expand the size of polypeptides that are amenable to synthetic production. Potential alternatives include chemoselective ligation of purified intermediates (66). Alternatively, various recombinant expression systems could be used for generating large peptides such as transgenic plants (67), bacteria (40), yeast (68), and baculovirus-insect cells (28,69).…”

A Modular Approach to Assembly of Totally Synthetic Self-adjuvanting Lipopeptide-based Vaccines Allows Conformational Epitope Building

“…Three T H epitopes were used: (i) T MV , which has the sequence KLIPNASLIENCTKAEL and is derived from the fusion protein of the Morbillivirus canine distemper virus (16) and which demonstrates promiscuity between different MHC class II molecules from the same and different species; (ii) T FLU , which is derived from the light chain of influenza virus hemagglutinin, has the sequence ALNNRFQ-IKGVELKS (21),and is an effective T H epitope in BALB/c mice; and (iii) T OVA , which is derived from ovalbumin, has the sequence ISQAVHAAHAEINEAGR (22), and is an effective CD4 ϩ T H epitope in C57BL/6 mice. Peptide Synthesis-Peptides, lipopeptides, and lipopeptide modules were synthesized, purified, and characterized in house using Fmoc chemistry throughout as described elsewhere (5,11,13); the specific protocols used are detailed below.…”

Induction and detection of long-lasting peptide-specific antibody responses in pigs and beef cattle: a powerful technology for tracing meat processing chains from stock farmers to sales counters

“…In course of cross-linking reaction the peptide is bound to KLH via two stable bonds, a thioether and an amide bond. This prevents a rapid cleavage of peptide from KLH in vivo which would result in a loss of anti-peptide antibody response (Zeng, Ghosh, Macris, Pagnon, & Jackson, 2001). After a final dialysis step the conjugation solution contains: peptide coupled to KLH, NaCl, Na-phosphate, sucrose and Thiomersal to inhibit microbial growth.…”