Assembly of Water‐soluble AIE‐active Fluorescent Organic Nanoparticles for Ratiometric Detection of Hypochlorite in Living Cells

Abstract

Hypochlorous acid (HOCl) plays a crucial role in many physiological processes and is widely used as bleach, deodorant and fungicide. In this work, we designed an amphiphilic hydrazone fluorescent molecule THG‐1 containing hydrophilic sugar units and hydrophobic tetraphenylethylene unit for ratiometric detection of HOCl with high sensitivity and excellent selectivity based on HOCl‐triggered hydrolyzation reaction and aggregation‐induced emission (AIE) effect. The detection mechanism was verified by liquid chromatograph mass spectrometry experiments and scanning electron microscope (SEM) tests. Contrast experiments revealed that the numbers of lactose unit and hydrazone linker were essential for assembly of THG‐1 and detection of HOCl. In addition, THG‐1 was successfully used for imaging of exogenous and endogenous HOCl in living cells.

Ruthenium(II) Complex‐based Highly Specific Luminescence Light‐up Probe for Detecting HOCl via C(sp2)‐H Chlorination

“…Compared to the several analytical methods, [18][19][20][21][22] luminescent detection possesses promising features because of excellent selectivity, sensitivity, simple operation, and in vitro and in vivo HOCl imaging. [13,16,17,[23][24][25][26] Consequently, many luminescent probes have been developed for specific detection and imaging of HOCl. [16][17][18][19][20][21][22][23][24][25][26][27][28] To date, most of the fluorescent HOCl probes were designed based on the strong oxidation property of HOCl, such as oxidation of chalcogenides, p-methoxyphenol, BÀ H bond, pyrrole ring, and deoximation reactions.…”

“…Thus, highly selective and sensitive detection of HOCl has received great importance in chemistry and biology. Compared to the several analytical methods, [18–22] luminescent detection possesses promising features because of excellent selectivity, sensitivity, simple operation, and in vitro and in vivo HOCl imaging [13,16,17,23–26] . Consequently, many luminescent probes have been developed for specific detection and imaging of HOCl [16–28] .…”

Tetraphenylethene Functionalized Polyhedral Oligomeric Silsesquioxane Fluorescent Probe for Rapid and Selective Trifluralin Sensing in Vegetables and Fruits

“…TPE derivatives have attracted much attention for their facile synthesis, easy chemical modification, high thermal stability, and high fluorescence quantum yields [15] . Liu et al [16] . designed a fluorescent molecule THG‐1 containing sugar units and TPE units for imaging of hypochlorous acid in living cells.…”

Hydrazone-Based Amphiphilic Brush Polymer for Fast Endocytosis and ROS-Active Drug Release

“…These spectral changes suggest the dissociation of hydrazone and the concomitant formation of aldehyde, a reaction that has been previously reported. 35,44 The disassembly of the micelle was monitored by measuring the release of the cargo encapsulated within the micelle. Compound 8 (structure shown in Supporting Information) was selected as cargo due to its relative inertness against ClO − .…”