Assessing and Restoring Sperm Fertilizing Competence

Neri, Q.V.; Monahan, D.; Kocent, Justin; Hu, Jing-mei; Rosenwaks, Z.; Palermo, G.D.

doi:10.1016/j.fertnstert.2010.07.589

Cited by 6 publications

(7 citation statements)

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“…The identification of specific localization patterns of PLCζ in fertile males, and the precise functional relevance of PLCζ subcellular distribution, would provide a key benchmark to which suboptimal spermatozoa may be compared and can be used as a diagnostic tool . Previous work using immunofluorescent techniques has indicated that there appears to be a specific distribution of PLCζ in the sperm head, possibly in the perinuclear theca, that is consistent among fertile spermatozoa [29, 117], and an abnormal localization pattern is evident in spermatozoa that consistently failed to fertilize with ICSI [101, 117-119]. This argues for a correlation between an abnormal PLCζ activity within the sperm head and its ability to activate the oocyte at fertilization.…”

Section: How To Diagnose Fertilization Failurementioning

confidence: 94%

“…However, this is time consuming due to the need to stimulate and harvest mouse oocytes and be impractical or unachievable in a hospital or private setting. As we have tested in our practice, an alternative method would be to search for the presence of the PLCζ within the spermatozoa utilizing a polyclonal antibody [29, 119]. Cases of failed fertilization where lack of the sperm cytosolic factor have been clearly identified can be offered assisted oocyte activation.…”

Section: How To Diagnose Fertilization Failurementioning

confidence: 99%

“…Spermatozoa were exposed to SLO (30 min) prior to ICSI to assist in sperm membrane permeabilization [169]. Following sperm injection, oocytes were sequentially treated with Ca 2+ ionophore [119, 136, 155]. …”

Section: Treatment Optionsmentioning

confidence: 99%

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Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Neri¹,

Lee²,

Rosenwaks³

et al. 2014

Cell Calcium

131

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Summary Since the establishment of in vitro fertilization, it became evident that almost half of the couples failed to achieve fertilization and this phenomenon was attributed to a male gamete dysfunction. The adoption of assisted fertilization techniques particularly ICSI has been able to alleviate male factor infertility by granting the consistent ability of a viable spermatozoon to activate an oocyte. Single sperm injection, by pinpointing the beginning of fertilization, has been an invaluable tool in clarifying the different aspects of early fertilization and syngamy. However, even with ICSI some couples fail to fertilize due to ooplasmic dysmaturity in relation to the achieved nuclear maturation marked by the extrusion of the first polar body. More uncommon are cases where the spermatozoa partially or completely lack the specific oocyte activating factor. In this work, we review the most relevant aspects of fertilization and its failure through assisted reproductive technologies. Attempts at diagnosing and treating clinical fertilization failure are described.

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Section: How To Diagnose Fertilization Failurementioning

confidence: 94%

Section: How To Diagnose Fertilization Failurementioning

confidence: 99%

See 1 more Smart Citation

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Neri¹,

Lee²,

Rosenwaks³

et al. 2014

Cell Calcium

131

View full text Add to dashboard Cite

show abstract

“…Most aneuploid pregnancies do not survive, with the majority of losses occurring during the first few weeks of uterine life. In general, autosomal trisomies constitute the large majority of aneuploid embryos, with 16, 18 and 21 having a maternal origin and sex chromosomal aneuploidies (45X, 47XXY, 47XYY) often originating from the paternal origin ( Hassold & Hunt 2001 , Neri et al . 2014 a ).…”

Section: Male Gamete Bioassaysmentioning

confidence: 99%

“…2014 a ). While meiotic errors that lead to fetal aneuploidy can originate from either the male or female gamete, the occurrence is lower in spermatozoa (9%) when compared to oocytes (20%) ( Hassold & Hunt 2001 , Neri et al . 2014 a ).…”

Section: Male Gamete Bioassaysmentioning

confidence: 99%

Intracytoplasmic sperm injection: state of the art in humans

et al. 2017

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Among infertile couples, 25% involve both male and female factors, while male factor alone accounts for another 25% due to oligo-, astheno-, teratozoospermia, a combination of the three, or even a complete absence of sperm cells in the ejaculate and can lead to a poor prognosis even with the help of assisted reproductive technology (ART). Intracytoplasmic sperm injection (ICSI) has been with us now for a quarter of a century and in spite of the controversy generated since its inception, it remains in the forefront of the techniques utilized in ART. The development of ICSI in 1992 has drastically decreased the impact of male factor, resulting in millions of pregnancies worldwide for couples who, without ICSI, would have had little chance of having their own biological child. This review focuses on the state of the art of ICSI regarding utility of bioassays that evaluate male factor infertility beyond the standard semen analysis and describes the current application and advances in regard to ICSI, particularly the genetic and epigenetic characteristics of spermatozoa and their impact on reproductive outcome.

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Understanding the Spermatozoon

Neri

Rosenwaks

et al. 2014

Methods in Molecular Biology

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The former perception of the spermatozoon as a delivery device of the male genome has been expanded to include a new understanding of the cell's complex role in fertilization. Once the spermatozoon reaches the oocyte, it triggers egg activation and orchestrates the stages of pre- and post-fertilization in a preprogrammed pattern while tapping the oocyte's resources in an effort to generate a new life.

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Assessing and Restoring Sperm Fertilizing Competence

Cited by 6 publications

References 0 publications

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Intracytoplasmic sperm injection: state of the art in humans

Understanding the Spermatozoon

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