Assessing Biological Impacts of Land Reclamation in a Mining Region in Canada: Effects of Dolomitic Lime Applications on Forest Ecosystems and Microbial Phospholipid Fatty Acid Signatures

Nkongolo, K. K.; Michael, P.; Theriault, Gabriel; Narendrula, R.; Castilloux, P.; Kalubi, K. N.; Beckett, Peter; Spiers, Graeme

doi:10.1007/s11270-016-2803-5

Cited by 21 publications

(8 citation statements)

References 35 publications

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“…These results are consistent with data reported in other studies [4,6,14,16]. Likewise, strong positive correlations between soil pH and CEC reflect the data documented in other soil analysis under different conditions [15,35,36]. PLFA and pyrosequencing data demonstrate that microbial biomass and relative abundance were significantly higher in limed sites compared to the adjacent unlimed areas.…”

Section: Discussionsupporting

confidence: 92%

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Microbial Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and Phospholipid Fatty Acid Analyses

Narendrula-Kotha

Nkongolo

2017

PLoS ONE

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AimsTo assess the effects of dolomitic limestone applications on soil microbial communities’ dynamics and bacterial and fungal biomass, relative abundance, and diversity in metal reclaimed regions.Methods and ResultsThe study was conducted in reclaimed mining sites and metal uncontaminated areas. The limestone applications were performed over 35 years ago. Total microbial biomass was determined by Phospholipid fatty acids. Bacterial and fungal relative abundance and diversity were assessed using 454 pyrosequencing. There was a significant increase of total microbial biomass in limed sites (342 ng/g) compared to unlimed areas (149 ng/g). Chao1 estimates followed the same trend. But the total number of OTUs (Operational Taxonomic Units) in limed (463 OTUs) and unlimed (473 OTUs) soil samples for bacteria were similar. For fungi, OTUs were 96 and 81 for limed and unlimed soil samples, respectively. Likewise, Simpson and Shannon diversity indices revealed no significant differences between limed and unlimed sites. Bacterial and fungal groups specific to either limed or unlimed sites were identified. Five major bacterial phyla including Actinobacteria, Acidobacteria, Chloroflexi, Firmicutes, and Proteobacteria were found. The latter was the most prevalent phylum in all the samples with a relative abundance of 50%. Bradyrhizobiaceae family with 12 genera including the nitrogen fixing Bradirhizobium genus was more abundant in limed sites compared to unlimed areas. For fungi, Ascomycota was the most predominant phylum in unlimed soils (46%) while Basidiomycota phylum represented 86% of all fungi in the limed areas.ConclusionDetailed analysis of the data revealed that although soil liming increases significantly the amount of microbial biomass, the level of species diversity remain statistically unchanged even though the microbial compositions of the damaged and restored sites are different.Significance and Impact of the studySoil liming still have a significant beneficial effects on soil microbial abundance and composition > 35 years after dolomitic limestone applications.

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Section: Discussionsupporting

confidence: 92%

“…Bacterial communities are directly influenced by soil pH as most bacterial taxa exhibit narrow growth tolerances [7,15,18,43]. Rousk et al .…”

Section: Discussionmentioning

confidence: 99%

Microbial Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and Phospholipid Fatty Acid Analyses

Narendrula-Kotha

Nkongolo

2017

PLoS ONE

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“…But the two sites selected in Sudbury (Kelly Lake and Wahnapitae wet lands) are the most metal-contaminated within the CGS. This has resulted in a significant decrease and a possible loss of some alleles that has lead ultimately to a lower level of polymorphic loci in D. cespitosa populations sampled in 1999 (Nkongolo et al, 2016). Changes in genetic variation at the population level caused by metal contamination in other species has been reported in many studies (van Straalen and Timmermans, 2002;Lopes et al, 2004).…”

Section: Discussionmentioning

confidence: 97%

“…Changes in pH and elevated metal concentrations induce stress and limit plant growth, leading to erosion and the leaching of nutrients in the soils of the area (Winterhalder, 1996;Spiers et al, 2012;Nkongolo et al, 2013). While both regions have undertaken restoration efforts which include liming, seeding and capping, total levels of metals on-site are still high (Percival et al, 2007;Adamowicz, 2014;Nkongolo et al, 2013;2016).…”

Section: Introductionmentioning

confidence: 99%

“…ISSR is a reproducible nuclear marker that can be used when the sequence of the species being tested is not known (Nagaoka and Ogihara, 1997;Nkongolo et al, 2005;2016), allowing one to fingerprint and differentiate closely related populations (Raina et al, 2001;Nkongolo et al, 2003;. It can be used to assess genetic variability which can shift over generations.…”

Section: Introductionmentioning

confidence: 99%

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Reassessment of Molecular Variation in Isolated Populations of <i>Deschampsia cespitosa</i> from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic Recombination

Rainville¹,

Beckett²,

Nkongolo³

2017

American Journal of Environmental Sciences

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Abstract:The effects of ore extraction and processing procedures in the Greater Sudbury and Cobalt regions have been long-lasting. The objective of this study is to determine the current level of genetic variation in Deschampsia cespitosa populations from metal contaminated and uncontaminated sites in samples collected in Northern Ontario in 2016 after 17 years of potential genetic recombination since the last study in 1999. D. cespitosa leaf samples collected from the City of Greater Sudbury (CGS), Cobalt and Little Current were analyzed using ISSR primers. The levels of genetic variation were moderate to high within targeted populations. There was no significant difference (p≤0.05) in the overall percent of polymorphic loci in metal-uncontaminated site of Little Current (from 70% in 1999 to 77% in 2016) and in a Cobalt Cart Lake site (from 48% in 1999 to 55% in 2016). But a significant decrease in genetic variation was observed in CGS Wahnapitae site (from 72% in 1999 to 54% in 2016). On the other hand, a significant increase was observed in Cobalt Nipissing (from 46% in 1999 to 64% in 2016). The Kelly Lake site in the CGS with the lowest level of polymorphic loci (42.5%) in 2016 was not surveyed in 1999. The degree of genetic relatedness among sites has increased since the populations are more genetically closely related than 17 years ago. No population-specific ISSR marker was identified. The clustering of Cobalt and Sudbury populations strengthens the earlier theory that Sudbury populations of D. cespitosa might be from the Cobalt region.

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Microbial biomass and activity dynamics in restored lands in a metal contaminated region

et al. 2021

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Assessing Biological Impacts of Land Reclamation in a Mining Region in Canada: Effects of Dolomitic Lime Applications on Forest Ecosystems and Microbial Phospholipid Fatty Acid Signatures

Cited by 21 publications

References 35 publications

Microbial Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and Phospholipid Fatty Acid Analyses

Microbial Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and Phospholipid Fatty Acid Analyses

Reassessment of Molecular Variation in Isolated Populations of <i>Deschampsia cespitosa</i> from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic Recombination

Microbial biomass and activity dynamics in restored lands in a metal contaminated region

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