Microbial biomass and activity dynamics in restored lands in a metal contaminated region

McKergow, M.; Narendrula-Kotha, Ramya; Beckett, Peter; Nkongolo, K. K.

doi:10.1007/s10646-021-02464-9

Cited by 6 publications

(7 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase of CBH activity in soils with high organic N content has been reported in Saiya-Cork et al (2002). The high level of CBH activities in revegetated sites compared to untreated areas in the present study is consistent with previous reports that showed an elevated levels of CBH in limed or fertilized lands compared to untreated areas with lower organic matters (McKergow et al, 2021;Narendrula-Kotha R. and Nkongolo K. K., 2017, 2017b, 2017c. 4.4.3 Activity of β-N-acetylglucosaminidase β-N-acetylglucosaminidase is involved in the cycling of carbon and nitrogen and the degradation of chitin by hydrolyzing β-1,4glycosidic bonds of chitooligosaccharides into N-acetylglucosamine (Verchot and Borelli, 2005;Sinsabaugh et al, 2008).…”

Section: Activity Of Cellobiohydrolasesupporting

confidence: 93%

“…Narendrula-Kotha and Nkongolo (2017c) reported a significantly higher activities levels of BG, CBH, NAGase, AS, AP, AlP, GAP, and LAP in non-tailing sites limed >35 years ago compared to adjacent unlimed area in the CGS. McKergow et al (2021) on the other end showed an increase of BG, CBH and AS but a decrease of GAP activities in recently limed areas compared to untreated areas. They observed no significant change in AlP, AP, LAP, and NAGase activities when limed and unlimed soils were compared.…”

Section: Enzymatic Activitiesmentioning

confidence: 90%

“…It has been also found higher in reclaimed areas with higher level of organic matters. Previous studies revealed significantly higher level of NAGase activity in limed and reference soils contaminated with metals compared to metal contaminated unlimed sites (McKergow et al, 2021;Narendrula-Kotha R. and Nkongolo K. K., 2017, 2017c, 2017b.…”

Section: Activity Of Cellobiohydrolasementioning

confidence: 94%

“…There is a strong evidence that phosphatase enzymes are significantly correlated with all functional genes involved in the phosphorous nutrient cycling (Xue et al, 2018). Phosphatases can help increase the bioavailable amount of mineralized phosphorous (Acosta-Martinez and Tabatabai, 2000;McKergow et al, 2021). They are also used to catalyze the hydrolysis of both esters and anhydrides of phosphoric acid.…”

Section: Activity Of Acid and Alkaline Phosphatasementioning

confidence: 99%

“…Leucine aminopeptidase (LAP) hydrolyzes leucine and other hydrophobic amino acids from N terminus of polypeptides (Sinsabaugh et al, 2008). LAP and GAP activities are strongly associated with soil pH (Sinsabaugh et al, 2008;Narendrula-Kotha and Nkongolo, 2017c;McKergow et al, 2021).…”

Section: Activity Of Aminopeptidasementioning

confidence: 99%

See 4 more Smart Citations

Effects of Phytoremediation on Microbial Biomass, Composition, and Function in a Sulphide-Rich Tailing From a Metal-Contaminated Region

Nkongolo

Spiers²,

Beckett³

et al. 2022

Front. Environ. Sci.

Self Cite

View full text Add to dashboard Cite

Mining activities lead to serious land deterioration and large scale mine waste generation. Reclamation has been carried out on several technogenic materials to encourage the development of soils. To date no detailed studies have been conducted to assess if soil developed in reclaimed tailings can be suitable for microbial community sustainability and associated plant population. This study investigated if 1) soil metal contamination affects microbial biomass and composition in sulphide tailings and 2) phytoremediation of tailing increases microbial abundance, diversity, and function. Microbial biomass was assessed using Phospholipid fatty acid analysis (PLFA). Soil bacterial and fungal microbiota was determined by high throughput sequencing of 16S rRNA gene for bacteria and internal transcribed spacer region for fungi using the Illumina platform. Total copper, nickel, iron, and titium were higher in unreclaimed sites compared to vegetated areas but the total microbial biomass was significantly higher in reclaimed sites compared to reference areas. More importantly, the levels of microbial biomass were not impacted by metals since the bioavailable Cu, Ni, and Ti were low in all the sites. Site-specific bacterial and fungal genera were identified. Proteobacteria was the most dominant bacterial phylum while Ascomicota was the predominant fungal phylum. Interestinlgy, Acidiferrobacter, an acidophilic, thermotolerant and facultatively anaerobic was the most predominant genus in unreclaimed site that is characterized by extreme acidity (pH = 2.8). Analysis of microbial diversity revealed higher Chao 1, # of OTUs, Shannon index, and species richness in bacterial and fungal populations from reclaimed sites compared to controls. The levels of β-glucosidase (BG), cellobiohydrolase (CBH), β-N-acetylglucosaminidase (NAGase), aryl sulfatase (AS), acid phosphatase (AP), alkaline phosphatase (AlP), glycine aminopeptidase (GAP), and leucine aminopeptidase (LAP) activities were significantly higher in vegetated sites compared to reference areas. Strong positive correlation coefficients were observed between soil organic matter and total microbial biomass (r = 0.99). These two factors were positively correlated with enzymatic activities and bacterial population diversity. Overall, newly developed soils can sustain diverse microbial communities and associated vegetations.

show abstract