Assessing efficacy of N-Acetyl-l-Cysteine–Sodium Hydroxide on bacterial viability and enhanced recovery of Mycobacterium avium subsp. paratuberculosis from bovine colostrum

Hassan, Abdulwahed Ahmed; Khan, Izhar U. H.; Ganz, Sebastian; Wehrend, Axel; Failing, Klaus; Eisenberg, Tobias; Abdulmawjood, Amir; Bülte, Michael

doi:10.1016/j.mimet.2020.105968

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…Samples (such as sputum) that are contaminated with normal flora must undergo decontamination before culture. The laboratory can use a routine decontamination reagent such as NaOH together with N-acetyl-L-cysteine (NALC), which kills rapidly growing bacteria and fungi but has a limited effect on Mtb growth [ 19 ]. The laboratory should determine the optimal concentration of decontamination reagent, to avoid over-decontamination (which reduces the yield of Mtb) and under-decontamination (which leads to failed cultures, because of high rates of bacterial or fungal growth).…”

Section: Mtb Diagnosismentioning

confidence: 99%

Review and Updates on the Diagnosis of Tuberculosis

Huang

Lin

Wang

et al. 2022

JCM

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Diagnosis of tuberculosis, and especially the diagnosis of extrapulmonary tuberculosis, still faces challenges in clinical practice. There are several reasons for this. Methods based on the detection of Mycobacterium tuberculosis (Mtb) are insufficiently sensitive, methods based on the detection of Mtb-specific immune responses cannot always differentiate active disease from latent infection, and some of the serological markers of infection with Mtb are insufficiently specific to differentiate tuberculosis from other inflammatory diseases. New tools based on technologies such as flow cytometry, mass spectrometry, high-throughput sequencing, and artificial intelligence have the potential to solve this dilemma. The aim of this review was to provide an updated overview of current efforts to optimize classical diagnostic methods, as well as new molecular and other methodologies, for accurate diagnosis of patients with Mtb infection.

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Section: Mtb Diagnosismentioning

confidence: 99%

Review and Updates on the Diagnosis of Tuberculosis

Huang

Lin

Wang

et al. 2022

JCM

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“…As JD is incurable, early detection and isolation of infected animals prior to shedding is critical to reduce disease incidence. Fecal PCR and serum ELISA have been widely used to detect infected cattle, but their sensitivity is low, as shedding occurs intermittently, and serum antibody titers against MAP only increase in late stages of infection [ 3 , 4 , 15 , 16 , 17 ]. Economic losses, animal welfare concerns, and potential zoonotic risks associated with JD have driven research to improve early diagnosis and new diagnostic techniques [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of Transcriptome Profiles and lncRNA–miRNA–mRNA Competing Endogenous RNA Regulatory Network to Identify Biological Functional Effects of Genes and Pathways Associated with Johne’s Disease in Dairy Cattle

Ghafouri,

Dehghanian Reyhan,

Sadeghi

et al. 2024

ncRNA

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Paratuberculosis or Johne’s disease (JD), a chronic granulomatous gastroenteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), causes huge economic losses and reduces animal welfare in dairy cattle herds worldwide. At present, molecular mechanisms and biological functions involved in immune responses to MAP infection of dairy cattle are not clearly understood. Our purpose was to integrate transcriptomic profiles and competing endogenous RNA (ceRNA) network analyses to identify key messenger RNAs (mRNAs) and regulatory RNAs involved in molecular regulation of peripheral blood mononuclear cells (PBMCs) for MAP infection in dairy cattle. In total, 28 lncRNAs, 42 miRNAs, and 370 mRNAs were identified by integrating gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. In this regard, we identified 21 hub genes (CCL20, CCL5, CD40, CSF2, CXCL8, EIF2AK2, FOS, IL10, IL17A, IL1A, IL1B, IRF1, MX2, NFKB1, NFKBIA, PTGS2, SOCS3, TLR4, TNF, TNFAIP3, and VCAM1) involved in MAP infection. Furthermore, eight candidate subnets with eight lncRNAs, 29 miRNAs, and 237 mRNAs were detected through clustering analyses, whereas GO enrichment analysis of identified RNAs revealed 510, 22, and 11 significantly enriched GO terms related to MAP infection in biological process, molecular function, and cellular component categories, respectively. The main metabolic-signaling pathways related to MAP infection that were enriched included the immune system process, defense response, response to cytokine, leukocyte migration, regulation of T cell activation, defense response to bacterium, NOD-like receptor, B cell receptor, TNF, NF-kappa B, IL-17, and T cell receptor signaling pathways. Contributions of transcriptome profiles from MAP-positive and MAP-negative sample groups plus a ceRNA regulatory network underlying phenotypic differences in the intensity of pathogenicity of JD provided novel insights into molecular mechanisms associated with immune system responses to MAP infection in dairy cattle.

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“…Calves are infected through contact with contaminated dairy products, soil, manure, water, etc. ( Park et al, 2017 ; Hassan et al, 2020 ). MAP can also be transmitted through mammary secretions, in utero , and via semen ( Abbas et al, 2011 ; Sorge et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of mRNA and circRNA Expression Profiles of Bovine Monocyte-Derived Macrophages Infected With Mycobacterium avium subsp. paratuberculosis

Yao

Wang

et al. 2022

Front. Microbiol.

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Mycobacterium avium subsp. paratuberculosis (MAP) is the pathogen of Johne’s disease (paratuberculosis), which mainly causes chronic infectious granulomatous enteritis in ruminants and has brought huge economic losses to animal husbandry. As a specific intracellular pathogen, when MAP invades the body, it is internalized by macrophages where it is able to replicate by inhibition of the phagosome maturation, escaping the host immune system and surviving, which leads to the spread of the disease. More recent studies have shown that circRNA is involved in many pathological and physiological processes of the body as the molecular sponge of miRNA, the scaffold of RNA binding protein and having the characteristic of being able to translate into protein. In this study, the mRNA and circRNA expression profiles of MAP-infected bovine monocyte-macrophages and uninfected bovine cells were analyzed by high-throughput sequencing. A total of 618 differentially expressed mRNA were screened out, including 322 upregulated mRNA and 296 downregulated mRNA. In addition, the analysis of circRNA differential expression profile showed 39 differentially expressed genes including 12 upregulated and 27 downregulated genes. Moreover, differential genes belonging to cytokine activity, chemokine activity, inflammatory reaction, apoptosis, and other functional groups related to macrophage immune response were significantly enriched in Gene Ontology (GO). Multiple signal pathways including NF-κB, MAPK, Toll-like receptor, IL-17, JAK-STAT, and other signaling pathways related to activating macrophage immune response were significantly enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, RT-qPCR technology verified the accuracy of the mRNA sequencing results. In this study, we have obtained the transcriptome information of mRNA and circRNA of bovine monocyte-macrophage infected with MAP. These results will provide data support for the further study of mRNA–miRNA–circRNA network and immune escape mechanism of MAP and will enrich the knowledge of the molecular immune mechanisms of Johne’s disease as well.

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Assessing efficacy of N-Acetyl-l-Cysteine–Sodium Hydroxide on bacterial viability and enhanced recovery of Mycobacterium avium subsp. paratuberculosis from bovine colostrum

Cited by 5 publications

References 35 publications

Review and Updates on the Diagnosis of Tuberculosis

Review and Updates on the Diagnosis of Tuberculosis

Integrated Analysis of Transcriptome Profiles and lncRNA–miRNA–mRNA Competing Endogenous RNA Regulatory Network to Identify Biological Functional Effects of Genes and Pathways Associated with Johne’s Disease in Dairy Cattle

Analysis of mRNA and circRNA Expression Profiles of Bovine Monocyte-Derived Macrophages Infected With Mycobacterium avium subsp. paratuberculosis

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