Assessing genetic diversity in indigenous Veneto chicken breeds using AFLP markers

Abstract

Genetic variation in four indigenous chicken breeds from the Veneto region of Italy was assessed using amplified fragment length polymorphism (AFLP) markers. A total of 99 individuals were analysed using three AFLP primer combinations that produced 70 polymorphisms. Four indigenous Veneto chicken breeds (Ermellinata, Padovana, Pépoi and Robusta) and a reference broiler line were included in the analysis. Breed-specific markers were identified in each breed. The expected heterozygosity did not differ significantly among the indigenous Veneto chicken breeds and the broiler line. The coefficient of gene variation (Gst) value across loci indicated that almost half of the total variability was observed among breeds. Nei's standard genetic distance between pairs of breeds showed that the distance between the broiler line and the Pépoi breed was greater than the distances between the broiler line and the other three chicken breeds. Cluster analysis based on standard genetic distances between breeds indicated that the Padovana and Pépoi breeds were closely related. Factorial analysis based on a binary matrix of the AFLP data showed a clear distinction of all breeds.

Genetic Diversity and Phylogenetic Relationship Analysis between Red Jungle Fowl and Domestic Chicken using AFLP Markers

“…Mekchay et al (2014) presented the same in a study of Thai chicken breeds using SNP markers developed by amplicon sequencing of AFLP-PCR products. The genetic distances between all possible combinations of domestic chicken populations (0.057 to 0.068) were observed lower than reported by De Marchi et al (2005) between all possible combinations of four indigenous chicken breeds (0.164 to 0.259) using AFLP markers.…”

“…It has been widely applied for assessing genetic diversity and phylogenetic relationship analysis in poultry birds (De Marchi et al, 2005;Gao et al, 2007). The aim of this study was to investigate the genetic diversity and phylogenetic relationships among wild RJF and domestic chicken breeds using AFLP markers.…”

Selection of discriminant SNP markers for breed and geographic assignment of Italian sheep

“…Assignment tests based on multilocus genotypes were traditionally carried out by using microsatellites (Casellas et al, 2004;Orrù et al, 2006;Dalvit et al, 2008) or AFLP markers (De Marchi et al, 2006;Negrini et al, 2007). However, the development of high throughput SNP platforms for livestock genomes has opened new perspectives for breed traceability.…”

Conservation of Local Turkish and Italian Chicken Breeds: A Case Study

“…The conservation effectiveness of the Co.Va. project is being improved by using advanced molecular genetic techniques, such as microsatellite markers and mitochondrial DNA (mtDNA) (Cassandro et al, 2004;De Marchi et al, 2006;Zanetti, 2009;Zanetti et al, 2010). Similarly, genetic characterisation of local breeds and species through the use of molecular markers associated to powerful statistical approaches is urgently needed in Turkey.…”

“…In December, birds are weighed and a blood sample is collected from all the males for DNA analysis. Until 2005, genotyping of the individual animals for the marker-assisted conservation scheme was carried out using the amplified fragment length polymorphism (AFLP) technique (De Marchi et al, 2006). After 2005, microsatellites were applied in the Veneto conservation scheme because they are well dispersed in the genome and highly polymorphic (Twito et al, 2007;Zanetti, 2009).…”