Summary 1.A crucial question in wildlife management concerns the definition of ecologically meaningful management units. Management action needs to be co-ordinated at the appropriate spatial scale. There are few practical tools for delimiting pertinent management units for large mammals such as ungulates. 2. Previous work has favoured a molecular approach to determine genetically distinct units, but this may be too costly for routine management. In addition, recent studies have revealed fine-scale heterogeneity in population dynamics of free-ranging ungulates in relation to environmental variability. 3. We combined spatial analysis of environmental heterogeneity (vegetation cover, topography and climate), animal morphology (jaw length) and genetic structure (microsatellites) to define biologically meaningful population units for roe deer Capreolus capreolus in the Belluno province, north-eastern Italy. Jaw length is a sensitive measure of density-dependence and a good proxy for spatial and temporal variation in roe deer population growth. 4. Spatial analysis of environmental variables suggested that the study area should be divided into two, or possibly four, biogeographical regions in relation to variation in altitude and habitat type. There was significant spatial variability in jaw length across the province that clustered into two main regions (with shorter jaws in the north compared with the south), which matched the previously defined biogeographical regions. This spatial structuring was also supported by microsatellite analysis, which revealed two genetically distinct populations, one in the north and one in the south. 5. Synthesis and applications . A multiparameter approach, combining environmental information with data on indices of density-dependence such as jaw length, could be extremely useful for defining ecologically meaningful management units. Indeed, monitoring spatial and temporal variation in jaw length could provide deer managers with a simple way to index population structure and fluctuations in time and space.

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Genetic variation in four indigenous chicken breeds from the Veneto region of Italy was assessed using amplified fragment length polymorphism (AFLP) markers. A total of 99 individuals were analysed using three AFLP primer combinations that produced 70 polymorphisms. Four indigenous Veneto chicken breeds (Ermellinata, Padovana, Pépoi and Robusta) and a reference broiler line were included in the analysis. Breed-specific markers were identified in each breed. The expected heterozygosity did not differ significantly among the indigenous Veneto chicken breeds and the broiler line. The coefficient of gene variation (Gst) value across loci indicated that almost half of the total variability was observed among breeds. Nei's standard genetic distance between pairs of breeds showed that the distance between the broiler line and the Pépoi breed was greater than the distances between the broiler line and the other three chicken breeds. Cluster analysis based on standard genetic distances between breeds indicated that the Padovana and Pépoi breeds were closely related. Factorial analysis based on a binary matrix of the AFLP data showed a clear distinction of all breeds.

SummaryA conservation scheme for local poultry breeds from the Veneto region (Italy), the Co.Va. (Conservazione e Valorizzazione di Razze Avicole Locali Venete - Conservation and valorisation of local poultry genetic resources of Veneto region) project began in 2000 with finance provided by the Veneto Region. This project involved four organic farms located in different areas (mountain, hill and plain) in the region. The local chicken breeds included in the project were the Robusta Maculata, Robusta Lionata, Ermellinata di Rovigo, Pépoi and Padovana; the local duck breeds were the Germana Veneta and Mignon; the local turkey breeds were the Bronzato Comune and Ermellinato di Rovigo; and the local guinea fowl breed was the Camosciata. All breeds are dual-purpose breeds and are utilized for meat and egg production. The Department of Animal Science of the University of Padova planned a marker-assisted conservation scheme based on the maintenance and multiplication of these breeds within their production system (in situ conservation scheme). The objectives of the farmers involved in this project were to use these genotypes to generate typical regional products for local markets, to support the development of economically marginal areas through the revaluation of local breeds and to allow the preservation of animal biodiversity.

Genetic diversity in two ancient indigenous chicken breeds of the Veneto region was assessed using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 63 individuals were analysed using three selected AFLP primer combinations that produced 66 clear polymorphisms. The breeds analyzed were the Padovana and the Polverara (two ancient breeds) and a reference broiler line. The expected heterozygosity (Het) did not differ significantly among breeds. The variability at AFLP loci was largely maintained across breeds, as indicated by the coefficient of genetic differentiation (Gst) value. The lowest genetic distance is found between the Padovana and Polverara breeds suggesting that they could be genetically close