Assessing microbial water quality: Electroanalytical approaches to the detection of coliforms

Bigham, Teri; Dooley, James; Ternan, Nigel G.; Snelling, William J.; Castelán, M.C. Héctor; Davis, James A.

doi:10.1016/j.trac.2019.115670

Cited by 12 publications

(6 citation statements)

References 44 publications

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“…Furthermore, the obligate anaerobes and host-specific viruses proposed as alternative indicators for MST are often not amenable to laboratory culture [43]. A range of alternative detection methods continue to be developed and are the subject of several recent comprehensive reviews [97,[128][129][130][131], with real-time polymerase chain reaction (qPCR) and related molecular methods that infer the presence of fecal microbes from their genetic material experiencing particularly widespread adoption [43].…”

Section: Molecular Methods: Challenges and Advancesmentioning

confidence: 99%

Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality

Holcomb

Stewart

2020

Curr Envir Health Rpt

117

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Purpose of Review Fecal contamination of water is a major public health concern. This review summarizes recent developments and advancements in water quality indicators of fecal contamination. Recent Findings This review highlights a number of trends. First, fecal indicators continue to be a valuable tool to assess water quality and have expanded to include indicators able to detect sources of fecal contamination in water. Second, molecular methods, particularly PCR-based methods, have advanced considerably in their selected targets and rigor, but have added complexity that may prohibit adoption for routine monitoring activities at this time. Third, risk modeling is beginning to better connect indicators and human health risks, with the accuracy of assessments currently tied to the timing and conditions where risk is measured. Summary Research has advanced although challenges remain for the effective use of both traditional and alternative fecal indicators for risk characterization, source attribution and apportionment, and impact evaluation.

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Section: Molecular Methods: Challenges and Advancesmentioning

confidence: 99%

Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality

Holcomb

Stewart

2020

Curr Envir Health Rpt

117

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“…The growing and mandatory need for real-time on-site tracking of quality of water and the environment necessitates an alternative reliable and sensitive technique which is affordable and exerts less pressure on the environment [ 27 ]. The electrochemical monitoring technique has emerged that promising platform for handy, affordable substitute with high selectivity and a low limit of detection [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization and Detection of Lead in Water Using SPCE Modified with BiONPs/PANI

et al. 2021

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The need for constant assessment of river water qualities for both aquatic and other biological survival has emerged a top priority, due to increasing exposure to industrial pollutants. A disposable screen print carbon electrode was modified with a conductive polymer (PANI) and Zn and/or Cu oxides NPs, obtained through bioreduction in citrus peel extracts (lemon and orange), for ultra-sensitive detection of PB2+, in the Crocodile River water sample. The synthesized materials were characterized with Fourier-transform infra-red spectroscopy (FTIR), ultra-violet visible spectroscopy (UV-Vis), and scanning electron microscopy (SEM). The SPC-modified electrodes designated as SPCE/LPE/BiONPs/PANI and SPCE/OPE/BiONPs/PANI were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) and eventually deployed in the electrochemical detection of PB2+ in water using square wave voltammetry (SWV) technique. The electrochemical responses of the modified electrodes for both CV and EIS in 0.1 M HCl demonstrated enhanced performance relative to the bare SPCE. A detection and quantification limit of 0.494 ppb and 1.647 were obtained at SPCE/LPE/BiONPs/PANI, respectively, while a detection and quantification limit of 2.79 ppb and 8.91 ppb, respectively, were derived from SPCE/OPE/BiONPs/PANI. The relative standard deviations (RSD) for SPC electrode at a 6.04 µM PB2+ analyte concentration was 4.76% and 0.98% at SPCE/LPE/BiONPs/PANI and SPCE/LPE/BiONPs/PANI, respectively. The effect of copper, zinc, iron, cobalt, nickel, and magnesium on the stripping peaks of PB2+ at SPCE/OPE/BiONPs/PANI, showed no significant change except for cobalt, with about 17.67% peak current drop. The sensors were assessed for possible determination of PB2+ in spiked river water samples. The average percentage recovery and RSD calculated were 94.25% and 3.74% (n = 3) at SPCE/LPE/BiONPs/PANI and, 96.70% and 3.71% (n = 3) at SPCE/OPE/BiONPs/PANI, respectively. Therefore, the fabricated sensor material could be used for environmental assessment of this highly toxic heavy metal in the aquatic system

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“…[5] For the detection of E. coli , traditional plate counting method is reliable but labor-intensive and time-consuming. [6] In order to meet the growing demand for sensitive and rapid bacteria detection, various technologies have been developed, such as enzyme-linked immune sorbent assay, [7] polymerase chain reaction, [8] cell counting via flow cytometry, [9] quartz crystal microbalance, [10] nanosensor [11] and so on. While these detection methods are accurate and sensitive, each technique still has its own limitations.…”

Section: Introductionmentioning

confidence: 99%

Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T

Zhang

Mou

Ding

et al. 2021

Electrochimica Acta

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Assessing microbial water quality: Electroanalytical approaches to the detection of coliforms

Cited by 12 publications

References 44 publications

Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality

Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality

Electrochemical Characterization and Detection of Lead in Water Using SPCE Modified with BiONPs/PANI

Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T

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