Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T

Zhang, Han; Mou, Junsong; Ding, Jiawang; Qin, Wei

doi:10.1016/j.electacta.2021.138408

Cited by 10 publications

(5 citation statements)

References 43 publications

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“…It has been described that, under the conditions of environmental stress (the presence of oxygen), B. thetaiotaomicron can easily express four C10 protease genes, degrading the protein of the QP/Tw80 surfactant system and favoring the release of Qt [ 72 ]. Likewise, in stress conditions, E. coli is able to activate the production of proteases at the extracellular level for better protein assimilation [ 73 , 74 ]. This can explain the higher percentage of Qt release of 32.26% in the presence of E. coli , with a significant difference ( p < 0.05) compared to the control (without bacteria) ( Figure 8 F).…”

Section: Resultsmentioning

confidence: 99%

Nanoemulsions Based on Soluble Chenopodin/Alginate Complex for Colonic Delivery of Quercetin

Intiquilla,

Arazo,

Gamboa

et al. 2024

Antioxidants

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Inflammatory bowel disease (IBD) is an autoimmune disorder caused by uncontrolled immune activation and the subsequent destruction of the colon tissue. Quercetin (Qt) is a natural antioxidant and anti-inflammatory agent proposed as an alternative to mitigate IBD. However, its use is limited by its low oral bioavailability. This study aimed to develop nanoemulsions (NEs) based on a soluble chenopodin/alginate (QPA) complex and Tween 80 (T80), intended for the colonic release of Qt, activated by the pH (5.4) and bacteria present in the human colonic microbiota. NEs with different ratios of QPA/Tw80 (F1-F6) were prepared, where F4Qt (60/40) and F5Qt (70/30) showed sizes smaller than 260 nm, PDI < 0.27, and high encapsulation efficiency (>85%). The stability was evaluated under different conditions (time, temperature, pH, and NaCl). The DSC and FTIR analyses indicated hydrophobic and hydrogen bonding interactions between QPA and Qt. F4Qt and F5Qt showed the greater release of Qt in PBS1X and Krebs buffer at pH 5.4 (diseased condition), compared to the release at pH 7.4 (healthy condition) at 8 h of study. In the presence of E. coli and B. thetaiotaomicron, they triggered the more significant release of Qt (ƒ2 < 50) compared to the control (without bacteria). The NEs (without Qt) did not show cytotoxicity in HT-29 cells (cell viability > 80%) and increased the antioxidant capacity of encapsulated Qt. Therefore, these NEs are promising nanocarriers for the delivery of flavonoids to the colon to treat IBD.

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Section: Resultsmentioning

confidence: 99%

Nanoemulsions Based on Soluble Chenopodin/Alginate Complex for Colonic Delivery of Quercetin

Intiquilla,

Arazo,

Gamboa

et al. 2024

Antioxidants

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“…Alternatively, our group reported a magnetically controlled approach for biosensing using peptides as both recognition elements and signal reporters. [60,61] The extraction of hydrophilic bioreceptors can be dynamically controlled and modulated by a magnetic field into a polymeric membrane for potentiometric biosensing. The proposed generic platform can be used for potentiometric biosensing using other hydrophilic bioreceptors, such as peptides, aptamers and antibodies.…”

Section: Non-equilibrium Measurement Techniquesmentioning

confidence: 99%

“…Changes in the extracellular K + concentration associated with living cells upon stimulation were monitored in real time. Alternatively, our group reported a magnetically controlled approach for biosensing using peptides as both recognition elements and signal reporters [60,61] . The extraction of hydrophilic bioreceptors can be dynamically controlled and modulated by a magnetic field into a polymeric membrane for potentiometric biosensing.…”

Section: Non‐equilibrium Measurement Techniquesmentioning

confidence: 99%

Modern Potentiometric Biosensing Based on Non‐Equilibrium Measurement Techniques

Mou,

Ding,

Qin

2023

Chemistry A European J

Self Cite

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Modern potentiometric sensors based on polymeric membrane ion‐selective electrodes (ISEs) have achieved new breakthroughs in sensitivity, selectivity, and stability and extended applications in environmental surveillance, medical diagnostics, and industrial analysis. Moreover, nonclassical potentiometry shows promise for many applications and opens up new opportunities for potentiometric biosensing. Here, we aim to provide a concept to summarize advances over the past decade in the development of potentiometric biosensors with polymeric membrane ISEs. This concept articulates sensing mechanisms based on non‐equilibrium measurement techniques. In particular, we emphasize new trends in potentiometric biosensing based on attractive dynamic approaches. Representative examples are selected to illustrate key applications under zero‐current conditions and stimulus‐controlled modes. More importantly, fruitful information obtained from non‐equilibrium measurements with dynamic responses can be useful for artificial intelligence (AI). The combination of ISEs with advanced AI techniques for effective data processing is also discussed. We hope that the concept will illustrate the great possibilities offered by non‐equilibrium measurement techniques and AI in potentiometric biosensing and encourage further innovations in this exciting field.

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“…In addition, new technology for pathogen detection often needs to have three parts: identification, transduction or amplification, and output signal. Among them, HCR is based on the specific recognition and triggering characteristics of target nucleic acid and has both easy modification of signal molecule transduction (such as electrochemical reagents [20], nanoparticles [21], fluorescent dyes [22], biotin, etc.) and good amplification ability, so that it can easily combined with various signal output platforms (such as colorimetry, fluorescence, electrochemistry, Raman scattering, chemiluminescence, etc.…”

Section: Hcr In Foodborne Pathogen Detectionmentioning

confidence: 99%

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Zhao,

Guo,

et al. 2023

Foods

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Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy. This has led to the development of more sensitive and accurate methods for detecting pathogenic bacteria. Many signal amplification techniques have been used to improve the sensitivity of foodborne pathogen detection. Among them, hybridization chain reaction (HCR), an isothermal nucleic acid hybridization signal amplification technique, has received increasing attention due to its enzyme-free and isothermal characteristics, and pathogenic bacteria detection methods using HCR for signal amplification have experienced rapid development in the last five years. In this review, we first describe the development of detection technologies for food contaminants represented by pathogens and introduce the fundamental principles, classifications, and characteristics of HCR. Furthermore, we highlight the application of various biosensors based on HCR nucleic acid amplification technology in detecting foodborne pathogens. Lastly, we summarize and offer insights into the prospects of HCR technology and its application in pathogen detection.

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Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T

Cited by 10 publications

References 43 publications

Nanoemulsions Based on Soluble Chenopodin/Alginate Complex for Colonic Delivery of Quercetin

Nanoemulsions Based on Soluble Chenopodin/Alginate Complex for Colonic Delivery of Quercetin

Modern Potentiometric Biosensing Based on Non‐Equilibrium Measurement Techniques

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

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