Assessing photosystem I and II distribution in leaves from C<sub>4</sub> plants using confocal laser scanning microscopy

Pfündel, Erhard; Neubohn, B.

doi:10.1046/j.1365-3040.1999.00521.x

Cited by 28 publications

(19 citation statements)

References 22 publications

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“…6b). Both observations are consistent with the higher PS I/PS II concentration ratios in leaves of NADP-ME C 4 -type plants like maize than in barley leaves with C 3 photosynthesis (Edwards andWalker 1983, Pfündel andNeubohn 1999). Possibly, specific leaf optical properties associated to the Kranz anatomy of the maize leaf might further improve light absorption by PS I.…”

Section: Ps I and Ps Ii Spectrasupporting

confidence: 72%

Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of F V/F M on excitation wavelength

Pfündel

2009

Photosynth Res

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The F(0) and F(M) level fluorescence from a wild-type barley, a Chl b-less mutant barley, and a maize leaf was determined from 430 to 685 nm at 10 nm intervals using pulse amplitude-modulated (PAM) fluorimetry. Variable wavelengths of the pulsed excitation light were achieved by passing the broadband emission of a Xe flash lamp through a birefringent tunable optical filter. For the three leaf types, spectra of F(V)/F(M) (=(F(M) - F(0))/F (M)) have been derived: within each of the three spectra of F(V)/F(M), statistically meaningful variations were detected. Also, at distinct wavelength regions, the (V)/F(M) differed significantly between leaf types. From spectra of F(V)/F (M), excitation spectra of PS I and PS II fluorescence were calculated using a model that considers PS I fluorescence to be constant but variable PS II fluorescence. The photosystem spectra suggest that LHC II absorption results in high values of F(V)/F(M) between 470 and 490 nm in the two wild-type leaves but the absence of LHC II in the Chl b-less mutant barley leaf decreases the F(V)/F(M) at these wavelengths. All three leaves exhibited low values of F(V)/F(M) around 520 nm which was tentatively ascribed to light absorption by PS I-associated carotenoids. In the 550-650 nm region, the F(V)/F(M) in the maize leaf was lower than in the barley wild-type leaf which is explained with higher light absorption by PS I in maize, which is a NADP-ME C(4) species, than in barley, a C(3) species. Finally, low values of F(V)/F(M) at 685 in maize leaf and in the Chl b-less mutant barley leaf are in agreement with preferential PS I absorption at this wavelength. The potential use of spectra of the F(V)/F(M) ratio to derive information on spectral absorption properties of PS I and PS II is discussed.

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Section: Ps I and Ps Ii Spectrasupporting

confidence: 72%

Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of F V/F M on excitation wavelength

Pfündel

2009

Photosynth Res

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“…A reduced amount of PSII per PSI in BSCs would not only promote a higher cyclic electron transport, but will also reduce the amount of oxygen produced in the cell, thus further reducing the oxidative pathway of RuBisCO. Similarly, a lower amount of PSII per PSI was previously reported in studies using confocal microscopy on NADP-ME species (Pfündel and Neubohn 1999) and by immunoblots in maize (Darie et al 2006).…”

Section: Environmental Plasticity Of the Thylakoid Complexessupporting

confidence: 79%

Comparative analysis of thylakoid protein complexes in the mesophyll and bundle sheath cells from C₃, C₄ and C₃–C₄ Paniceae grasses

Hernández‐Prieto

Foster

Watson‐Lazowski

et al. 2019

Physiologia Plantarum

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To better understand the coordination between dark and light reactions during the transition from C3 to C4 photosynthesis, we optimized a method for separating thylakoids from mesophyll (MC) and bundle sheath cells (BSCs) across different plant species. We grew six Paniceae grasses including representatives from the C3, C3–C4 and C4 photosynthetic types and all three C4 biochemical subtypes [nicotinamide adenine dinucleotide phosphate‐dependent malic enzyme (NADP‐ME), nicotinamide adenine dinucleotide‐dependent malic enzyme (NAD‐ME) and phosphoenolpyruvate carboxykinase (PEPCK)] in addition to Zea mays under control conditions (1000 μmol quanta m−2 s−1 and 400 ppm of CO2). Proteomics analysis of thylakoids under native conditions, using blue native polyacrylamide gel electrophoresis followed by liquid chromatography‐mass spectrometry (LC‐MS), demonstrated the presence of subunits of all light‐reaction‐related complexes in all species and cell types. C4 NADP‐ME species showed a higher photosystems I/II ratio and a clear accumulation of the NADH dehydrogenase‐like complexes in BSCs, while Cytb6f was more abundant in BSCs of C4 NAD‐ME species. The C4 PEPCK species showed no clear differences between cell types. Our study presents, for the first time, a good separation between BSC and MC for a C3–C4 intermediate grass which did not show noticeable differences in the distribution of the thylakoid complexes. For the NADP‐ME species Panicum antidotale, growth at glacial CO2 (180 ppm of CO2) had no effect on the distribution of the light‐reaction complexes, while growth at low light (200 μmol quanta m−2 s−1) promoted the accumulation of light‐harvesting proteins in both cell types. These results add to our understanding of thylakoid distribution across photosynthetic types and subtypes, and introduce thylakoid distribution between the MC and BSC of a C3–C4 intermediate species.

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“…Hatch 1987;Pfundel and Neubohn 1999). There were high correlations between the granal index of the BS chloroplasts and the proportions of mitochondria and peroxisomes in the BS among C 4 species.…”

Section: Discussionmentioning

confidence: 88%

Structural and biochemical bases of photorespiration in C4 plants: quantification of organelles and glycine decarboxylase

Yoshimura

Kubota

Ueno

2004

Planta

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In C(4) plants, photorespiration is decreased relative to C(3) plants. However, it remains unclear how much photorespiratory capacity C(4) leaf tissues actually have. We thoroughly investigated the quantitative distribution of photorespiratory organelles and the immunogold localization of the P protein of glycine decarboxylase (GDC) in mesophyll (M) and bundle sheath (BS) cells of various C(4) grass species. Specific differences occurred in the proportions of mitochondria and peroxisomes in the BS cells (relative to the M cells) in photosynthetic tissues surrounding a vein: lower in the NADP-malic enzyme (NADP-ME) species having poorly formed grana in the BS chloroplasts, and higher in the NAD-malic enzyme (NAD-ME) and phosphoenolpyruvate carboxykinase (PCK) species having well developed grana. In all C(4) species, GDC was localized mainly in the BS mitochondria. When the total amounts of GDC in the BS mitochondria per unit leaf width were estimated from the immunogold labeling density and the quantity of mitochondria, the BSs of NADP-ME species contained less GDC than those of NAD-ME or PCK species. This trend was also verified by immunoblot analysis of leaf soluble protein. There was a high positive correlation between the degree of granal development (granal index) in the BS chloroplasts and the total amount of GDC in the BS mitochondria. The variations in the structural and biochemical features involved in photorespiration found among C(4) species might reflect differences in the O(2)/CO(2) partial pressure and in the potential photorespiratory capacity of the BS cells.

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Assessing photosystem I and II distribution in leaves from C₄ plants using confocal laser scanning microscopy

Cited by 28 publications

References 22 publications

Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of F V/F M on excitation wavelength

Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of F V/F M on excitation wavelength

Comparative analysis of thylakoid protein complexes in the mesophyll and bundle sheath cells from C₃, C₄ and C₃–C₄ Paniceae grasses

Structural and biochemical bases of photorespiration in C4 plants: quantification of organelles and glycine decarboxylase

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Assessing photosystem I and II distribution in leaves from C4 plants using confocal laser scanning microscopy

Cited by 28 publications

References 22 publications

Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of F V/F M on excitation wavelength

Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of F V/F M on excitation wavelength

Comparative analysis of thylakoid protein complexes in the mesophyll and bundle sheath cells from C3, C4 and C3–C4 Paniceae grasses

Structural and biochemical bases of photorespiration in C4 plants: quantification of organelles and glycine decarboxylase

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Assessing photosystem I and II distribution in leaves from C₄ plants using confocal laser scanning microscopy

Comparative analysis of thylakoid protein complexes in the mesophyll and bundle sheath cells from C₃, C₄ and C₃–C₄ Paniceae grasses