Assessing Retinal Neovascularization in an Animal Model of Proliferative Retinopathy

Penn, John S.; Henry, Mark M.

doi:10.1006/mvre.1996.0014

Cited by 10 publications

(5 citation statements)

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“…To determine the effect of the various treatments on angiogenesis, the extent of retinal neovascularization was assessed in flattened retinas stained for ADPase activity. 19 Images of ADPase-stained retinas were digitized, captured, and displayed at 65ϫ magnification. Vessel tufts were then outlined directly on the monitor face with an interactive stylus pen (FTG Data Systems, Stanton, CA).…”

Section: Retinal Angiogenesismentioning

confidence: 99%

Inhibition of Retinal Neovascularization by Intravitreal Injection of Human rPAI-1 in a Rat Model of Retinopathy of Prematurity

Penn¹,

Rajaratnam²

2003

Invest. Ophthalmol. Vis. Sci.

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Section: Retinal Angiogenesismentioning

confidence: 99%

Inhibition of Retinal Neovascularization by Intravitreal Injection of Human rPAI-1 in a Rat Model of Retinopathy of Prematurity

Penn¹,

Rajaratnam²

2003

Invest. Ophthalmol. Vis. Sci.

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“…1b). However, these sorts of images do not lend themselves readily to a quantitative analysis of NV, and are subject to several preparation artifacts (38). Therefore, an independent and more quantifiable additional analysis of the treatment groups was performed.…”

Section: Assessment Of Antineovascular Effectsmentioning

confidence: 99%

Adeno-associated virus type-2 expression of pigmented epithelium-derived factor or Kringles 1–3 of angiostatin reduce retinal neovascularization

Raisler

Berns

Grant

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

112

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Neovascular diseases of the retina include age-related macular degeneration and diabetic retinopathy, and together they comprise the leading causes of adult-onset blindness in developed countries. Current surgical, pharmaceutical, and laser therapies for age-related macular degeneration (AMD) rarely result in improved vision, do not significantly prevent neovascularization (NV), and often result in at least some vision loss. To address this therapeutic gap, we determined the efficacy of recombinant adeno-associated viral (rAAV) serotype-2-mediated expression of pigment epithelium-derived factor (PEDF) or Kringle domains 1-3 of angiostatin (K1K3) in reducing aberrant vessel formation in a mouse model of ischemia-induced retinal NV. Both PEDF and K1K3 are potent inhibitors of NV when injected directly, hence expression of these therapeutic factors from rAAV may provide long-term protection from neovascular eye disease. rAAV vectors expressing the therapeutic gene were injected into one eye of postnatal day 0 (P0) newborn mouse pups. Retinal NV was induced in P7 mice by exposure to elevated oxygen for 5 days followed by room air for another five days. Retinal NV was quantified by the number of vascular-endothelial-cell nuclei above the inner-limiting membrane in P17 eyes. The number of such vascular endothelial cell nuclei in eyes treated with rAAV-PEDF or rAAV-K1K3 was significantly reduced (both P < 0.0000002) compared with control eyes. Ocular protein levels detected by ELISA correlate well with the reduction in NV and confirm that expression of antineovascular agents from rAAV vectors may be a therapeutically useful treatment of retinal or choroidal neovascular disease.

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“…Therefore very similar results were obtained with single masked observers or using a median score for all three masked observers. Fluorescein perfusion and ADPase staining were performed on the same individual retinae as described by Penn and Henry (20) (Figs. 1 and 2).…”

Section: Resultsmentioning

confidence: 99%

The effect of carbon dioxide on oxygen-induced retinopathy in the neonatal rat

Holmes

Zhang

Lanier

1997

Current Eye Research

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Assessing Retinal Neovascularization in an Animal Model of Proliferative Retinopathy

Cited by 10 publications

References 0 publications

Inhibition of Retinal Neovascularization by Intravitreal Injection of Human rPAI-1 in a Rat Model of Retinopathy of Prematurity

Inhibition of Retinal Neovascularization by Intravitreal Injection of Human rPAI-1 in a Rat Model of Retinopathy of Prematurity

Adeno-associated virus type-2 expression of pigmented epithelium-derived factor or Kringles 1–3 of angiostatin reduce retinal neovascularization

The effect of carbon dioxide on oxygen-induced retinopathy in the neonatal rat

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