Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests

Pham, Mimi; Raymond, Jolia; Hester, Jonathan; Kyzar, Evan J.; Gaikwad, Siddharth; Bruce, Indya; Fryar, Caroline; Chanin, Simon; Enriquez, Joseph; Bagawandoss, Sidarth; Zapolsky, Ivan; Green, Jeremy; Stewart, Adam; Robison, Barrie D.; Kalueff, Allan V.

doi:10.1007/978-1-61779-597-8_17

Cited by 89 publications

(120 citation statements)

References 52 publications

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“…A total of 72 screenshots (24 per each shoal) per drug were used for analyses in this study, similar to Grossman et al (2010) and Pham et al (2012). Each screenshot was calibrated to the size of the tank and analyzed by trained observers, measuring the distances (cm) between each fish in the group using ImageTool software (University of Texas Health Sciences Center, San Antonio, TX), and averaging this data to obtain an average inter-fish distance per screenshot, as described in Grossman et al (2010).…”

Section: Behavioral Testing and Manual Analyses Of Shoaling Datamentioning

confidence: 99%

“…Simpler paradigms have included measuring the preference of a single zebrafish placed in a central compartment of a test tank flanked by two adjacent compartments which contain a shoal of conspecifics or are empty (Wright and Krause, 2006). More recent attempts to assess the internal dynamics of association among fish in free-swimming shoals have focused predominantly on measuring inter-and intra-fish distances over the course of a series of video frames (see Pham et al, 2012;Miller and Gerlai, 2007 for review).…”

Section: Introductionmentioning

confidence: 99%

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Automated high-throughput neurophenotyping of zebrafish social behavior

Green

Collins

Kyzar

et al. 2012

Journal of Neuroscience Methods

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159

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Section: Behavioral Testing and Manual Analyses Of Shoaling Datamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Automated high-throughput neurophenotyping of zebrafish social behavior

Green

Collins

Kyzar

et al. 2012

Journal of Neuroscience Methods

Self Cite

159

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“…The mirror-induced aggression (MIA) is a well-established and simple to record paradigm that can be used to quantify aggressive behavior [14][15][16][17] . Fish attacks their image reflected in a mirror because they consider it as if it was an intruder.…”

Section: Resultsmentioning

confidence: 99%

“…In this test, reduced latency to first contact with the mirror and increased mirror biting duration indicates increased aggressiveness [14,16] . Although it is observed an increase in the mirror biting duration for exposed groups at doses of 5, 40 and 60 mg.L -1 , this increase is not significant compared to the control group, according to analysis of variance.…”

Section: Resultsmentioning

confidence: 99%

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Investigation of ketamine pharmacological modulation of aggressive behavior in adult zebrafish

Campos

Martinis

2016

Neurosci Commun

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Ketamine is an anesthetic agent which in recent years has gained prominence due to its antidepressant properties. However, the adverse effects resulting from the acute administration of this drug have been reported and are a challenge for use of ketamine as an antidepressant. The aim of this study was the characterization of the acute effects of ketamine in states of aggressiveness in adult zebrafish (Danio rerio). Our results showed that ketamine didn't induce aggressive-like behaviors, at sub-anesthetic doses. However, these findings suggest that biochemical and neurochemical studies must be conducted to investigate the effects of ketamine on aggressiveness at molecular level in adult zebrafish.

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Knockout of tanc2 causes autism‐like behavior and sleep disturbance in zebrafish

et al. 2022

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Tanc2 is a large multi‐domain postsynaptic scaffold protein mainly expressed in the brain. In humans, tanc2 mutations have been associated with autism spectrum disorder (ASD) and other related neurodevelopmental disorders. However, the role of tanc2 in neurodevelopment and in controlling behaviors are not fully understood. Here, we generated and characterized a tanc2 knockout allele in zebrafish. Loss of tanc2 increases the larval brain size and body length by promoting proliferation and inhibiting apoptosis. We observed that the glutamatergic neuron population is significantly increased in tanc2 mutants while the GABAergic and the glycinergic neurons are not affected, suggesting that an excitatory/inhibitory (E/I) imbalance. Indeed, the tanc2 knockout larvae exhibited increase sleep. In adult zebrafish, the mutants display anxiolytic‐behavior, reduced aggression, and impaired social preference. The alterations in these behaviors are phenotypically similar to the ASD patients carrying tanc2 mutations. Therefore, the tanc2 knockout allele could serve as a valuable model to further study the role of tanc2 in the nervous system.

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Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests

Cited by 89 publications

References 52 publications

Automated high-throughput neurophenotyping of zebrafish social behavior

Automated high-throughput neurophenotyping of zebrafish social behavior

Investigation of ketamine pharmacological modulation of aggressive behavior in adult zebrafish

Knockout of tanc2 causes autism‐like behavior and sleep disturbance in zebrafish

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