Assessing the 3D Printability of an Elastomeric Poly(caprolactone-<i>co</i>-lactide) Copolymer as a Potential Material for 3D Printing Tracheal Scaffolds

V.G., Rahul; Wilson, Jijo; Thomas, Lynda V.; Nair, Prabha D.

doi:10.1021/acsomega.1c06679

Cited by 11 publications

(2 citation statements)

References 31 publications

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“…The final decomposition region after 500 °C could be attributed to the inorganic impurities [ 38 ]. To calculate the thermal stability of the hydrogels, we explained the degradation temperature as a temperature at which 50% of the sample degraded was taken [ 39 ]. This temperature for GM, GM/EMF, and GM/EMF/P hydrogels was 358.224, 350.79, and 371.182, respectively.…”

Section: Resultsmentioning

confidence: 99%

Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications

et al. 2023

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Gelatin methacrylate-based hydrogels (GelMA) were widely used in tissue engineering and regenerative medicine. However, to manipulate their various chemical and physical properties and create high-efficiency hydrogels, different materials have been used in their structure. Eggshell membrane (ESM) and propolis are two nature-derived materials that could be used to improve the various characteristics of hydrogels, especially structural and biological properties. Hence, the main purpose of this study is the development of a new type of GelMA hydrogel containing ESM and propolis, for use in regenerative medicine. In this regard, in this study, after synthesizing GelMA, the fragmented ESM fibers were added to it and the GM/EMF hydrogel was made using a photoinitiator and visible light irradiation. Finally, GM/EMF/P hydrogels were prepared by incubating GM/EMF hydrogels in the propolis solution for 24 h. After various structural, chemical, and biological characterizations, it was found that the hydrogels obtained in this study offer improved morphological, hydrophilic, thermal, mechanical, and biological properties. The developed GM/EMF/P hydrogel presented more porosity with smaller and interconnected pores compared to the other hydrogels. GM/EMF hydrogels due to possessing EMF showed compressive strength up to 25.95 ± 1.69 KPa, which is more than the compressive strength provided by GM hydrogels (24.550 ± 4.3 KPa). Also, GM/EMF/P hydrogel offered the best compressive strength (44.65 ± 3.48) due to the presence of both EMF and propolis. GM scaffold with a contact angle of about 65.41 ± 2.199 θ showed more hydrophobicity compared to GM/EMF (28.67 ± 1.58 θ), and GM/EMF/P (26.24 ± 0.73 θ) hydrogels. Also, the higher swelling percentage of GM/EMF/P hydrogels (343.197 ± 42.79) indicated the high capacity of this hydrogel to retain more water than other scaffolds. Regarding the biocompatibility of the fabricated structures, MTT assay results showed that GM/EMF/P hydrogel significantly (p-value < 0.05) supported cell viability. Based on the results, it seems that GM/EMF/P hydrogel could be a promising biomaterial candidate for use in various fields of regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications

et al. 2023

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“…D. Nair and colleagues synthesized four different ratios of poly(caprolactone)-propyl cross-linking ester and tested their potential ability to 3D print tissues such as the trachea. 363 Ratios with higher propylene glycol ester content have better printability. 3D printed trachea scaffolds have mechanical properties and printability that match the natural trachea.…”

Section: Application Of Dbe For Tissue Repairmentioning

confidence: 99%

Degradable biomedical elastomers: paving the future of tissue repair and regenerative medicine

Jia,

Huang,

Dong

et al. 2024

Chem. Soc. Rev.

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Engineering Shape to Overcome Contraction: The Role of Polymer–Collagen Hybrids in Advanced Dermal Substitutes

Leon‐Valdivieso,

Bethry,

Pinese

et al. 2024

J Biomedical Materials Res

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Collagen gels are the standard dermal equivalents par excellence, however the problem of rapid cell‐mediated contraction remains unresolved. Therefore, the development of hybrid constructs (HCs) based on collagen and polymeric scaffolds is proposed to address the mechanical instability that usually limits the formation of new, functional tissue. Equally important, these synthetic structures should be temporary (degradable) while ensuring that cells are well‐adapted to the new extracellular environment. In this study, we screened a library of scaffolds made of various polymers, including homopolymers of polycaprolactone (PCL) and poly D,L‐lactide (PLA50), their blends (PCL/PLA50), and copolymers (poly(D,L‐lactide‐co‐caprolactone), PCLLA50) to prepare HCs in a layer‐by‐layer fashion. The properties of polymers and copolymers along with their processability by electrospinning and 3D‐printing were evaluated. Then, we assessed the HCs resistance toward cell‐mediated contraction as well as the degradation of the polymeric scaffolds. Our results indicate that scaffolds with higher PLA50 content (e.g., PLA50 100%, PCL/PLA50 or PCLLA50, both at 50/50 caprolactone‐to‐D,L‐lactide molar ratio) presented more drawbacks in terms of handleability and processing, while those with greater PCL presence showed structural steadiness and ease to use. All the scaffolds integrated well with the collagen gel to form the corresponding HCs. With few exceptions, the HCs demonstrated good resistance to cell‐derived contraction over 3 weeks. Notably, HCs based on PCLLA50 90/10 (both versions, electrospun or 3D‐printed) performed best, showing only a 5%–17% area reduction compared to the 93% observed in collagen‐only gels. This copolymer displayed hydrolytic degradation depending on its shape, with up to 45% and 65% loss of molecular weight for the electrospun and 3D‐printed forms, respectively, correlating with their progressive change in mechanical features. HCs containing PCLLA50 90/10 also exhibited a better fibroblast distribution, enhanced myofibroblastic differentiation, and a three‐fold increase in cell proliferation (when the electrospun type was used) compared to collagen controls. These findings were instrumental in selecting a potential HC that might be used for future experiments in vivo.

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Assessing the 3D Printability of an Elastomeric Poly(caprolactone-co-lactide) Copolymer as a Potential Material for 3D Printing Tracheal Scaffolds

Cited by 11 publications

References 31 publications

Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications

Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications

Degradable biomedical elastomers: paving the future of tissue repair and regenerative medicine

Engineering Shape to Overcome Contraction: The Role of Polymer–Collagen Hybrids in Advanced Dermal Substitutes

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