Assessing the depletion of lincomycin in feathers from treated broiler chickens: a comparison with the concentration of its residues in edible tissues

Pokrant, Ekaterina; Maddaleno, Aldo; Lobos, Ramón; Trincado, Lina; Lapierre, Lisette; Martín, Betty San; Cornejo, Javiera

doi:10.1080/19440049.2019.1662952

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…Two days later, it was not detected above the LOQ of the method. Pokrant et al. (2019) reported that the depletion time for LIN residue concentrations in feathers was set at 98 d, but in chicken muscle, its residues could only be detected for 4 d after ceasing treatment (50 mg/kg BW for 7 d).…”

Section: Discussionmentioning

confidence: 99%

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Gbylik-Sikorska¹,

Łebkowska‐Wieruszewska²,

Gajda³

et al. 2021

Poultry Science

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Regardless of whether antimicrobial drugs are administered to laying hens legally or illegally, residues of these drugs may be present in the eggs. Even if the eggs are not intended for human consumption, byproducts/biowaste, such as eggshells, may contain residues of the drugs used, which may pose a risk to human health and the environment. In the presented research, 2 different groups of laying hens received enrofloxacin (10 mg/kg body weight) and lincomycin (20 mg/kg body weight) once daily for 5 d. Eggs were collected daily and the concentration of enrofloxacin, its metabolite ciprofloxacin, and lincomycin residue in the eggshells, whole eggs, egg yolks, and egg whites were determined by ultra-high-performance liquid chromatography-tandem mass spectrometry. This study demonstrates the transfer of enrofloxacin, ciprofloxacin, and lincomycin into the eggshells and provides evidence for the distribution into the eggshells after administration of these drugs to laying hens. The enrofloxacin residues were detected in the eggshell for 10 d after cessation of treatment, ciprofloxacin and lincomycin were rapidly eliminated and 2 d after finish drugs administration they were no longer detected in the eggshell.

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Section: Discussionmentioning

confidence: 99%

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Gbylik-Sikorska¹,

Łebkowska‐Wieruszewska²,

Gajda³

et al. 2021

Poultry Science

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“…The internal standards (I.S.) ofloxacin-d 3 (OFL-d 3 , ≥ 99.0%), demeclocycline (DMC, ≥ 90.0%), sulfamethoxazole-(phenyl-13 C 6 ) (SMX- 13 C, ≥ 99.0%) and erythromycin-(N,N-dimethyl- 13 C 2 ) (ERY- 13 C, ≥ 99.0%) were purchased from Sigma-Aldrich (Steinheim, Germany). The structures of the target compounds, and their pK a and log K ow values, are presented in Table S1 in Electronic Supplementary Material (ESM).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…Pre-treated faeces samples (0.5 g dry weight (dw)) were spiked with the I.S. (OFL-d 3 , DMC, SMX- 13 C and ERY-13 C) at 100 ng g −1 dw. Samples were extracted three times with 5 mL of MeOH by sonication in an ultrasonic bath (25 °C, 80 kHz) for 10 min.…”

Section: Sample Collection and Treatmentmentioning

confidence: 99%

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Development and validation of a highly effective analytical method for the evaluation of the exposure of migratory birds to antibiotics and their metabolites by faeces analysis

et al. 2022

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The widespread occurrence of antibiotics in the environment may exert a negative impact on wild organisms. In addition, they can become environmental reservoirs, through the ingestion of food or contaminated water, and vectors for antibiotic-resistant bacteria. This fact is even more important in migratory birds that can promote their dissemination across continents. In this work, a multiresidue analytical method suitable for the determination of five families of antibiotics and their main metabolites in waterbird faeces has been developed and validated. The target compounds include environmentally significant sulfonamides, macrolides, fluoroquinolones, tetracyclines and antifolates. Sample treatment involves ultrasound-assisted extraction with methanol and dispersive solid-phase extraction clean-up with C18. Analytical determination was carried out by liquid chromatography–tandem mass spectrometry. The most significant parameters affecting sample extraction and extract clean-up were optimised by means of experimental designs. Good linearity (R2 > 0.994), accuracy (from 41 to 127%), precision (relative standard deviation lower than 24%) and limits of quantification (lower than 2 ng g−1 (dry weight, dw)) were obtained for most of the compounds. The method was applied to the determination of the selected compounds in 27 faeces samples from three common migratory waterbird species. Nine antibiotics and three of their metabolites were detected in the analysed samples. Fluoroquinolones and macrolides were the antibiotics most frequently detected. The highest concentrations corresponded to norfloxacin (up to 199 ng g−1 dw). Graphical abstract

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“…Spectinomycin has strong antibacterial activity against Gram-negative bacteria and weak activity against Gram-positive bacteria, whereas lincomycin has no effect on Gram-negative bacteria but a strong antibacterial effect on Gram-positive bacteria. Therefore, spectinomycin and lincomycin are usually used in combination to treat infections with Gram-positive bacteria and Gram-negative bacteria and are widely used to treat piglet diarrhea and infection by Mycoplasma hyopneumoniae and Mycoplasma pneumoniae, which cause chronic respiratory diseases in chickens [3][4][5][6]. However, spectinomycin can damage the eighth cranial nerve, exert kidney toxicity and block neuromuscular transmission; lincomycin has strong side effects, damages the gastrointestinal tract and liver, and even causes anaphylactic shock and death.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Spectinomycin and Lincomycin in Poultry Eggs by Accelerated Solvent Extraction Coupled with Gas Chromatography Tandem Mass Spectrometry

Wang

Xie

et al. 2020

Foods

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A method based on accelerated solvent extraction (ASE) coupled with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed for the quantitative analysis of spectinomycin and lincomycin in poultry egg (whole egg, albumen and yolk) samples. In this work, the samples were extracted and purified using an ASE350 instrument and solid-phase extraction (SPE) cartridges, and the parameters of the ASE method were experimentally optimized. The appropriate SPE cartridges were selected, and the conditions for the derivatization reaction were optimized. After derivatization, the poultry egg (whole egg, albumen and yolk) samples were analyzed by GC-MS/MS. This study used blank poultry egg (whole egg, albumen and yolk) samples to evaluate the specificity, sensitivity, linearity, recovery and precision of the method. The linearity (5.6–2000 μg/kg for spectinomycin and 5.9–200 μg/kg for lincomycin), correlation coefficient (≥0.9991), recovery (80.0%–95.7%), precision (relative standard deviations, 1.0%–3.4%), limit of detection (2.3–4.3 μg/kg) and limit of quantification (5.6–9.5 μg/kg) of the method met the requirements for EU parameter verification. Compared with traditional liquid–liquid extraction methods, the proposed method is fast and consumes less reagents, and 24 samples can be processed at a time. Finally, the feasibility of the method was evaluated by testing real samples, and spectinomycin and lincomycin residues in poultry eggs were successfully detected.

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Assessing the depletion of lincomycin in feathers from treated broiler chickens: a comparison with the concentration of its residues in edible tissues

Cited by 7 publications

References 24 publications

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens

Development and validation of a highly effective analytical method for the evaluation of the exposure of migratory birds to antibiotics and their metabolites by faeces analysis

Quantitative Analysis of Spectinomycin and Lincomycin in Poultry Eggs by Accelerated Solvent Extraction Coupled with Gas Chromatography Tandem Mass Spectrometry

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